LOCUS       sequence-43686-e         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbB1k-GFP
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    40..56
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(64..70,71..87,88..93))
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 tgtatatctc cttcttaaaa gatcttttga attctgaaat tgttatccgc tcacaattcc
       61 acacattata cgagccggat gattaattgt caacagctca tttcagaata tttgccagaa
      121 ccgttatgat gtcggcgcaa aaaacattat ccagaacggg agtgcgcctt gagcgacacg
      181 aattatgcag tgatttacga cctgcacagc cataccacag cttccgatgg ctgcctgacg
      241 ccagaagcat tggtgcaccg tgcagtcgat gataagctgt caaaccagat caattcgcgc
      301 taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
      361 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag
      421 ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc
      481 ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt
      541 gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac
      601 cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc
      661 catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat
      721 ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat
      781 ttgattgcga gtgagatatt tatg
//
