LOCUS       sequence-436940-       10451 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral backbone with EBFP2 reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10451)
  AUTHORS   Werder RB, Liu T, Abo KM, Lindstrom-Vautrin J, Villacorta-Martin C, 
            Huang J, Hinds A, Boyer N, Bullitt E, Liesa M, Silverman EK, Kotton 
            DN, Cho MH, Zhou X, Wilson AA
  TITLE     CRISPR interference interrogation of COPD GWAS genes reveals the 
            functional significance of desmoplakin in iPSC-derived alveolar 
            epithelial cells.
  JOURNAL   Sci Adv. 2022 Jul 15;8(28):eabo6566. doi: 10.1126/sciadv.abo6566. 
            Epub 2022 Jul 13.
  PUBMED    35857525
REFERENCE   2  (bases 1 to 10451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2022-07-15"; volume: "8(28):eabo6566. doi"; pages: " 
            10.1126/sciadv.abo6566. Epub 2022 Jul 13"
COMMENT     Sequence Label: pLV-GG-hUBC-EBFP2
FEATURES             Location/Qualifiers
     source          1..10451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             9..189
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(207..405)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(232..252)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        407..786
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     958..977
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1052..1156
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1157..1225,1226..2017)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1375..1394)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2188..2776
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2677..2696
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2930..2947
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3064..3085
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3100..3105,3106..3123,3124..3130)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3138..3154
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3162..3178
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    3227..3348
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3238..3257)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3292..3311
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(3478..3852)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(3871..3918)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(3966..4295)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3980..4115
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4034..4053)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     4280..4300
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(4309..4737)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4419..4440)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4631..4650
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    4783..5007
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4996..5013
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             5039..5219
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     5724..5740
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5725..5741
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5744..6332
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5850..5861
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6259..6279
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(6357..7070,7071..7073,7074..7076))
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     7010..7031
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      7073..7082
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7129..8340)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(7273..7292)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    8417..8438
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8453..8458,8459..8476,8477..8483)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8491..8507
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8515..8531
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             8527..8853
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGATTVSY
                     HNFV"
     primer_bind     8550..8570
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8552..8570
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8617..8636)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8618..8636)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8646..8663)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8646..8662)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8655..8677)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     protein_bind    8840..8873
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    8918..9035
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(9333..9377)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9562..9795
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    10288..10413
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 ggcgccactg ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc
       61 agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg
      121 cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca
      181 gagagaccca gtacaggcaa aacgcgctgc ttatatagac ctcccaccgt acacgcctac
      241 cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt
      301 ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt
      361 caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga
      421 tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat
      481 aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat
      541 acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat
      601 ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg
      661 ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca
      721 tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta gtcaataatc
      781 aatgtcaacg cgtatatctg gcccgtacat cgcgaagcag cgcaaaacgc ctaaccctaa
      841 gcagattctt catgcaattg tcggtcaagc cttgccttgt tgtagcttaa attttgctcg
      901 cgcactactc agcgacctcc aacacacaag cagggagcag atactggctt aactatgcgg
      961 catcagagca gattgtactg agagtgcacc ataggggatc gggagatctc ccgatccgtc
     1021 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     1081 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     1141 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     1201 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     1261 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     1321 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     1381 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     1441 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     1501 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     1561 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     1621 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     1681 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     1741 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     1801 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     1861 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     1921 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     1981 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     2041 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     2101 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     2161 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2221 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2281 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     2341 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2401 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2461 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2521 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2581 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2641 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2701 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2761 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2821 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     2881 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     2941 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     3001 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     3061 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     3121 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     3181 tgattacgcc aagctctagc tagaggtcga cggtatacag acatgataag atacattgat
     3241 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     3301 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttgg ggtgggcgaa
     3361 gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc ggaaaacgat
     3421 tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgta gcacgtgtca
     3481 gtcctgctcc tcggccacga agtgcacgca gttgccggcc gggtcgcgca gggcgaactc
     3541 ccgcccccac ggctgctcgc cgatctcggt catggccggc ccggaggcgt cccggaagtt
     3601 cgtggacacg acctccgacc actcggcgta cagctcgtcc aggccgcgca cccacaccca
     3661 ggccagggtg ttgtccggca ccacctggtc ctggaccgcg ctgatgaaca gggtcacgtc
     3721 gtcccggacc acaccggcga agtcgtcctc cacgaagtcc cgggagaacc cgagccggtc
     3781 ggtccagaac tcgaccgctc cggcgacgtc gcgcgcggtg agcaccggaa cggcactggt
     3841 caacttggcc atggtttagt tcctcacctt gtcgtattat actatgccga tatactatgc
     3901 cgatgattaa ttgtcaacac gtgctgatca gatccgaaaa tggatataca agctcccggg
     3961 agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca
     4021 gaggcagagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga
     4081 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg
     4141 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
     4201 tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
     4261 gagcctgggg actttccaca ccctaactga cacacattcc acagaattaa ttcgcgttaa
     4321 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     4381 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     4441 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     4501 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     4561 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     4621 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     4681 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtggg
     4741 gataccccct agagccccag ctggttcttt ccgcctcaga agccatagag cccaccgcat
     4801 ccccagcatg cctgctattg tcttcccaat cctccccctt gctgtcctgc cccaccccac
     4861 cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca ttttattagg
     4921 aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag gggcaaacaa
     4981 cagatggctg gcaactagaa ggcacagtcg aggctgatca gcgggtttaa acgggccctg
     5041 ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac
     5101 aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt
     5161 gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca
     5221 gtacagtccg gatgcagctc tcgggccatg tgatgaaatg ctaggcggct gtcaaacctc
     5281 cactctaata cttctctctc cgggtcatcc atcccatgca ggctcacagg gtgtaacaag
     5341 cgggtgttct ctccttcatt ggcttcttct accttctctt gctcaactgg tactagcttg
     5401 tagcaccatc caaaggtcag tggatatctg atccctggcc ctggtgtgta gttctgccaa
     5461 tcagggaagt agccttgtgt gtggtagatc cacagatcaa ggatatcttg tcttcgttgg
     5521 gagtgaatta gcccttccag tccccccttt tcttttaaaa agtggctaag atctacagct
     5581 gccttgtaag tcattggtct taaaggtacc tgaggtgtga ctggaaaacc cacctcctcc
     5641 tcctcttgtg cttctagcca ggcacaatca gcattggtag ctgctgtatt gctacttgtg
     5701 attgctccat gtttttctag gtctcgaggt cgacggtatc gatgcgggga ggcggcccaa
     5761 agggagatcc gactcgtctg agggcgaagg cgaagacgcg gaagaggccg cagagccggc
