LOCUS       sequence-436885-       13082 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible 3xFLAG-tagged NUP37.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13082)
  TITLE     Janes lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSLIK 3xFLAG-NUP37 neo
FEATURES             Location/Qualifiers
     source          1..13082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    213..801
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(266..286)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(684..695)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(804..820)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(805..821)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             1330..1510
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(1536..1553)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1542..1766
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1812..2240
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1899..1918)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2109..2130
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2249..2269)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2254..2583
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2434..2569
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2496..2515
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        2631..2678
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2697..3071
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    3201..3322
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3238..3257)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3292..3311
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3371..3387)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3371..3387)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3384..3406)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3395..3411
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3419..3425,3426..3443,3444..3449))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3464..3485
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3602..3619)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3773..4361)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3853..3872)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4532..5323,5324..5392))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5155..5174
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5393..5497)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5572..5591)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5763..6142
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6144..6342
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             6360..6540
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6587..6712
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7205..7438
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7623..7667
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7965..8082
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    8139..8163
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        8195..8509
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    8203..8221
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8239..8257
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8274..8292
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8310..8328
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8346..8364
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8381..8399
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8417..8435
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     8482..8506
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             8519..8584
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(9601..9625)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        9675..10886
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     10723..10742
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(10945..11562,11563..11568,11569..11604,11605..11607,
                     11608..11643,11644..11646,11647..11682,11683..11691)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    11744..12317
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(11911..11928)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     12138..12157
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(12333..13082,1..45)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             join(12333..13082,1..45)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(12387..12406)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     12997..13016
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgatgtac aagtaaagcg
       61 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
      121 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttta
      181 gtccctccca attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
      241 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
      301 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
      361 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
      421 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
      481 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
      541 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
      601 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
      661 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
      721 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
      781 ccctttgggc cgcctccccg catcgatacc gtcgacctcg atcgagacct agaaaaacat
      841 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
      901 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
      961 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     1021 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     1081 ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga
     1141 tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag
     1201 aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta
     1261 ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg
     1321 gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     1381 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     1441 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     1501 atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     1561 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     1621 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     1681 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     1741 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     1801 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     1861 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     1921 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     1981 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     2041 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     2101 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     2161 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     2221 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     2281 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     2341 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     2401 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     2461 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     2521 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     2581 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt
     2641 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg
     2701 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt
     2761 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg
     2821 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca
     2881 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg
     2941 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt
     3001 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg
     3061 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg
     3121 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     3181 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     3241 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     3301 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     3361 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     3421 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     3481 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     3541 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     3601 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     3661 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     3721 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     3781 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     3841 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     3901 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     3961 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     4021 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     4081 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     4141 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     4201 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     4261 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     4321 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     4381 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     4441 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     4501 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     4561 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     4621 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     4681 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     4741 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     4801 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     4861 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     4921 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     4981 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     5041 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     5101 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     5161 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     5221 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     5281 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     5341 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     5401 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     5461 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     5521 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
