LOCUS       sequence-436883-       13721 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible V5-tagged luciferase 
            construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13721)
  TITLE     Janes lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSLIK luciferase-V5 neo
FEATURES             Location/Qualifiers
     source          1..13721
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(21..45)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    102..219
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(517..561)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    746..979
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1472..1597
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1644..1824
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1842..2040)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        2042..2421
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     2593..2612
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        2687..2791
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2792..2860,2861..3652)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3010..3029)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3823..4411
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4312..4331
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4565..4582
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4699..4720
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4735..4740,4741..4758,4759..4765)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4773..4789
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4778..4800
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4797..4813
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4797..4813
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    4862..4983
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4873..4892)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4927..4946
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5113..5487)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(5506..5553)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(5601..5930)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5615..5750
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5669..5688)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     5915..5935
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(5944..6372)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6054..6075)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6266..6285
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    6418..6642
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     6631..6648
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             6674..6854
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     7363..7379
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7364..7380
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    7383..7971
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7489..7500
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7898..7918
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8139..8933)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(8139..8933)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8250..8269)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8860..8879
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_feature    8949..9522
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9109..9128)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     9338..9355
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(join(9575..9583,9584..9619,9620..9622,9623..9658,
                     9659..9661,9662..9697,9698..9703,9704..10321))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(10380..11591)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(10524..10543)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    11641..11665
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(11704..11745)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     13305..13323
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     promoter        complement(13396..13710)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(13399..13423)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    13470..13488
