LOCUS       sequence-436840-        7058 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector BS2 in nuclear pore.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7058)
  AUTHORS   Pani S, Qiu T, Kentala K, Azizi SA, Dickinson BC
  TITLE     Bioorthogonal masked acylating agents for proximity-dependent RNA 
            labelling.
  JOURNAL   Nat Chem. 2024 Apr 9. doi: 10.1038/s41557-024-01493-1.
  PUBMED    38594368
REFERENCE   2  (bases 1 to 7058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem. 
            2024 Apr 9. doi: 10.1038/s41557-024-01493-1."
COMMENT     Sequence Label: V5-BS2-SENP2 (Nuclear Pore)
FEATURES             Location/Qualifiers
     source          1..7058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1108..1149)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     regulatory      1149..1158
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1200..1219)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          1202..1334
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        complement(1470..1673)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1499..1519)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1674..2053
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    2244..2527
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     rep_origin      2643..3231
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3132..3151
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3403..4197)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MLEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFAKLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3514..3533)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4124..4143
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     polyA_signal    4596..4717
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4633..4652)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4687..4706
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4754..4772
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ataggcgacg gcagggcgcc aaaataataa atcagtcatc atgtgaattt ggctttccag
       61 agaacgcgga tacaaatcgg caactttctc tgccagcggc ttccctagta aatactcgag
      121 cgctgcatca agcgtttcct gagaatgaac gtctgaatcc ggggtgaaaa ataaatatcc
      181 ttcatcacgg gttgttccaa ttaatagcgg aataccggaa gcagcccctt ctgcgatcgc
      241 tttttctggt tcttcaggca gcgttttcgg atcaagggcg ggctggaaga acagctgaaa
      301 gatattttct ttttctgcaa tccgaagctg atcagccgct tttagcaaat cttccgcaga
      361 aaccgtatgc aatttatcca gttggccctc gttaatccca aggacctgta aaaaggctgc
      421 cgaggtgctc gccgcttgtt ctttcgtcat cgttcgagaa gcgccgcttt ccatgattgc
      481 tttctggaac aggccttttg ccgcaggcat agcaagcagc gcggcaatgc tcatcccgcc
      541 ggcggattct ccaaatactg ttacgttatc gggatcaccg ccaaacgctg aaatattctc
      601 tcgcacccat ttcagcgcgg cggcttggtc taaaagccca aggttatcag aatacgcctc
      661 attaaatgaa gacaagtgca aaaagccaaa cggccccagc cgatagttca atgtaacgac
      721 aatgacttct ccctgtgccg caagttttga tccgtcatac aatggctcac tgcccgctcc
      781 tagataaaaa gcgcctccgt gaatccacac catgacagga agatttttac ttggggtgtc
      841 aggcgcaaat acattgacat acaagcaatc ctcggactgg cggggcagct cagtatacga
      901 aagtgacagc aaatcagacg gctgcgggca aatagagccg tacgctgtgg catcaagcac
      961 atcttcccac acttcaggcg gctcaggtgc tttaaaacgc cattgtccga caggcggctt
     1021 ggcatagggg atgcctttcc acttatgtac gccgttttcc gttgtgcctt ttactttgcc
     1081 gtattgagtc gttactattt gatgagtggt gctgtccagg cccagcaggg ggttggggat
     1141 gggcttgccc atggtggcat actctagcct taagagctgt aattgaactg ggagtggaca
     1201 cctgtggaga gaaaggcaaa gtggatgtca gtaagaccaa taggtgccta tcagaaacgc
     1261 aagagtcttc tctgtctcga caagcccagt ttctattggt ctccttaaac ctgtcttgta
     1321 accttgatac ttacctgccc agtgcctcac gaccaacttc tgcagcttaa gttcgagact
     1381 gttgtgtcag aagcactgac tgcgttagca atttaactgt gataaactac cgcaataaag
     1441 cttctagtga tctgacggtt cactaaacca gctctgctta tatagacctc ccaccgtaca
     1501 cgcctaccgc ccatttgcgt caacggggcg gggttattac gacattttgg aaagtcccgt
     1561 tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc
     1621 cgtgagtcaa accgctatcc acgcccattg gtgtactgcc aaaaccgcat caccatggta
     1681 atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccgtaa ggtcatgtac
     1741 tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg cggacttggc
     1801 atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga
     1861 cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat
     1921 gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg
     1981 aactccatat atgggctatg aactaatgac cccgtaattg attactatta ataactagtc
     2041 aataatcaat gccaacatgg cggtcatatt ggacatgagc caatataaat gtacatatta
     2101 tgatatagat acaacgtatg caatggccaa tagccaatat tgatttatgc tatataacca
     2161 atgaataata tggctaatgg ccaatattga agatctcgat cctctacgcc ggacgcatcg
     2221 tggccggagt actgcggttt atctcgatgg ctacgagggc agacagtaag tggatttacc
     2281 ataatccctt aattgtacgc accgctaaaa cgcgttcagc gcgatcacgg cagcagacag
     2341 gtaaaaatgg caacaaacca cccgaaaaac tgccgcaatc gcgcctgata aattttaacc
     2401 gtatgaatac ctatgcaacc agagggtaca ggccacatta cccccactta atccactgaa
     2461 gctgccattt ttcatggttt caccatccca gcgaagggcc atccagcgtg cgttcctgta
