LOCUS       sequence-436884-       13831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible V5-tagged CXADR 
            construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13831)
  TITLE     Janes lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSLIK CXADR-V5 hygro
FEATURES             Location/Qualifiers
     source          1..13831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    588..1176
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(641..661)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1059..1070)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1179..1195)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1180..1196)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             1705..1885
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(1911..1928)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1917..2141
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2187..2615
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2274..2293)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2484..2505
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2624..2644)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2629..2958
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2809..2944
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2871..2890
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3006..3053
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3072..3446
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    3576..3697
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3613..3632)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3667..3686
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3746..3762)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3746..3762)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3759..3781)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3770..3786
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3794..3800,3801..3818,3819..3824))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3839..3860
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3977..3994)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4148..4736)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4228..4247)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4907..5698,5699..5767))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5530..5549
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5768..5872)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5947..5966)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6138..6517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6519..6717
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             6735..6915
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6962..7087
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7580..7813
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7998..8042
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8340..8457
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    8514..8538
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        8570..8884
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    8578..8596
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8614..8632
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8649..8667
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8685..8703
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8721..8739
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8756..8774
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8792..8810
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     8857..8881
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(9987..10011)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             10013..10054
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(10093..10117)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        10167..11378
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     11215..11234
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(11503..12120,12121..12126,12127..12162,12163..12165,
                     12166..12201,12202..12204,12205..12240,12241..12249)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    12302..12875
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(12469..12486)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     12696..12715
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(12934..13831,1..122)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             join(12934..13831,1..122)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
ORIGIN
        1 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg
       61 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat
      121 agagtagatg ccgaccgaac aagagctgat ttcgagaacg cctcagccag caactcgcgc
      181 gagcctagca aggcaaatgc gagagaacgg ccttacgctt ggtggcacag ttctcgtcca
      241 cagttcgcta agctcgctcg gctgggtcgc gggagggccg gtcgcagtga ttcaggccct
      301 tctggattgt gttggtcccc agggcacgat tgtcatgccc acgcactcgg gtgatctgac
      361 tgatcccgca gattggagat cgccgcccgt gcctgccgat tgggtgcaga tctctagatc
      421 tgtacaagta aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt
      481 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
      541 aattgttgtt gtttagtccc tcccaattcg atatcaagct tatcgataat caacctctgg
      601 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
      661 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
      721 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
      781 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
      841 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
      901 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
      961 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     1021 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     1081 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     1141 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgatcga
     1201 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     1261 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     1321 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     1381 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
     1441 cacacacaag gctacttccc tgattggcag aactacacac cagggccagg gatcagatat
     1501 ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa
     1561 gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac
     1621 ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc
     1681 cgagagctgc atccggactg tactgggtct ctctggttag accagatctg agcctgggag
     1741 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1801 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1861 tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt
     1921 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     1981 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     2041 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     2101 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     2161 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     2221 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     2281 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     2341 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     2401 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     2461 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     2521 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     2581 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     2641 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     2701 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     2761 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     2821 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     2881 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     2941 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
     3001 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     3061 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
     3121 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
     3181 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
     3241 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
     3301 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
     3361 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
     3421 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
     3481 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     3541 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     3601 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     3661 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     3721 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     3781 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     3841 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     3901 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     3961 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     4021 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     4081 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     4141 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     4201 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     4261 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     4321 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     4381 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     4441 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     4501 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     4561 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     4621 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     4681 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     4741 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     4801 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     4861 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     4921 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     4981 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     5041 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     5101 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     5161 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     5221 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     5281 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     5341 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     5401 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     5461 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     5521 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     5581 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     5641 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     5701 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     5761 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     5821 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     5881 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac
     5941 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
     6001 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
     6061 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
     6121 ggccagatat acgcgttgac attgattatt gactagttat taatagtaat caattacggg
     6181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     6241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     6301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     6361 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     6421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     6481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     6541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     6601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     6661 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagcgc
     6721 gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6781 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6841 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6901 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     6961 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     7021 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     7081 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     7141 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     7201 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     7261 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     7321 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     7381 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     7441 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     7501 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     7561 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     7621 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     7681 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     7741 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     7801 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     7861 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     7921 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     7981 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     8041 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     8101 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     8161 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
     8221 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
     8281 tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt catccacaat
     8341 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     8401 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
     8461 gtttattaca gggacagcag agatccagtt tggttaattc tagacagtta tcaacaagtt
     8521 tgtacaaaaa agcaggcttt aaaggaacca attcagtcga ctggatcccg agtttactcc
     8581 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgatgtc
     8641 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
     8701 agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttatccct
     8761 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
     8821 aggtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat
     8881 cgccactagt atggcgctcc tgctgtgctt cgtgctcctg tgcggagtag tggatttcgc
     8941 cagaagtttg agtatcacta ctcctgaaga gatgattgaa aaagccaaag gggaaactgc
     9001 ctatctgccg tgcaaattta cgcttagtcc cgaagaccag ggaccgctgg acatcgagtg
     9061 gctgatatca ccagctgata atcagaaggt ggatcaagtg attattttat attctggaga
     9121 caaaatttat gatgactact atccagatct gaaaggccga gtacatttta cgagtaatga
     9181 tctcaaatct ggtgatgcat caataaatgt aacgaattta caactgtcag atattggcac
     9241 atatcagtgc aaagtgaaaa aagctcctgg tgttgcaaat aagaagattc atctggtagt
     9301 tcttgttaag ccttcaggtg cgagatgtta cgttgatgga tctgaagaaa ttggaagtga
     9361 ctttaagata aaatgtgaac caaaagaagg ttcacttcca ttacagtatg agtggcaaaa
     9421 attgtctgac tcacagaaaa tgcccacttc atggttagca gaaatgactt catctgttat
     9481 atctgtaaaa aatgcctctt ctgagtactc tgggacatac agctgtacag tcagaaacag
     9541 agtgggctct gatcagtgcc tgttgcgtct aaacgttgtc cctccttcaa ataaagctgg
     9601 actaattgca ggagccatta taggaacttt gcttgctcta gcgctcattg gtcttatcat
     9661 cttttgctgt cgtaaaaagc gcagagaaga aaaatatgaa aaggaagttc atcacgatat
     9721 cagggaagat gtgccacctc caaagagccg tacgtccact gccagaagct acatcggcag
     9781 taatcattca tccctggggt ccatgtctcc ttccaacatg gaaggatatt ccaagactca
     9841 gtataaccaa gtaccaagtg aagactttga acgcactcct cagagtccga ctctcccacc
     9901 tgctaaggta gctgccccta atctaagtcg aatgggtgcg attcctgtga tgattccagc
     9961 acagagcaag gatgggtcta tagtataccc aactttcttg tacaaagtgg ttggtaagcc
    10021 tatccctaac cctctcctcg gtctcgattc tacgtagcaa ttggaagact aatgcgtttt
    10081 cgagatatct agacccagct ttcttgtaca aagtggttga taacatctcg agccataaag
    10141 atggttaatt aacccaccca agatctggcc tccgcgccgg gttttggcgc ctcccgcggg
    10201 cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga
    10261 tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc
    10321 ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt
    10381 ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg
    10441 atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct
    10501 agttccgtcg cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca
    10561 cttggtgagt agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt
    10621 gggacggaag cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg
    10681 ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg
    10741 gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg
    10801 gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat
    10861 gggctggggc accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt
    10921 ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc acccgtacct
    10981 ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct tataatgcag
    11041 ggtggggcca cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt
    11101 cgggcctagg gtaggctctc ctgaatcgac aggcgccgga cctctggtga ggggagggat
    11161 aagtgaggcg tcagtttctt tggtcggttt tatgtaccta tcttcttaag tagctgaagc
    11221 tccggttttg aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac
    11281 cttttgaaat gtaatcattt gggtcaatat gtaattttca gtgttagact agtaaattgt
    11341 ccgctaaatt ctggccgttt ttggcttttt tgttagacga agcttggtac cgagctcgga
    11401 tctccacccc gtaccggtcc tgcaggattt aaatggcgcg ccgctagcgc taccggactc
    11461 agatctcgag ctcaagcttc gaattctgca gtcgaattca ccatgtctag actggacaag
    11521 agcaaagtca taaacggagc tctggaatta ctcaatggtg tcggtatcga aggcctgacg
    11581 acaaggaaac tcgctcaaaa gctgggagtt gagcagccta ccctgtactg gcacgtgaag
    11641 aacaagcggg ccctgctcga tgccctgcca atcgagatgc tggacaggca tcatacccac
    11701 ttctgccccc tggaaggcga gtcatggcaa gactttctgc ggaacaacgc caagtcatac
    11761 cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca
    11821 gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc
    11881 ctggagaacg cactgtacgc tctgtccgcc gtgggccact ttacactggg ctgcgtattg
    11941 gaggaacagg agcatcaagt agcaaaagag gaaagagaga cacctaccac cgattctatg
    12001 cccccacttc tgagacaagc aattgagctg ttcgaccggc agggagccga acctgccttc
    12061 cttttcggcc tggaactaat catatgtggc ctggagaaac agctaaagtg cgaaagcggc
    12121 gggccgaccg acgcccttga cgattttgac ttagacatgc tcccagccga tgcccttgac
    12181 gactttgacc ttgatatgct gcctgctgac gctcttgacg attttgacct tgacatgctc
    12241 cccgggtaac taagtaagga tccgcggccg cactagagga attccgcccc tctccccccc
    12301 ccccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
    12361 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
    12421 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
    12481 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
    12541 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
    12601 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
    12661 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
    12721 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
    12781 gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
    12841 gggacgtggt tttcctttga aaaacacgat gataagctta ccggtacgcg tgatagcttg
    12901 ccacaacccg taccaaagat ggatagatcc ggaaagcctg aactcaccgc gacgtctgtc
    12961 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc
    13021 gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat
    13081 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg
    13141 ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc
    13201 tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt
    13261 ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc
    13321 gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata
    13381 tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt
    13441 gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc
    13501 cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata
    13561 acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac
    13621 atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg
    13681 aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt
    13741 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt
    13801 cgatgcgacg caatcgtccg atccggagcc g
//