     5821 agcaggccgc gggaaggaag gtccgctgga ttgagggccg aagggacgta gcagaaggac
     5881 gtcccgcgca gaatccaggt ggcaacacag gcgagcagcc aaggaaagga cgatgatttc
     5941 cccgacaaca ccacggaatt gtcagtgccc aacagccgag cccctgtcca gcagcgggca
     6001 aggcaggcgg cgatgagttc cgccgtggca atagggaggg ggaaagcgaa agtcccggaa
     6061 aggagctgac aggtggtggc aatgccccaa ccagtggggg ttgcgtcagc aaacacagtg
     6121 cacaccacgc cacgttgcct gacaacgggc cacaactcct cataaagaga cagcaaccag
     6181 gatttataca aggaggagaa aatgaaagcc atacgggaag caatagcatg atacaaaggc
     6241 attaaagcag cgtatccaca tagcgtaaaa ggagcaacat agttaagaat accagtcaat
     6301 ctttcacaaa ttttgtaatc cagaggttga ttatcgataa gcttgatatc gaattcttac
     6361 ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tgcggaactc cagcaggacc
     6421 atgtgatcgc gcttctcgtt ggggtctttg ctcagcacgg actgggtgct caggtagtgg
     6481 ctgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg
     6541 agctgcacgc tgccgtcctc cacgttgtgg cggatcttga agttcacctt gatgccgttc
     6601 ttctgcttga cggccatgat atagatgttg tggctgttga agttgtactc cagcttgtgc
     6661 cccaggatgt tgccgtcctc cttgaagtcg acgcccttca gctcgatgcg gttcaccagg
     6721 gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagg tgccgtcgtc cttgaagaag
     6781 atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc
     6841 atgtggtcgg ggtagcgggc gaagcactgc acgccgtggc tcagggtggt cacgagggtg
     6901 ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgttg
     6961 gtggcatcgc cctcgccctc gcccctcacg ctgaacttgt ggccgtttac gtcgccgtcc
     7021 agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct caccatggtg
     7081 gcaccggtac ccggggatcc tctagagtcg acctgcagcc caagcttcgt ctaacaaaaa
     7141 agccaaaaac ggccagaatt tagcggacaa tttactagtc taacactgaa aattacatat
     7201 tgacccaaat gattacattt caaaaggtgc ctaaaaaact tcacaaaaca cactcgccaa
     7261 ccccgagcgc atagttcaaa accggagctt cagctactta agaagatagg tacataaaac
     7321 cgaccaaaga aactgacgcc tcacttatcc ctcccctcac cagaggtccg gcgcctgtcg
     7381 attcaggaga gcctacccta ggcccgaacc ctgcgtcctg cgacggagaa aagcctaccg
     7441 cacacctacc ggcaggtggc cccaccctgc attataagcc aacagaacgg gtgacgtcac
     7501 gacacgacga gggcgcgcgc tcccaaaggt acgggtgcac tgcccaacgg caccgccata
     7561 actgccgccc ccgcaacaga cgacaaaccg agttctccag tcagtgacaa acttcacgtc
     7621 agggtcccca gatggtgccc cagcccatct cacccgaata agagctttcc cgcattagcg
     7681 aaggcctcaa gaccttgggt tcttgccgcc caccatgccc cccaccttgt ttcaacgacc
     7741 tcacagcccg cctcacaagc gtcttccatt caagactcgg gaacagccgc cattttgctg
     7801 cgctcccccc aacccccagt tcagggcaac cttgctcgcg gacccagact acagcccttg
     7861 gcggtctctc cacacgcttc cgtcccaccg agcggcccgg cggccacgaa agccccggcc
     7921 agcccagcag cccgctactc accaagtgac gatcacagcg atccacaaac aagaaccgcg
     7981 acccaaatcc cggctgcgac ggaactagct gtgccacacc cggcgcgtcc ttatataatc
     8041 atcggcgttc accgccccac ggagatccct ccgcagaatc gccgagaagg gactactttt
     8101 cctcgcctgt tccgctctct ggaaagaaaa ccagtgccct agagtcaccc aagtcccgtc
     8161 ctaaaatgtc cttctgctga tactggggtt ctaaggccga gtcttatgag cagcgggccg
     8221 ctgtcctgag cgtccgggcg gaaggatcag gacgctcgct gcgcccttcg tctgacgtgg
     8281 cagcgctcgc cgtgaggagg ggggcgcccg cgggaggcgc caaaacccgg cgcggaggcc
     8341 agatctggag ccgacacggg ttaattaatt gtggagacgt catgcagtgg aaagcgggca
     8401 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     8461 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     8521 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     8581 tggaggacct cgaggggggg cccggtaccc aattcgccct atagtgagtc gtattacgcg
     8641 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     8701 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     8761 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     8821 gcaaccaccg tctcttatca taacttcgta taatgtatgc tatacgaagt tatttaatta
     8881 accaaactgg atctctgctg tccctgtaat aaacccgaaa attttgaatt tttgtaattt
     8941 gtttttgtaa ttctttagtt tgtatgtctg ttgctattat gtctactatt ctttcccctg
     9001 cactgtaccc cccaatcccc ccttttcttt taaaattgtg gatgaatact gccatttgtc
     9061 tgcagaattg gcgcacgcag tgccgatccg ttcactaatc gaatggatct gtctctgtct
     9121 ctctctccac cttcttcttc tattccttcg ggcctgtcgg gtcccctcgg ggttgggagg
     9181 tgggtctgaa acgataatgg tgaatatccc tgcctaactc tattcactat agaaagtaca
     9241 gcaaaaacta ttcttaaacc taccaagcct cctactatca ttatgaataa ttttatatac
     9301 cacagccaat ttgttatgtt aaaccaattc cacaaacttg cccatttatc taattccaat
     9361 aattcttgtt cattcttttc ttgctggttt tgcgattctt caattaagga gtgtattaag
     9421 cttgtgtaat tgttaatttc tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt
     9481 tccagagatt tattactcca actagcattc caaggcacag cagtggtgca aatgagtttt
     9541 ccagagcaac cccaaatccc caggagctgt tgatccttta ggtatctttc cacagccagg
     9601 attcttgcct ggagctgctt gatgccccag actgtgagtt gcaacagatg ctgttgcgcc
     9661 tcaatagccc tcagcaaatt gttctgctgc tgcactatac cagacaataa ttgtctggcc
     9721 tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc ctgctgctcc caagaaccca
     9781 aggaacaaag ctcctattcc cactgctctt ttttctctct gcaccactct tctctttgcc
     9841 ttggtgggtg ctactcctaa tggttcaatt tttactactt tatatttata taattcactt
     9901 ctccaattgt ccctcatatc tcctcctcca ggtctgaaga tcagcggccg cttgctgtgc
     9961 ggtggtctta cttttgtttt gctcttcctc tatcttgtct aaagcttcct tggtgtcttt
    10021 tatctctatc ctttgatgca cacaatagag ggttgctact gtattatata atgatctaag
    10081 ttcttctgat cctgtctgaa gggatggttg tagctgtccc agtatttgtc tacagccttc
    10141 tgatgtttct aacaggccag gattaactgc gaatcgttct agctccctgc ttgcccatac
    10201 tatatgtttt aatttatatt ttttctttcc ccctggcctt aaccgaattt tttcccatcg
    10261 cgatctaatt ctcccccgct taatactgac gctctcgcac ccatctctct ccttctagcc
    10321 tccgctagtc aaaatttttg gcgtactcac cagtcgccgc ccctcgcctc ttgccgtgcg
    10381 cgcttcagca agccgagtcc tgcgtcgaga gagctcctct ggtttccctt tcgctttcaa
    10441 gtccctgttc g
//