     2521 tttccggact ataaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     2581 acttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2641 tcttgaaatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2701 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2761 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     2821 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2881 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2941 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3001 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3061 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3181 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3241 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3301 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccggt
     3361 tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag
     3421 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa
     3481 gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc
     3541 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt
     3601 ttccaccatg atattcggca agcaggcatc gccatgagtc acgacgagat cctcaccatc
     3661 cggcatacgc gccttgagct tggcgaacag ttcggctggc gcgagcccct gatgctcttc
     3721 gtccagatca tcctgatcga caagaccggc ttccatccga gtgcgtgctc gctcgatgcg
     3781 atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat
     3841 tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg
     3901 ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac
     3961 agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag
     4021 ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga
     4081 cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa
     4141 tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caagcatgct
     4201 aaacgaccgt catcctgtct cttgatcaga tcttgatccc ctgcgccatc agatccttgg
     4261 cggcaagaaa gccatccagt ttactttgca gggcttccca accttaccag agggcgcccc
     4321 agctggcaat tcttttgaag ctcacgctgc cgcaagcact cagggcgcaa gggctgctaa
     4381 aggaagcgga acacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc
     4441 agctactggg ctatctggac aagggaaaac gcaagcgcaa agaggatcca ttagaattcg
     4501 attttaccac atttgtagag gttttacttg cttaaaaaaa cctcccacat ctccccctga
     4561 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     4621 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     4681 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgctcg aagcggccgc
     4741 cccaaggggt tatgctagtt attgctcagc ggtggcagca gccaactcag cttcctttcg
     4801 ggctttgtta gcagccggat cagcttgcat gcctgcaggt cgactctaga ggatccccgg
     4861 gtaccgagcc cgaattcgtt taaacattac agcaactgct gatgaaggat ttcccacacc
     4921 atcttcttcc ggaagagagg catctggtgc tgagtaaatg tgataggttt gtccctagaa
     4981 atataatctg catatttaca agtaaacatt ccacaatcac tcccattcag ctgttgagga
     5041 atctcgtgtg gtttcatgct gtgatgggtc cactctaaaa gattcagatc actatttctt
     5101 ttggtcttac tttcatcctg taaatactga aggagaatct cacagatcct gtggcccttt
     5161 tgtcccatag aatccagata tttaagacac ttttttctta ggtcaatcac caccaggctc
     5221 caatgtacct tccgatgaat aggcaccaga ataatttctt gttcaaagag atttacccct
     5281 ttggtccatc gtttcactgc ttggtaaccc ccagacttta atttaggata gaagaaagta
     5341 ctgaatacat gaagtgctgg atagccttgc tttttatttc tttccaccag aagattcatg
     5401 taaaaattaa tgacttcatc attgagccag tgatagttct ttaatgtctg aatatctcct
     5461 cgagtaattc gcaatttgaa agcactactt aggatttcat cctgtgggcc atggcctagg
     5521 gcattactga tttccttttc catgtcctct gtaagttcaa ggagatcgtc cgttcttctg
     5581 tccctctctt tgcctgagca attcttttcc tttgtctcaa ttattgacac tttcctcctg
     5641 agtaagccat tgcttccact gcccaggcgg agtcgggccg acacttcttc tgataggtca
     5701 ggctccagtt ggtatcccct cctactttca ttttcaaatc tgattccgac catcgtctct
     5761 gtatcagtaa ttttcccctt tgaacacctc ttttcacttc tcaatgaaca tagaggtccc
     5821 cttgtttcaa caagtctata ttgttttgga acaaactgag ttgttttgac tccgtgattt
     5881 tgctcttccc cccagccttt ggtctttaat gtgtccatct gacttctttg agaactgtga
     5941 taatttgaag ttacaggaca gacagagttt ccatgaccac tttctttaag tcgttccaat
     6001 aactttcggt acttctctct ttcctctttt tgaacaccct cctccacagt acaatgggga
     6061 cgcctcagac ccttgccact ctcttcagaa atcatctctg ttacagcctg ttcctgaggt
     6121 ttccacatta aagaactctc tggattacct ttgctatggc gacggccacc tggtcttcta
     6181 ttacagcctt ctgagttcaa agtaaaacca aaggaaggca ggactctgcg tggctgatct
     6241 cgggtaactg tcactctgat ctttggatag tcacttattc cattaggaga tttattaccc
     6301 agtttcagca tgttgttcca ggatccagaa cctgtcagtt cacaagatga agagttcgaa
     6361 aatacctctc ctgaaggggc cacattccgt gttccattac aagcagaagt taccatgggc
     6421 tttgtggtca gctggaatgg gaatccaaat aagctggcag cattgtagag actgtttttc
     6481 acttggtgaa taaagcaatc taatcttggt cttttggctg gtatttcatc agtgtccact
     6541 gtagaaaaca gagtgctgtc tgagcgccgc ctcttcagga gggcccgggc agggggcacc
     6601 gaccggtcgc agaaacggaa aatggtgccg agaatcctaa ccagccatct gtacatgaat
     6661 tcgaagcttg agctcgagat ctgagtccgg ccggaggatc cttctccttt tgaagggaat
     6721 agcttctgcc ttttttcaga ttcgggatcg ttttcgatcg taatctctga gtctaaaatc
     6781 agcgtctctc tcgtttcttc atgatacgca ggccaattca cagcttcggt gcttgggttt
     6841 cctgttttgg cgaacgtgat ccacgctgat tgtatcgtgt gagaaagctg tttcacctca
     6901 tccgtaatct ccgcttttgc cattcgttcc aatccgtcca gatttccaaa gacaaaagga
     6961 agctctaatg cgtgaaacgc tttattgtac ggcggcttct tcgggtgcca atcgaacctg
     7021 tacatccaga caggggcgta atgagactgt gcggatgc
//
