LOCUS       sequence-436964-       10207 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of gRNAs under U6T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10207)
  AUTHORS   Binan L, Jiang A, Danquah SA, Valakh V, Simonton B, Bezney J, 
            Manguso RT, Yates KB, Nehme R, Cleary B, Farhi SL
  TITLE     Simultaneous CRISPR screening and spatial transcriptomics reveal 
            intracellular, intercellular, and functional transcriptional 
            circuits.
  JOURNAL   Cell. 2025 Mar 10:S0092-8674(25)00197-7. doi: 
            10.1016/j.cell.2025.02.012.
  PUBMED    40081369
REFERENCE   2  (bases 1 to 10207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            Mar 10:S0092-8674(25)00197-7. doi: 10.1016/j.cell.2025.02.012."
COMMENT     Sequence Label: Perturb-FISH
FEATURES             Location/Qualifiers
     source          1..10207
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     428..448
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    547..1135
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(600..620)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1018..1029)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1207..1440
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1518..1639
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1555..1574)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1609..1628
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1679..1814
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1863..1880)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1863..1879)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1872..1894)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2021..2476
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2108..2127)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2318..2339
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2502..2606
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2607..2675,2676..3467)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2825..2844)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3638..4226
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4127..4146
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4380..4397
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4514..4535
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4550..4555,4556..4573,4574..4580)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4588..4604
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4593..4615
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4612..4628
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4612..4628
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4647..4667
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4649..4667
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4695..4921
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4922..5102
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5149..5274
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5767..6000
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6185..6229
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     6407..6427
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6578..6597
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        6639..6657
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    8692..8809
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8948..10126
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          9179..10117
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     10074..10094
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(10139..10207,1..531)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10139..10158)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
       61 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
      121 gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga ccacgccgga gagcgtcgaa
      181 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
      241 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
      301 ttcctggcca ccgtcggagt ctcgcccgac caccagggca agggtctggg cagcgccgtc
      361 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
      421 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
      481 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aacgcgttaa
      541 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
      601 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
      661 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
      721 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
      781 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
      841 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
      901 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
      961 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     1021 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     1081 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     1141 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     1201 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     1261 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     1321 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     1381 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     1441 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     1501 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     1561 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     1621 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     1681 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     1741 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     1801 cttttttgga ggcctaggga cgtacccaat tcgcccgcta gtgagtcgta ttacgcgcgc
     1861 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     1921 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     1981 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     2041 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     2101 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     2161 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     2221 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     2281 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     2341 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     2401 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     2461 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     2521 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     2581 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     2641 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     2701 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     2761 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     2821 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     2881 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     2941 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     3001 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     3061 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     3121 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     3181 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     3241 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     3301 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     3361 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     3421 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     3481 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     3541 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     