LOCUS       sequence-436909-        4175 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Native rat lactase gene (truncated) as an autologous marker (X-Gal, 
            X-Fuc).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4175)
  TITLE     Gene Tracking
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: icLCT
FEATURES             Location/Qualifiers
     source          1..4175
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    18..89
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     regulatory      696..705
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3841..3962
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3852..3871)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3906..3925
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    4090..4161
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
ORIGIN
        1 aggcctacat ctagatacat cttggaccat tagctccaca ggtatcttct tccctctagt
       61 ggtcataaca gcagcttcag ctacctctca atcacaacct aggacatgtt tacataactt
      121 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      181 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      241 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      301 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      361 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat gaattggttt
      421 gatctgatta taactagtgg agaagagcat gcttgagggc tgagtgcccc tcagtgggca
      481 gagagcacat ggcccacagt ccctgagaag ttggggggag gggtgggcaa ttgaactggt
      541 gcctagagaa ggtggggctt gggtaaactg ggaaagtgat gtggtgtact ggctccacct
      601 ttttccccag ggtgggggag aaccatatat aagtgcagta gtctctgtga acattcgcta
      661 gcagagacct aagctggctc tcgagaatag ctgcagccac catgggattc tcagccaaga
      721 aagtcaagag aaacccactg cctgtgagag cagacttcac ctccagagcc agggtcactg
      781 acagccttcc atctgaggtg ccctccaagg ctaagatagt gtgggagaag ttctccaagc
      841 agcccaggtt tgaaagagac ttgttctatc atggcacatt cagagatgac ttcctgtggg
      901 gtgtatcctc ttcagcctat cagattgaag gaggatggaa tgctgatggc aaaggtccca
      961 gcatctggga taacttcacc cacactccag ggaatggtgt gaaagacaat gccactggag
     1021 atgtagcttg tgacagctat caccaactgg atgctgacct aaacatcctt agaaccctga
     1081 aggtcaagtc ctatagattc tccatctcct ggtccagaat cttcccaaca ggaagaaaca
     1141 gtaccatcaa caaacaaggt gttgattatt acaacagact gattgacagc ttggtggaca
     1201 acaacatctt ccctatggtg acactgtttc actgggacct gcctcaggcc ctccaggata
     1261 ttggaggttg ggagaaccct tctttgattg agttgtttga cagctatgca gacttctgct
     1321 ttaagacctt tggtgacaga gtcaagttct ggatgacctt taatgagccc tggtgtcatg
     1381 tggtgttagg ctacagttca gggatatttc ctccaagtgt tcaggagcca ggctggctac
     1441 cttataaggt tagccatatt gtcattaagg cacatgctag ggtctaccat acttatgatg
     1501 agaagtacag gtctgagcag aaaggtgtca tctctctcag cctcaacact cactgggcag
     1561 aacccaagga cccagggctc caaagggatg tggaagctgc tgacagaatg ctgcagttta
     1621 ctatgggctg gtttgcccac cctatcttca agaatggtga ctatccagat gtgatgaaat
     1681 ggactgtggg taacaggagt gagctgcagc acctggccag ctccagactg cctaccttca
     1741 cagaggaaga gaagaattat gtcagaggca ctgctgatgt cttctgcatc aacacctaca
     1801 cttctgtgtt tgtgcagcac agcactccca ggctgaaccc acccagctat gatgatgaca
     1861 tggagttgaa gctaattgaa atgaactctt ctactggtgt gatgcaccca gatgtgccct
     1921 ggggtactag gaggctgctg aactggatca aggaggagta tggcaacatt cctatttata
     1981 tcactgagaa tggacaggga ctggagaatc ccacactgga tgacacagaa aggatcttct
     2041 accacaagac ctatatcaat gaggctctga aagcctacaa gctggatggt gtggacctta
     2101 gagggtactc tgcctggact ctgatggatg actttgagtg gctgcttggc tacaccatga
     2161 gatttggatt gtatcatgtt gactttaatc atgtgagcag acctagaaca gcaagagcct
     2221 cagccagata ctatgcagag gtcattgcca acaatggtat gcctctggcc agggaagatg
     2281 agttcctgta tggagagttt cccaagggct tcatctggag tgctgcttct gcttcatatc
     2341 aggttgaagg tgcttggaga gcagatggca aaggactcag tatctgggac acattctccc
     2401 acacaccact gaggattggg aatgatgaca atggagatgt ggcatgtgac agttaccaca
     2461 agattgctga ggatgtggtg gcccttcaga acctgggtgt gtcccactat agattctcca
     2521 ttgcctggtc cagaatcctc ccagatggaa ccaccaagtt catcaatgaa gctggcctca
     2581 gttactatgt tagattcatt gatgccctgc tggctgctgg catcacacca caggtgacta
     2641 tttatcactg ggacctgcca caggcactgc aagatgtggg tggctgggag aatgagacta
     2701 ttgtgcagag atttaaggag tatgctgatg ttctcttcca gaggctgggt gacagagtca
     2761 agttctggat cactctgaat gagccctttg tcattgcagc ccaaggctat ggcactggtg
     2821 tgtctgctcc aggaatctcc ttcagacctg gcacagctcc ttacattgct ggacacaacc
     2881 taataaaggc ccatgctgaa gcctggcatc tctacaatga tgtatacaga gccagacagg
     2941 gaggcaccat ctccatcact atcagcagtg actgggctga acccagagat cccaccaacc
     3001 agggagatgt ggaggcagcc aggagatatg tacagtttat gggtggctgg tttgctcacc
     3061 ctatcttcaa gaatggagat taccctgagg tgatgaagac aagaatcaga gacagaagcc
     3121 tggctgctgg cctcaacaag tccaggctcc ctgagtttac tgagagtgag aagagtagga
     3181 tcaagggcac ctttgacttc tttggtttca accactacac cactgtccta gcttacaacc
     3241 ttgactaccc tgctgccttc tcctcctttg atgcagacag gggtgttgct tccattgcag
     3301 acagctcttg gccagtctct ggctccttct ggctgaaggt gactccattt ggcttcagaa
     3361 ggatcttgaa ctggctgaag gaggagtaca ataatcctcc tatttatgtc actgagaatg
     3421 gtgtctccag aagaggagag cctgaactca atgacactga caggatctac tacctgagaa
     3481 gctacatcaa tgaagccctc aaggctgtac aggacaaggt ggaccttaga ggctacactg
     3541 tatggagcat catggacaac tttgaatggg ccacaggctt tgcagagagg tttggtgtgc
     3601 actttgtgaa caggtctgac ccatctttgc caaggattcc caaggcatca gccaagttct
     3661 atgccaccat tgtcagatgc aatggctttc ctgaccctgc acaaggacct catccttgtc
     3721 tccagcaacc agaagatgct gcacccactg ccagtcctgt gcagtctgaa gtgcccttcc
     3781 tgggtctgat gctgggcatt gcagaagcac agacagctct gtaaagcttg gatcccatga
     3841 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     3901 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     3961 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     4021 tttaaagcaa gtaaaacctc tacaaatgtg gtaaaggaat tcaattccat ctctcttaag
     4081 gtagccagac attcctttaa tggtcttttc tggacaccac taggggtcag aagtagttca
     4141 tcaaactttc ttccctccct aatctatcag atatc
//