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    13506..13524
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    13541..13559
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    13577..13595
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    13613..13631
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    13648..13666
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    13684..13702
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 gactgaattg gttcctttaa agcctgcttt tttgtacaaa cttgttgata actgtctaga
       61 attaaccaaa ctggatctct gctgtccctg taataaaccc gaaaattttg aatttttgta
      121 atttgttttt gtaattcttt agtttgtatg tctgttgcta ttatgtctac tattctttcc
      181 cctgcactgt accccccaat cccccctttt cttttaaaat tgtggatgaa tactgccatt
      241 tgtctgcaga attggcgcac gcagtgccga tccgttcact aatcgaatgg atctgtctct
      301 gtctctctct ccaccttctt cttctattcc ttcgggcctg tcgggtcccc tcggggttgg
      361 gaggtgggtc tgaaacgata atggtgaata tccctgccta actctattca ctatagaaag
      421 tacagcaaaa actattctta aacctaccaa gcctcctact atcattatga ataattttat
      481 ataccacagc caatttgtta tgttaaacca attccacaaa cttgcccatt tatctaattc
      541 caataattct tgttcattct tttcttgctg gttttgcgat tcttcaatta aggagtgtat
      601 taagcttgtg taattgttaa tttctctgtc ccactccatc caggtcgtgt gattccaaat
      661 ctgttccaga gatttattac tccaactagc attccaaggc acagcagtgg tgcaaatgag
      721 ttttccagag caaccccaaa tccccaggag ctgttgatcc tttaggtatc tttccacagc
      781 caggattctt gcctggagct gcttgatgcc ccagactgtg agttgcaaca gatgctgttg
      841 cgcctcaata gccctcagca aattgttctg ctgctgcact ataccagaca ataattgtct
      901 ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg cttcctgctg ctcccaagaa
      961 cccaaggaac aaagctccta ttcccactgc tcttttttct ctctgcacca ctcttctctt
     1021 tgccttggtg ggtgctactc ctaatggttc aatttttact actttatatt tatataattc
     1081 acttctccaa ttgtccctca tatctcctcc tccaggtctg aagatcagcg gccgcttgct
     1141 gtgcggtggt cttacttttg ttttgctctt cctctatctt gtctaaagct tccttggtgt
     1201 cttttatctc tatcctttga tgcacacaat agagggttgc tactgtatta tataatgatc
     1261 taagttcttc tgatcctgtc tgaagggatg gttgtagctg tcccagtatt tgtctacagc
     1321 cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg ttctagctcc ctgcttgccc
     1381 atactatatg ttttaattta tattttttct ttccccctgg ccttaaccga attttttccc
     1441 atcgcgatct aattctcccc cgcttaatac tgacgctctc gcacccatct ctctccttct
     1501 agcctccgct agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg
     1561 tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc ctctggtttc cctttcgctt
     1621 tcaagtccct gttcgggcgc cactgctaga gattttccac actgactaaa agggtctgag
     1681 ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg
     1741 caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca
     1801 gatctggtct aaccagagag acccagtaca ggcaaaacgc gctgcttata tagacctccc
     1861 accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa
     1921 agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg
     1981 gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca
     2041 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
     2101 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
     2161 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
     2221 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
     2281 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
     2341 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
     2401 aactagtcaa taatcaatgt caacgcgtat atctggcccg tacatcgcga agcagcgcaa
     2461 aacgcctaac cctaagcaga ttcttcatgc aattgtcggt caagccttgc cttgttgtag
     2521 cttaaatttt gctcgcgcac tactcagcga cctccaacac acaagcaggg agcagatact
     2581 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatagg ggatcgggag
