LOCUS       sequence-436888-       14207 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible V5-tagged DNM1L 
            construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14207)
  TITLE     Janes lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSLIK DNM1L-V5 neo
FEATURES             Location/Qualifiers
     source          1..14207
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2614..2638
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2670..2984
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2678..2696
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2714..2732
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2749..2767
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2785..2803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2821..2839
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2856..2874
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2892..2910
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2957..2981
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5121..5162
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(5201..5225)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5275..6486
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6323..6342
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(6545..7162,7163..7168,7169..7204,7205..7207,7208..7243,
                     7244..7246,7247..7282,7283..7291)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    7344..7917
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7511..7528)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7738..7757
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7933..8727
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7933..8727
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7987..8006)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8597..8616
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    8895..9483
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8948..8968)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9366..9377)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9486..9502)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9487..9503)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10012..10192
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10218..10235)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10224..10448
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10494..10922
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10581..10600)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10791..10812
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10931..10951)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10936..11265
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11116..11251
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11178..11197
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11313..11360
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11379..11753
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11883..12004
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11920..11939)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11974..11993
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12053..12069)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12053..12069)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12066..12088)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12077..12093
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12101..12107,12108..12125,12126..12131))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12146..12167
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12284..12301)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12455..13043)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12535..12554)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13214..14005,14006..14074))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13837..13856
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14075..14179)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaattc tagacagtta tcaacaagtt tgtacaaaaa agcaggcttt
     2641 aaaggaacca attcagtcga ctggatcccg agtttactcc ctatcagtga tagagaacgt
     2701 atgtcgagtt tactccctat cagtgataga gaacgatgtc gagtttactc cctatcagtg
     2761 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
     2821 tccctatcag tgatagagaa cgtatgtcga gtttatccct atcagtgata gagaacgtat
     2881 gtcgagttta ctccctatca gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg
     2941 aggcctatat aagcagagct cgtttagtga accgtcagat cgccactagt atggaggcgc
     3001 taattcctgt cataaacaag ctccaggacg tcttcaacac ggtgggcgcc gacatcatcc
     3061 agctgcctca aatcgtcgta gtgggaacgc agagcagcgg aaagagctca gtgctagaaa
     3121 gcctggtggg gagggacctg cttcccagag gtactggaat tgtcacccgg agacctctca
     3181 ttctgcaact ggtccatgtt tcacaagaag ataaacggaa aacaacagga gaagaaaatg
     3241 gggtggaagc agaagaatgg ggtaaatttc ttcacaccaa aaataagctt tacacggatt
     3301 ttgatgaaat tcgacaagaa attgaaaatg aaacagaaag aatttcagga aataataagg
     3361 gagtaagccc tgaaccaatt catcttaaga ttttttcacc caacgttgtc aatttgacac
     3421 ttgtggattt gccaggaatg accaaggtgc ctgtaggtga tcaacctaag gatattgagc
     3481 ttcaaatcag agagctcatt cttcggttca tcagtaatcc taattccatt atcctcgctg
     3541 tcactgctgc taatacagat atggcaacat cagaggcact taaaatttca agagaggtag
     3601 atccagatgg tcgcagaacc ctagctgtaa tcactaaact tgatctcatg gatgcgggta
     3661 ctgatgccat ggatgtattg atgggaaggg ttattccagt caaacttgga ataattggag
     3721 tagttaacag gagccagcta gatattaaca acaagaagag tgtaactgat tcaatccgtg
     3781 atgagtatgc ttttcttcaa aagaaatatc catctctggc caatagaaat ggaacaaagt
     3841 atcttgctag gactctaaac aggttactga tgcatcacat cagagattgt ttaccagagt
     3901 tgaaaacaag aataaatgtt ctagctgctc agtatcagtc tcttctaaat agctacggtg
     3961 aacccgtgga tgataaaagt gctactttac tccaacttat taccaaattt gccacagaat
     4021 attgtaacac tattgaagga actgcaaaat atattgaaac ttcggagcta tgcggtggtg
     4081 ctagaatttg ttatattttc catgagactt ttgggcgaac cttagaatct gttgatccac
     4141 ttggtggcct taacactatt gacattttga ctgccattag aaatgctact ggtcctcgtc
     4201 ctgctttatt tgtgcctgag gtttcatttg agttactggt gaagcggcaa atcaaacgtc
     4261 tagaagagcc cagcctccgc tgtgtggaac tggttcatga ggaaatgcaa aggatcattc
     4321 agcactgtag caattacagt acacaggaat tgttacgatt tcctaaactt catgatgcca
     4381 tagttgaagt ggtgacttgt cttcttcgta aaaggttgcc tgttacaaat gaaatggtcc
     4441 ataacttagt ggcaattgaa ctggcttata tcaacacaaa acatccagac tttgctgatg
     4501 cttgtgggct aatgaacaat aatatagagg aacaaaggag aaacaggcta gccagagaat
     4561 taccttcagc tgtatcacga gacaagttaa ttcaggacag cagaagagaa actaaaaatg
     4621 ttgcatctgg aggtggtggg gttggagatg gtgttcaaga accaaccaca ggcaactgga
     4681 gaggaatgct gaaaacttca aaagctgaag agttattagc agaagaaaaa tcaaaaccca
     4741 ttccaattat gccagccagt ccacaaaaag gtcatgccgt gaacctgcta gatgtgccag
     4801 ttcctgttgc acgaaaacta tctgctcggg aacagcgaga ttgtgaggtt attgaacgac
     4861 tcattaaatc atattttctc attgtcagaa agaatattca agacagtgtg ccaaaggcag
     4921 taatgcattt tttggttaat catgtgaaag acactcttca gagtgagcta gtaggccagc
     4981 tgtataaatc atccttattg gatgatcttc tgacagaatc tgaggacatg gcacagcgca
     5041 ggaaagaagc agctgatatg ctaaaggcat tacaaggagc cagtcaaatt attgctgaaa
     5101 tccgggagac tcatctttgg ggtaagccta tccctaaccc tctcctcggt ctcgattcta
     5161 cgtgagaatt ggaagactaa tgcgttttcg agatatctag acccagcttt cttgtacaaa
     5221 gtggttgata acatctcgag ccataaagat ggttaattaa cccacccaag atctggcctc
     5281 cgcgccgggt tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt
     5341 cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc
     5401 gctgctcata agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg
     5461 acttgggtga ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt
     5521 agtcccttct cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat
     5581 ataaggacgc gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt
     5641 tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag cgggctgctg ggctggccgg
     5701 ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg
     5761 ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg ggttgggggg agcgcagcaa
     5821 aatggcggct gttcccgagt cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt
     5881 tgaaacaagg tggggggcat ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa
     5941 tgcgggaaag ctcttattcg ggtgagatgg gctggggcac catctgggga ccctgacgtg
     6001 aagtttgtca ctgactggag aactcggttt gtcgtctgtt gcgggggcgg cagttatggc
     6061 ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg
     6121 tcacccgttc tgttggctta taatgcaggg tggggccacc tgccggtagg tgtgcggtag
     6181 gcttttctcc gtcgcaggac gcagggttcg ggcctagggt aggctctcct gaatcgacag
     6241 gcgccggacc tctggtgagg ggagggataa gtgaggcgtc agtttctttg gtcggtttta
