LOCUS       sequence-43688-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbB2a-GFP
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        36..91
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    37..55
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             107..733
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(183..202)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        765..869
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtatatctcc ttcttaaaag atcttttgaa ttcttttctc tatcactgat agggagtggt
       61 aaaataactc tatcaacgat agagtgtcaa caaaaattag gaattaatga tgtctagatt
      121 agataaaagt aaagtgatta acagcgcatt agagctgctt aatgaggtcg gaatcgaagg
      181 tttaacaacc cgtaaactcg cccagaagct aggtgtagag cagcctacat tgtattggca
      241 tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt gagatgttag ataggcacca
      301 tactcacttt tgccctttag aaggggaaag ctggcaagat tttttacgta ataacgctaa
      361 aagttttaga tgtgctttac taagtcatcg cgatggagca aaagtacatt taggtacacg
      421 gcctacagaa aaacagtatg aaactctcga aaatcaatta gcctttttat gccaacaagg
      481 tttttcacta gagaatgcat tatatgcact cagcgctgtg gggcatttta ctttaggttg
      541 cgtattggaa gatcaagagc atcaagtcgc taaagaagaa agggaaacac ctactactga
      601 tagtatgccg ccattattac gacaagctat cgaattattt gatcaccaag gtgcagagcc
      661 agccttctta ttcggccttg aattgatcat atgcggatta gaaaaacaac ttaaatgtga
      721 aagtgggtct taagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      781 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      841 tgcttcaata atattgaaaa aggaagagta t
//