     5581 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
     5641 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
     5701 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
     5761 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     5821 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     5881 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     5941 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     6001 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     6061 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     6121 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     6181 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     6241 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     6301 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg
     6361 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     6421 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     6481 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     6541 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     6601 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     6661 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     6721 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     6781 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     6841 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     6901 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     6961 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     7021 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     7081 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     7141 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     7201 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     7261 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     7321 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     7381 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     7441 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     7501 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     7561 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     7621 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     7681 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     7741 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     7801 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     7861 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac
     7921 tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     7981 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     8041 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     8101 agcagagatc cagtttggtt aattctagac agttatcaac aagtttgtac aaaaaagcag
     8161 gctttaaagg aaccaattca gtcgactgga tcccgagttt actccctatc agtgatagag
     8221 aacgtatgtc gagtttactc cctatcagtg atagagaacg atgtcgagtt tactccctat
     8281 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag
     8341 tttactccct atcagtgata gagaacgtat gtcgagttta tccctatcag tgatagagaa
     8401 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgaggta ggcgtgtacg
     8461 gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcca ctagtatgga
     8521 ctacaaagac catgacggtg attataaaga tcatgacatc gattacaagg atgacgatga
     8581 caagatgaag caagatgcct caagaaatgc tgcctacact gtggattgtg aagattatgt
     8641 gcatgtggta gaatttaatc cctttgagaa tggggattca ggaaacctaa ttgcatatgg
     8701 tggcaataat tatgtggtca ttggcacgtg tacgtttcag gaagaagaag cagacgttga
     8761 aggcattcag tataaaacac ttcgaacatt tcaccatgga gtcagggttg atggcatagc
     8821 ttggagccca gagactagac ttgattcatt gcctccagta atcaaatttt gtacttcagc
     8881 tgctgatatg aaaattagat tatttacttc agatcttcag gataaaaatg aatataaggt
     8941 tttagagggc cataccgatt tcattaatgg tttggtgttt gatcccaaag aaggccaaga
     9001 aattgcaagt gtgagtgacg atcacacctg caggatttgg aacttggaag gagtgcaaac
     9061 agctcatttt gttcttcatt ctcctggcat gagtgtgtgc tggcatcctg aggagacttt
     9121 taagctaatg gttgcagaga agaatggaac aatccggttt tatgatcttt tggcccaaca
     9181 ggctatttta tctcttgaat cagaacaagt gccattaatg tcagcacact ggtgcttaaa
     9241 aaacaccttc aaagttggag ccgttgcagg aaatgattgg ttaatttggg atattactcg
     9301 gtccagttat cctcaaaata agagacctgt tcacatggat cgagcctgct tattcaggtg
     9361 gtccacaatt agtgaaaatc tgtttgcaac cactggttat cctggcaaaa tggcaagcca
     9421 gtttcaaatt catcatttag gacaccctca gcccatcctc atgggttctg tagccgttgg
     9481 atctggactg tcctggcatc gaactctccc tctgtgtgta attggaggag accacaagct
     9541 gttgttttgg gtgactgaag tataacaatt ggaagactaa tgcgttttcg agatatctag
     9601 acccagcttt cttgtacaaa gtggttgata acatctcgag ccataaagat ggttaattaa
     9661 cccacccaag atctggcctc cgcgccgggt tttggcgcct cccgcgggcg cccccctcct
     9721 cacggcgagc gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg
     9781 gacgctcagg acagcggccc gctgctcata agactcggcc ttagaacccc agtatcagca
     9841 gaaggacatt ttaggacggg acttgggtga ctctagggca ctggttttct ttccagagag
     9901 cggaacaggc gaggaaaagt agtcccttct cggcgattct gcggagggat ctccgtgggg
     9961 cggtgaacgc cgatgattat ataaggacgc gccgggtgtg gcacagctag ttccgtcgca
    10021 gccgggattt gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag
    10081 cgggctgctg ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg
    10141 tgtggagaga ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg
    10201 ggttgggggg agcgcagcaa aatggcggct gttcccgagt cttgaatgga agacgcttgt
    10261 gaggcgggct gtgaggtcgt tgaaacaagg tggggggcat ggtgggcggc aagaacccaa
    10321 ggtcttgagg ccttcgctaa tgcgggaaag ctcttattcg ggtgagatgg gctggggcac
    10381 catctgggga ccctgacgtg aagtttgtca ctgactggag aactcggttt gtcgtctgtt
    10441 gcgggggcgg cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc
    10501 gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta taatgcaggg tggggccacc
    10561 tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg ggcctagggt
    10621 aggctctcct gaatcgacag gcgccggacc tctggtgagg ggagggataa gtgaggcgtc
    10681 agtttctttg gtcggtttta tgtacctatc ttcttaagta gctgaagctc cggttttgaa
    10741 ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt
    10801 aatcatttgg gtcaatatgt aattttcagt gttagactag taaattgtcc gctaaattct
    10861 ggccgttttt ggcttttttg ttagacgaag cttggtaccg agctcggatc tccaccccgt
    10921 accggtcctg cagtcgaatt caccatgtct agactggaca agagcaaagt cataaacgga
    10981 gctctggaat tactcaatgg tgtcggtatc gaaggcctga cgacaaggaa actcgctcaa
    11041 aagctgggag ttgagcagcc taccctgtac tggcacgtga agaacaagcg ggccctgctc
    11101 gatgccctgc caatcgagat gctggacagg catcataccc acttctgccc cctggaaggc
    11161 gagtcatggc aagactttct gcggaacaac gccaagtcat accgctgtgc tctcctctca
    11221 catcgcgacg gggctaaagt gcatctcggc acccgcccaa cagagaaaca gtacgaaacc
    11281 ctggaaaatc agctcgcgtt cctgtgtcag caaggcttct ccctggagaa cgcactgtac
    11341 gctctgtccg ccgtgggcca ctttacactg ggctgcgtat tggaggaaca ggagcatcaa
    11401 gtagcaaaag aggaaagaga gacacctacc accgattcta tgcccccact tctgagacaa
    11461 gcaattgagc tgttcgaccg gcagggagcc gaacctgcct tccttttcgg cctggaacta
    11521 atcatatgtg gcctggagaa acagctaaag tgcgaaagcg gcgggccgac cgacgccctt
    11581 gacgattttg acttagacat gctcccagcc gatgcccttg acgactttga ccttgatatg
    11641 ctgcctgctg acgctcttga cgattttgac cttgacatgc tccccgggta actaagtaag
    11701 gatccgcggc cgcactagag gaattccgcc cctctccccc ccccccctct ccctcccccc
    11761 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
    11821 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
    11881 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
    11941 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
    12001 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
    12061 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
    12121 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
    12181 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
    12241 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
    12301 gaaaaacacg atgataagct taccggtcca ccatgattga acaagatgga ttgcacgcag
    12361 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
    12421 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
    12481 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
    12541 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
    12601 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
    12661 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
    12721 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
    12781 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
    12841 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg
    12901 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
    12961 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
    13021 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
    13081 at
//