     2641 atctcccgat ccgtcgacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     2701 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     2761 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     2821 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     2881 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     2941 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     3001 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     3061 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     3121 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     3181 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     3241 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     3301 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     3361 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     3421 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     3481 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     3541 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     3601 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     3661 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     3721 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     3781 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3841 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3901 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3961 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     4021 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     4081 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4141 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     4201 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     4261 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4321 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4381 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     4441 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     4501 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     4561 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     4621 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     4681 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     4741 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     4801 aaacagctat gaccatgatt acgccaagct ctagctagag gtcgacggta tacagacatg
     4861 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     4921 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     4981 gttggggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc
     5041 gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc
     5101 tcgtagcacg tgtcagtcct gctcctcggc cacgaagtgc acgcagttgc cggccgggtc
     5161 gcgcagggcg aactcccgcc cccacggctg ctcgccgatc tcggtcatgg ccggcccgga
     5221 ggcgtcccgg aagttcgtgg acacgacctc cgaccactcg gcgtacagct cgtccaggcc
     5281 gcgcacccac acccaggcca gggtgttgtc cggcaccacc tggtcctgga ccgcgctgat
     5341 gaacagggtc acgtcgtccc ggaccacacc ggcgaagtcg tcctccacga agtcccggga
     5401 gaacccgagc cggtcggtcc agaactcgac cgctccggcg acgtcgcgcg cggtgagcac
     5461 cggaacggca ctggtcaact tggccatggt ttagttcctc accttgtcgt attatactat
     5521 gccgatatac tatgccgatg attaattgtc aacacgtgct gatcagatcc gaaaatggat
     5581 atacaagctc ccgggagctt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac
     5641 ttctggaata gctcagaggc agaggcggcc tcggcctctg cataaataaa aaaaattagt
     5701 cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt
     5761 aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
     5821 ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata
     5881 cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac attccacaga
     5941 attaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     6001 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     6061 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     6121 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     6181 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     6241 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     6301 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     6361 tacagggcgc gtggggatac cccctagagc cccagctggt tctttccgcc tcagaagcca
     6421 tagagcccac cgcatcccca gcatgcctgc tattgtcttc ccaatcctcc cccttgctgt
     6481 cctgccccac cccacccccc agaatagaat gacacctact cagacaatgc gatgcaattt
     6541 cctcatttta ttaggaaagg acagtgggag tggcaccttc cagggtcaag gaaggcacgg
     6601 gggaggggca aacaacagat ggctggcaac tagaaggcac agtcgaggct gatcagcggg
     6661 tttaaacggg ccctgctaga gattttccac actgactaaa agggtctgag ggatctctag
     6721 ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat
     6781 tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct
     6841 aaccagagag acccagtaca gtccggatgc agctctcggg ccatgtgatg aaatgctagg
