LOCUS       sequence-43685-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbB1k-GFP
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      49..120
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      136..163
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 tgagctctac aaataaggat ccaaactcga gtaaggatct ccaggcatca aataaaacga
       61 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
      121 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatac ctaggctaca
      181 gccgatagtc tggaacagcg cacttacggg ttgctgcgca acccaagtgc taccggcgcg
      241 gcagcgtgac ccgtgtcggc ggctccaacg gctcgccatc gtccagaaaa cacggctcat
      301 cgggcatcgg caggcgctgc tgcccgcgcc gttcccattc ctccgtttcg gtcaaggctg
      361 gcaggtctgg ttccatgccc ggaatgccgg gctggctggg cggctcctcg ccggggccgg
      421 tcggtagttg ctgctcgccc ggatacaggg tcgggatgcg gcgcaggtcg ccatgcccca
      481 acagcgattc gtcctggtcg tcgtgatcaa ccaccacggc ggcactgaac accgacaggc
      541 gcaactggtc gcggggctgg ccccacgcca cgcggtcatt gaccacgtag gccaacacgg
      601 tgccggggcc gttgagcttc acgacggaga tccagcgctc ggccaccaag tccttgactg
      661 cgtattggac cgtccgcaaa gaacgtccga tgagcttgga aagtgtcttc tggctgacca
      721 ccacggcgtt ctggtggccc atctgcgcca cgaggtgatg cagcagcatt gccgccgtgg
      781 gtttcctcgc aataagcccg gcccacgcct catgcgcttt gcgttccgtt tgcacccagt
      841 gaccgg
//