3601 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3661 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3721 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3781 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3841 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3901 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3961 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     4021 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     4081 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     4141 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     4201 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     4261 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     4321 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     4381 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     4441 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     4501 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     4561 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     4621 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     4681 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     4741 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     4801 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     4861 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     4921 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     4981 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     5041 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     5101 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     5161 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     5221 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     5281 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     5341 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     5401 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     5461 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     5521 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     5581 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     5641 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     5701 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     5761 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     5821 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     5881 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     5941 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     6001 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     6061 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     6121 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     6181 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     6241 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     6301 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     6361 accattatcg tttcagaccc acctcccaac cccgagggga cccagagagg gcctatttcc
     6421 catgattcct tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa
     6481 tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct
     6541 tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac
     6601 ttgaaagtat ttcgatttct tggctttata tatcttgcta atacgactca ctataggaga
     6661 cggttgtaaa tgagcacaca aaatacacat gctaaaatat tatattctat gacctttata
     6721 aaatcaacca aaatcttctt tttaataact ttagtatcaa taattagaat ttttatgttc
     6781 ctttttgcaa acttttaata aaaatgagca aaataaaaaa acgctagttt tagtaactcg
     6841 cgttgttttc ttcaccttta ataatagcta ctccaccact tgttcctaag cggtcagctc
     6901 ctgcttcaat cattttttga gcatcttcaa atgttctaac tccaccagct gctttaacta
     6961 aagcattgtc tttaacaact gacttcatta gtttaacatc ttcaaatgtt gcacctgatt
     7021 ttgaaaatcc tgttgatgtt ttaacaaatt ctaatccagc ttcaacagct atttcacaag
     7081 ctttcatgat ttcttctttt gttaataaac aattttccat aatacattta acaacatgtg
     7141 atccagctgc tttttttaca gctttcatgt cttctaaaac taattcataa tttttgtctt
     7201 ttaatgcacc aatatttaat accatatcaa tttctgttgc accatcttta attgcttcag
     7261 aaacttcgaa tgcttttgta gctgttgtgc atgcacctag aggaaaacct acaacatttg
     7321 ttattcctac atttgtgcct tttaataatt ctttacaata gcttgttcaa tatgaattaa
     7381 cacaaactgt tgcaaaatca aattcaattg cttcatcaca taattgttta atttcagctt
     7441 tcgtagcatc ttgttttaat aatgtgtgat ctatatattt gtttagtttc attttttctc
     7501 ctatatattc atttttaatt ttaattcttt aataatttcg tctactttaa ctttagcgtt
     7561 ttgaacagat tcaccaacac ctataaaata aatttttagt ttaggttcag ttccacttgg
     7621 gcgaacagca aatcatgact tatcttctaa ataaaatttt agtaagtctt gtcctggcat
     7681 attatacatt ccatcgatgt agtcttcaac attaacaact ttaagtccag caatttgagt
     7741 taagggtgtt gctctcaatg atttcattaa tggttcaatt tttaatttct tttcttctgg
     7801 tttaaaattc aagtttaaag tgaaagtgta atatgcaccc atttctttaa ataaatcttc
     7861 taaatagtct actaatgttt tattttgttt tttataaaat caagcagcct ctgctattaa
     7921 tatagaagct tgtattccat ctttatctct agctgagtca tcaattacat atccataact
     7981 ttcttcataa gcaaaaacaa aatttaatcc gttatcttct tctttagcaa tttctctacc
     8041 cattcattta aatccagtta aagtttttac aatattaact ccatattttt catgagcgat
     8101 tctatcaccc aaatcacttg ttacaaaact tgaatataga gccggatttt ttggaatgct
     8161 atttaagcgt tttagatttg ataattttca atcaattaaa attggtcctg tttgatttcc
     8221 atctaatctt acaaaatgac catcatgttt tattgccatt ccaaatctgt cagcatctgg
     8281 gtcattcata ataataatat ctgcatcatg tttaatacca tattcaagcg gtatttttca
     8341 tgcaggatca aattctggat ttggatttac aacattttta aatgtttcat cttcaaatgc
     8401 atgctcttca acctcaataa cgttatatcc tgattcacgt aatatttttg gggtaaattt
     8461 agttcctgtt ccattaactg cgctaaaaat aatttttaaa tcttttttag cttcttgctc
     8521 ttttttgtac gtctctgttt aagagctatg ctggaaacag catagcaagt ttaaataagg
     8581 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttaa tcgcttcatg
     8641 caaagcttgg cgtaactaga tcttgagaca aatggcagta ttcatccaca attttaaaag
     8701 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     8761 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     8821 cagggacagc agagatccac tttggcgccg gctcgagggg gcccgggtgc aaagatggat
     8881 aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt
     8941 gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     9001 gttgggggga ggggtcggca attgatccgg tgcctagaga aggtggcgcg gggtaaactg
     9061 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     9121 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt
     9181 aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc
     9241 ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag
     9301 tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga
     9361 ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct
     9421 cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt
     9481 tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt
     9541 tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg
     9601 cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct
     9661 ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc
     9721 ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa
     9781 atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc
     9841 ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca
     9901 cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta
     9961 tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt
    10021 gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc
    10081 tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgacgta cggccaccat
    10141 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
    10201 caccctc
//