     6901 cggctgtcaa acctccactc taatacttct ctctccgggt catccatccc atgcaggctc
     6961 acagggtgta acaagcgggt gttctctcct tcattggctt cttctacctt ctcttgctca
     7021 actggtacta gcttgtagca ccatccaaag gtcagtggat atctgatccc tggccctggt
     7081 gtgtagttct gccaatcagg gaagtagcct tgtgtgtggt agatccacag atcaaggata
     7141 tcttgtcttc gttgggagtg aattagccct tccagtcccc ccttttcttt taaaaagtgg
     7201 ctaagatcta cagctgcctt gtaagtcatt ggtcttaaag gtacctgagg tgtgactgga
     7261 aaacccacct cctcctcctc ttgtgcttct agccaggcac aatcagcatt ggtagctgct
     7321 gtattgctac ttgtgattgc tccatgtttt tctaggtctc gatcgaggtc gacggtatcg
     7381 atgcggggag gcggcccaaa gggagatccg actcgtctga gggcgaaggc gaagacgcgg
     7441 aagaggccgc agagccggca gcaggccgcg ggaaggaagg tccgctggat tgagggccga
     7501 agggacgtag cagaaggacg tcccgcgcag aatccaggtg gcaacacagg cgagcagcca
     7561 aggaaaggac gatgatttcc ccgacaacac cacggaattg tcagtgccca acagccgagc
     7621 ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc gccgtggcaa tagggagggg
     7681 gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca atgccccaac cagtgggggt
     7741 tgcgtcagca aacacagtgc acaccacgcc acgttgcctg acaacgggcc acaactcctc
     7801 ataaagagac agcaaccagg atttatacaa ggaggagaaa atgaaagcca tacgggaagc
     7861 aatagcatga tacaaaggca ttaaagcagc gtatccacat agcgtaaaag gagcaacata
     7921 gttaagaata ccagtcaatc tttcacaaat tttgtaatcc agaggttgat tatcgataag
     7981 cttgatatcg aattgggagg gactaaacaa caacaattgc attcatttta tgtttcaggt
     8041 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
     8101 tgattatgat ctagagtcgc ggccgcttta cttgtacatc agaagaactc gtcaagaagg
     8161 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg
     8221 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga
     8281 tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc
     8341 accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc
     8401 atgctcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc
     8461 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt
     8521 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca
     8581 tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc
     8641 ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct
     8701 gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttgcagttca
     8761 ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc
     8821 cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc
     8881 ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat catggtggac
     8941 cggtaagctt atcatcgtgt ttttcaaagg aaaaccacgt ccccgtggtt cggggggcct
     9001 agacgttttt ttaacctcga ctaaacacat gtaaagcatg tgcaccgagg ccccagatca
     9061 gatcccatac aatggggtac cttctgggca tccttcagcc ccttgttgaa tacgcttgag
     9121 gagagccatt tgactctttc cacaactatc caactcacaa cgtggcactg gggttgtgcc
     9181 gcctttgcag gtgtatctta tacacgtggc ttttggccgc agaggcacct gtcgccaggt
     9241 ggggggttcc gctgcctgca aagggtcgct acagacgttg tttgtcttca agaagcttcc
     9301 agaggaactg cttccttcac gacattcaac agaccttgca ttcctttggc gagaggggaa
     9361 agacccctag gaatgctcgt caagaagaca gggccaggtt tccgggccct cacattgcca
     9421 aaagacggca atatggtgga aaataacata tagacaaacg cacaccggcc ttattccaag
     9481 cggcttcggc cagtaacgtt aggggggggg gagggagagg gggggggggg agaggggcgg
     9541 aattcctcta gtgcggccgc ggatccttac ttagttaccc ggggagcatg tcaaggtcaa
     9601 aatcgtcaag agcgtcagca ggcagcatat caaggtcaaa gtcgtcaagg gcatcggctg
     9661 ggagcatgtc taagtcaaaa tcgtcaaggg cgtcggtcgg cccgccgctt tcgcacttta
     9721 gctgtttctc caggccacat atgattagtt ccaggccgaa aaggaaggca ggttcggctc
     9781 cctgccggtc gaacagctca attgcttgtc tcagaagtgg gggcatagaa tcggtggtag
     9841 gtgtctctct ttcctctttt gctacttgat gctcctgttc ctccaatacg cagcccagtg
     9901 taaagtggcc cacggcggac agagcgtaca gtgcgttctc cagggagaag ccttgctgac
     9961 acaggaacgc gagctgattt tccagggttt cgtactgttt ctctgttggg cgggtgccga
    10021 gatgcacttt agccccgtcg cgatgtgaga ggagagcaca gcggtatgac ttggcgttgt
    10081 tccgcagaaa gtcttgccat gactcgcctt ccagggggca gaagtgggta tgatgcctgt
    10141 ccagcatctc gattggcagg gcatcgagca gggcccgctt gttcttcacg tgccagtaca
    10201 gggtaggctg ctcaactccc agcttttgag cgagtttcct tgtcgtcagg ccttcgatac
    10261 cgacaccatt gagtaattcc agagctccgt ttatgacttt gctcttgtcc agtctagaca
    10321 tggtgaattc gactgcagga ccggtacggg gtggagatcc gagctcggta ccaagcttcg
