LOCUS       sequence-43692-e         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbB2k-GFP
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        39..94
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    40..58
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(186..205)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 tatgtatatc tccttcttaa aagatctttt gaattctttt ctctatcact gatagggagt
       61 ggtaaaataa ctctatcaac gatagagtgt caacaaaaat taggaattaa tgatgtctag
      121 attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg tcggaatcga
      181 aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta cattgtattg
      241 gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt tagataggca
      301 ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac gtaataacgc
      361 taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac atttaggtac
      421 acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt tatgccaaca
      481 aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt ttactttagg
      541 ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa cacctactac
      601 tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc aaggtgcaga
      661 gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac aactt
//
