LOCUS       sequence-436968-        9603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Stably express GFP-tagged OPTN for CID induced mitophagy.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9603)
  AUTHORS   Adriaenssens E, Nguyen TN, Sawa-Makarska J, Khuu G, Schuschnig M, 
            Shoebridge S, Watts EM, Csalyi KD, Padman BS, Lazarou M, Martens S
  TITLE     Control of mitophagy initiation and progression by the TBK1 adaptors
            NAP1 and SINTBAD
  JOURNAL   bioRxiv September 25, 202
REFERENCE   2  (bases 1 to 9603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            September 25, 202"
COMMENT     Sequence Label: pHAGE-FKBP-GFP-OPTN
FEATURES             Location/Qualifiers
     source          1..9603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2062..2179
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2231..2534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2535..2738
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2689..2709
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2735..2759
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2821..2830
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2827..2829,2830..3150)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             3157..3477
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /note="synthetic gene with alternative codons"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             3157..3477
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             join(3496..3498,3499..4209)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3538..3559)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3799..3818)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4146..4167
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4569..4580
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    5993..6581
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6046..6066)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6464..6475)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6656..6889
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7008..7026)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7094..7198
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7199..7267,7268..8059)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7417..7436)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8230..8818
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8719..8738
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8972..8989
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9229..9250
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9265..9270,9271..9288,9289..9295)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9303..9319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9308..9330
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9327..9343
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9327..9343
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9393..9412
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttaattcaca
     2041 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2101 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2161 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
     2221 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2281 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2341 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2401 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2461 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2521 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2581 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2641 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     2701 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     2761 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccggcgg ccgcggtacc
     2821 gccaccatgg gagtgcaggt ggaaaccatc tccccaggag acgggcgcac cttccccaag
     2881 cgcggccaga cctgcgtggt gcactacacc gggatgcttg aagatggaaa gaaatttgat
     2941 tcctcccggg acagaaacaa gccctttaag tttatgctag gcaagcagga ggtgatccga
     3001 ggctgggaag aaggggttgc ccagatgagt gtgggtcaga gagccaaact gactatatct
     3061 ccagattatg cctatggtgc cactgggcac ccaggcatca tcccaccaca tgccactctc
     3121 gtcttcgatg tggagcttct aaaactggaa actagaggcg tgcaggtcga gaccatcagc
     3181 cccggcgacg gccgcacctt tcccaagaga ggccagactt gcgtggtcca ctacaccggc
     3241 atgctggagg acggcaagaa gttcgacagc agccgcgacc gcaacaagcc cttcaagttc
     3301 atgctgggca aacaggaagt gatccgcggc tgggaggaag gcgtggctca gatgagcgtg
     3361 gggcagcggg ccaagctgac catcagcccc gactatgcct acggcgccac cggccacccc
     3421 ggcatcatcc ccccccacgc caccctggtg ttcgacgtgg agctgctgaa gctggagact
     3481 agtaccggtt ctagagtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     3541 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     3601 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     3661 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     3721 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     3781 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     3841 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     3901 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     3961 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     4021 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     4081 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc
     4141 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     4201 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgactcccat
     4261 caacctctca gctgcctcac tgaaaaggag gacagcccca gtgaaagcac aggaaatgga
     4321 cccccccacc tggcccaccc aaacctggac acgtttaccc cggaggagct gctgcagcag
     4381 atgaaagagc tcctgaccga gaaccaccag ctgaaagaag ccatgaagct aaataatcaa
     4441 gccatgaaag ggagatttga ggagctttcg gcctggacag agaaacagaa ggaagaacgc
     4501 cagttttttg agatacagag caaagaagca aaagagcgtc taatggcctt gagtcatgag
     4561 aatgagaaat tgaaggaaga gcttggaaaa ctaaaaggga aatcagaaag gtcatctgag
     4621 gaccccactg atgactccag gcttcccagg gccgaagcgg agcaggaaaa ggaccagctc
     4681 aggacccagg tggtgaggct acaagcagag aaggcagacc tgttgggcat cgtgtctgaa
     4741 ctgcagctca agctgaactc cagcggctcc tcagaagatt cctttgttga aattaggatg
     4801 gctgaaggag aagcagaagg gtcagtaaaa gaaatcaagc atagtcctgg gcccacgaga
     4861 acagtctcca ctggcacggc attgtctaaa tataggagca gatctgcaga tggggccaag
     4921 aattacttcg aacatgagga gttaactgtg agccagctcc tgctgtgcct aagggaaggg
     4981 aatcagaagg tggagagact tgaagttgca ctcaaggagg ccaaagaaag agtttcagat
     5041 tttgaaaaga aaacaagtaa tcgttctgag attgaaaccc agacagaggg gagcacagag
     5101 aaagagaatg atgaagagaa aggcccggag actgttggaa gcgaagtgga agcactgaac
     5161 ctccaggtga catctctgtt taaggagctt caagaggctc atacaaaact cagcgaagct
     5221 gagctaatga agaagagact tcaagaaaag tgtcaggccc ttgaaaggaa aaattctgca
     5281 attccatcag agttgaatga aaagcaagag cttgtttata ctaacaaaaa gttagagcta
     5341 caagtggaaa gcatgctatc agaaatcaaa atggaacagg ctaaaacaga ggatgaaaag
     5401 tccaaattaa ctgtgctaca gatgacacac aacaagcttc ttcaagaaca taataatgca
     5461 ttgaaaacaa ttgaggaact aacaagaaaa gagtcagaaa aagtggacag ggcagtgctg
     5521 aaggaactga gtgaaaaact ggaactggca gagaaggctc tggcttccaa acagctgcaa
     5581 atggatgaaa tgaagcaaac cattgccaag caggaagagg acctggaaac catgaccatc
     5641 ctcagggctc agatggaagt ttactgttct gattttcatg ctgaaagagc agcgagagag
     5701 aaaattcatg aggaaaagga gcaactggca ttgcagctgg cagttctgct gaaagagaat
     5761 gatgctttcg aagacggagg caggcagtcc ttgatggaga tgcagagtcg tcatggggcg
     5821 agaacaagtg actctgacca gcaggcttac cttgttcaaa gaggagctga ggacagggac
     5881 tggcggcaac agcggaatat tccgattcat tcctgcccca agtgtggaga ggttctgcct
     5941 gacatagaca cgttacagat tcacgtgatg gattgcatca tttaaggatc ctaatcaacc
     6001 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     6061 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     6121 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     6181 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     6241 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     6301 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     6361 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     6421 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     6481 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     6541 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgagatcc tttaagacca
     6601 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     6661 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     6721 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     6781 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     6841 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     6901 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     6961 cccgggttaa ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc tcgtgatacg
     7021 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     7081 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     7141 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     7201 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     7261 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     7321 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     7381 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     7441 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     7501 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     7561 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     7621 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     7681 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     7741 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     7801 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     7861 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     7921 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     7981 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     8041 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     8101 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     8161 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     8221 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     8281 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     8341 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     8401 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     8461 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     8521 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     8581 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     8641 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     8701 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     8761 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     8821 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     8881 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     8941 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     9001 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagcaagct
     9061 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     9121 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctccccgtg
     9181 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     9241 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     9301 aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta cgaatttcac
     9361 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     9421 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
     9481 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
     9541 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
     9601 agt
//