    10381 tctaacaaaa aagccaaaaa cggccagaat ttagcggaca atttactagt ctaacactga
    10441 aaattacata ttgacccaaa tgattacatt tcaaaaggtg cctaaaaaac ttcacaaaac
    10501 acactcgcca accccgagcg catagttcaa aaccggagct tcagctactt aagaagatag
    10561 gtacataaaa ccgaccaaag aaactgacgc ctcacttatc cctcccctca ccagaggtcc
    10621 ggcgcctgtc gattcaggag agcctaccct aggcccgaac cctgcgtcct gcgacggaga
    10681 aaagcctacc gcacacctac cggcaggtgg ccccaccctg cattataagc caacagaacg
    10741 ggtgacgtca cgacacgacg agggcgcgcg ctcccaaagg tacgggtgca ctgcccaacg
    10801 gcaccgccat aactgccgcc cccgcaacag acgacaaacc gagttctcca gtcagtgaca
    10861 aacttcacgt cagggtcccc agatggtgcc ccagcccatc tcacccgaat aagagctttc
    10921 ccgcattagc gaaggcctca agaccttggg ttcttgccgc ccaccatgcc ccccaccttg
    10981 tttcaacgac ctcacagccc gcctcacaag cgtcttccat tcaagactcg ggaacagccg
    11041 ccattttgct gcgctccccc caacccccag ttcagggcaa ccttgctcgc ggacccagac
    11101 tacagccctt ggcggtctct ccacacgctt ccgtcccacc gagcggcccg gcggccacga
    11161 aagccccggc cagcccagca gcccgctact caccaagtga cgatcacagc gatccacaaa
    11221 caagaaccgc gacccaaatc ccggctgcga cggaactagc tgtgccacac ccggcgcgtc
    11281 cttatataat catcggcgtt caccgcccca cggagatccc tccgcagaat cgccgagaag
    11341 ggactacttt tcctcgcctg ttccgctctc tggaaagaaa accagtgccc tagagtcacc
    11401 caagtcccgt cctaaaatgt ccttctgctg atactggggt tctaaggccg agtcttatga
    11461 gcagcgggcc gctgtcctga gcgtccgggc ggaaggatca ggacgctcgc tgcgcccttc
    11521 gtctgacgtg gcagcgctcg ccgtgaggag gggggcgccc gcgggaggcg ccaaaacccg
    11581 gcgcggaggc cagatcttgg gtgggttaat taaccatctt tatggctcga gatgttatca
    11641 accactttgt acaagaaagc tgggtctaga tatctcgaaa acgcattagt cttccaattc
    11701 tcacgtagaa tcgagaccga ggagagggtt agggataggc ttaccgatct ttccgccctt
    11761 cttggccttt atgaggatct ctctgatttt tcttgcgtcg agttttccgg taagaccttt
    11821 cggtacttcg tccacaaaca caactcctcc gcgcaacttt ttcgcggttg ttacttgact
    11881 ggcgacgtaa tccacgatct ctttttccgt catcgtcttt ccgtgctcca aaacaacaac
    11941 ggcggcggga agttcaccgg cgtcatcgtc gggaagacct gcgacacctg cgtcgaagat
    12001 gttggggtgt tggagcaaga tggattccaa ttcagcggga gccacctgat agcctttgta
    12061 cttaatcaga gacttcaggc ggtcaacgat gaagaagtgt tcgtcttcgt cccagtaagc
    12121 tatgtctcca gaatgtagcc atccatcctt gtcaatcaag gcgttggtcg cttccggatt
    12181 gtttacataa ccggacataa tcataggacc tctcacacac agttcgcctc tttgattaac
    12241 gcccagcgtt ttcccggtat ccagatccac aaccttcgct tcaaaaaatg gaacaacttt
    12301 accgaccgcg cccggtttat catccccctc gggtgtaatc agaatagctg atgtagtctc
    12361 agtgagccca tatccttgcc tgatacctgg cagatggaac ctcttggcaa ccgcttcccc
    12421 gacttcctta gagaggggag cgccaccaga agcaatttcg tgtaaattag ataaatcgta
    12481 tttgtcaatc agagtgcttt tggcgaagaa ggagaatagg gttggcacca gcagcgcact
    12541 ttgaatcttg taatcctgaa ggctcctcag aaacagctct tcttcaaatc tatacattaa
    12601 gacgactcga aatccacata tcaaatatcc gagtgtagta aacattccaa aaccgtgatg
    12661 gaatggaaca acacttaaaa tcgcagtatc cggaatgatt tgattgccaa aaataggatc
    12721 tctggcatgc gagaatctca cgcaggcagt tctatgaggc agagcgacac ctttaggcag
    12781 accagtagat ccagaggagt tcatgatcag tgcaattgtc ttgtccctat cgaaggactc
    12841 tggcacaaaa tcgtattcat taaaaccggg aggtagatga gatgtgacga acgtgtacat
    12901 cgactgaaat ccctggtaat ccgttttaga atccatgata ataatttttt ggatgattgg
    12961 gagctttttt tgcacgttca aaattttttg caaccccttt ttggaaacga acaccacggt
    13021 aggctgcgaa atgcccatac tgttgagcaa ttcacgttca ttataaatgt cgttcgcggg
    13081 cgcaactgca actccgataa ataacgcgcc caacaccggc ataaagaatt gaagagagtt
    13141 ttcactgcat acgacgattc tgtgatttgt attcagccca tatcgtttca tagcttctgc
    13201 caaccgaacg gacatttcga agtactcagc gtaagtgatg tccacctcga tatgtgcatc
    13261 tgtaaaagca attgttccag gaaccagggc gtatctcttc atagccttat gcagttgctc
    13321 tccagcggtt ccatcttcca gcggatagaa tggcgccggg cctttcttta tgtttttggc
    13381 gtcttccata ctagtggcga tctgacggtt cactaaacga gctctgctta tataggcctc
    13441 ccaccgtaca cgcctacctc gacatacgtt ctctatcact gatagggagt aaactcgaca
    13501 tacgttctct atcactgata gggataaact cgacatacgt tctctatcac tgatagggag
    13561 taaactcgac atacgttctc tatcactgat agggagtaaa ctcgacatac gttctctatc
    13621 actgataggg agtaaactcg acatcgttct ctatcactga tagggagtaa actcgacata
    13681 cgttctctat cactgatagg gagtaaactc gggatccagt c
//