     6301 tgtacctatc ttcttaagta gctgaagctc cggttttgaa ctatgcgctc ggggttggcg
     6361 agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt
     6421 aattttcagt gttagactag taaattgtcc gctaaattct ggccgttttt ggcttttttg
     6481 ttagacgaag cttggtaccg agctcggatc tccaccccgt accggtcctg cagtcgaatt
     6541 caccatgtct agactggaca agagcaaagt cataaacgga gctctggaat tactcaatgg
     6601 tgtcggtatc gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc
     6661 taccctgtac tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat
     6721 gctggacagg catcataccc acttctgccc cctggaaggc gagtcatggc aagactttct
     6781 gcggaacaac gccaagtcat accgctgtgc tctcctctca catcgcgacg gggctaaagt
     6841 gcatctcggc acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt
     6901 cctgtgtcag caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca
     6961 ctttacactg ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga
     7021 gacacctacc accgattcta tgcccccact tctgagacaa gcaattgagc tgttcgaccg
     7081 gcagggagcc gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa
     7141 acagctaaag tgcgaaagcg gcgggccgac cgacgccctt gacgattttg acttagacat
     7201 gctcccagcc gatgcccttg acgactttga ccttgatatg ctgcctgctg acgctcttga
     7261 cgattttgac cttgacatgc tccccgggta actaagtaag gatccgcggc cgcactagag
     7321 gaattccgcc cctctccccc ccccccctct ccctcccccc cccctaacgt tactggccga
     7381 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     7441 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     7501 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     7561 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     7621 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     7681 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     7741 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     7801 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
     7861 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagct
     7921 taccggtcca ccatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     7981 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     8041 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     8101 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     8161 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     8221 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     8281 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     8341 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     8401 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     8461 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     8521 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     8581 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     8641 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     8701 tatcgccttc ttgacgagtt cttctgatgt acaagtaaag cggccgcgac tctagatcat
     8761 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     8821 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt tagtccctcc caattcgata
     8881 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     8941 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     9001 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     9061 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     9121 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     9181 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     9241 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     9301 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     9361 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     9421 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     9481 cgcatcgata ccgtcgacct cgatcgagac ctagaaaaac atggagcaat cacaagtagc
     9541 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     9601 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     9661 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     9721 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac
     9781 tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta
     9841 ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac
     9901 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac
     9961 agccgcctag catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc
    10021 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
    10081 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
    10141 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt
    10201 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
    10261 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
    10321 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
    10381 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
    10441 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
    10501 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
    10561 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
    10621 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
    10681 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
    10741 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
    10801 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
    10861 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
    10921 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
    10981 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
    11041 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
    11101 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
    11161 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
    11221 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
    11281 tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata
    11341 tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg
    11401 ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg
    11461 ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc
    11521 tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg
    11581 tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg
    11641 acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc
    11701 tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc
    11761 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
    11821 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
    11881 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    11941 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    12001 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
    12061 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
    12121 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
    12181 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
    12241 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
    12301 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
    12361 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    12421 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    12481 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
    12541 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    12601 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    12661 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    12721 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    12781 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    12841 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
    12901 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    12961 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    13021 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    13081 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
    13141 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
    13201 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
    13261 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
    13321 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
    13381 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    13441 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    13501 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    13561 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    13621 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    13681 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    13741 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    13801 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    13861 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    13921 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    13981 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    14041 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    14101 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    14161 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgac
//
