LOCUS       sequence-436973-       10422 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  vector for encoding an engineered glycosylase-based cytosine (C) 
            base editor (gCBEv3) driven by EF1a promoter, guide RNAs compatible 
            with SpG driven by hU6, mCherry driven by CBh promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10422)
  AUTHORS   Tong H, Wang H, Wang X, Liu N, Li G, Wu D, Li Y, Jin M, Li H, Wei Y,
            Li T, Yuan Y, Shi L, Yao X, Zhou Y, Yang H
  TITLE     Development of deaminase-free T-to-S base editor and C-to-G base 
            editor by engineered human uracil DNA glycosylase.
  JOURNAL   Nat Commun. 2024 Jun 8;15(1):4897. doi: 10.1038/s41467-024-49343-5.
  PUBMED    38851742
REFERENCE   2  (bases 1 to 10422)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-06-8"; volume: "15(1):4897. doi"; pages: " 
            10.1038/s41467-024-49343-5"
COMMENT     Sequence Label: gCBEv3
FEATURES             Location/Qualifiers
     source          1..10422
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        144..392
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     144..164
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     315..334
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        411..486
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        573..1751
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          804..1742
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1699..1719
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1768..1787
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1768..1786
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1836..1856
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1869..3584
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             6831..6851
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6863..6997
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6900..6919)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6954..6973
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     enhancer        7011..7296
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     intron          7586..7813
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      7832..7841
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7838..8548
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     DDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7983..8001)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    8571..8795
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(8803..9391)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8883..8902)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9562..10353,10354..10422))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEMGAS
                     LIKHW"
     primer_bind     10185..10204
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct gacgtctaag taggagggcc tatttcccat gattccttca tatttgcata
      181 tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat
      241 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
      301 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
      361 ctttatatat cttgtggaaa ggacgaaaca ccgggtcttc gagaagacct gttttagagc
      421 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
      481 cggtgctttt ttgttttaga gctagaaata gcaagttaaa ataaggctag tccgttttta
      541 gcgcgtgcgc caattctgca gacaaatggc tcggctccgg tgcccgtcag tgggcagagc
      601 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct
      661 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
      721 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
      781 acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct
      841 ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc
      901 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag
      961 ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa
     1021 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt
     1081 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag
     1141 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc
     1201 agcgcacatg ttcggcgagg cggggcctgc gagcgcggca accgagaatc ggacgggggt
     1261 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc
     1321 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc
     1381 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg
     1441 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca
     1501 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt
     1561 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga
     1621 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt
     1681 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc
     1741 aggtgtcgtg aggaattagc ttggtactaa tacgactcac tatagggaga gccgccacca
     1801 tgaaacggac agccgacgga agcgagttcg agtcaccaaa gaagaagcgg aaagtctctg
     1861 gcggctcaga caagaagtac agcatcggcc tggccatcgg caccaactct gtgggctggg
     1921 ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg
     1981 accggcacag catcaagaag aacctgatcg gagccctgct gttcgacagc ggcgaaacag
     2041 ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg aagaaccgga
     2101 tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac agcttcttcc
     2161 acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg caccccatct
     2221 tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc taccacctga
     2281 gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat ctggccctgg
     2341 cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac cccgacaaca
     2401 gcgacgtgga caagctgttc atccagctgg tgcagaccta caaccagctg ttcgaggaaa
     2461 accccatcaa cgccagcggc gtggacgcca aggccatcct gtctgccaga ctgagcaaga
     2521 gcagacggct ggaaaatctg atcgcccagc tgcccggcga gaagaagaat ggcctgttcg
     2581 gaaacctgat tgccctgagc ctgggcctga cccccaactt caagagcaac ttcgacctgg
     2641 ccgaggatgc caaactgcag ctgagcaagg acacctacga cgacgacctg gacaacctgc
     2701 tggcccagat cggcgaccag tacgccgacc tgtttctggc cgccaagaac ctgtccgacg
     2761 ccatcctgct gagcgacatc ctgagagtga acaccgagat caccaaggcc cccctgagcg
     2821 cctctatgat caagagatac gacgagcacc accaggacct gaccctgctg aaagctctcg
     2881 tgcggcagca gctgcctgag aagtacaaag agattttctt cgaccagagc aagaacggct
     2941 acgccggcta cattgacggc ggagccagcc aggaagagtt ctacaagttc atcaagccca
     3001 tcctggaaaa gatggacggc accgaggaac tgctcgtgaa gctgaacaga gaggacctgc
     3061 tgcggaagca gcggaccttc gacaacggca gcatccccca ccagatccac ctgggagagc
     3121 tgcacgccat tctgcggcgg caggaagatt tttacccatt cctgaaggac aaccgggaaa
     3181 agatcgagaa gatcctgacc ttccgcatcc cctactacgt gggccctctg gccaggggaa
     3241 acagcagatt cgcctggatg accagaaaga gcgaggaaac catcaccccc tggaacttcg
     3301 aggaagtggt ggacaagggc gcttccgccc agagcttcat cgagcggatg accaacttcg
     3361 ataagaacct gcccaacgag aaggtgctgc ccaagcacag cctgctgtac gagtacttca
     3421 ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga gggaatgaga aagcccgcct
     3481 tcctgagcgg cgagcagaaa aaggccatcg tggacctgct gttcaagacc aaccggaaag
     3541 tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat cgagtgcttc gactccgtgg
     3601 aaatctccgg cgtggaagat cggttcaacg cctccctggg cacataccac gatctgctga
     3661 aaattatcaa ggacaaggac ttcctggaca atgaggaaaa cgaggacatt ctggaagata
     3721 tcgtgctgac cctgacactg tttgaggaca gagagatgat cgaggaacgg ctgaaaacct
     3781 atgcccacct gttcgacgac aaagtgatga agcagctgaa gcggcggaga tacaccggct
     3841 ggggcaggct gagccggaag ctgatcaacg gcatccggga caagcagtcc ggcaagacaa
     3901 tcctggattt cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag ctgatccacg
     3961 acgacagcct gacctttaaa gaggacatcc agaaagccca ggtgtccggc cagggcgata
     4021 gcctgcacga gcacattgcc aatctggccg gcagccccgc cattaagaag ggcatcctgc
     4081 agacagtgaa ggtggtggac gagctcgtga aagtgatggg ccggcacaag cccgagaaca
     4141 tcgtgatcga aatggccaga gagaaccaga ccacccagaa gggacagaag aacagccgcg
     4201 agagaatgaa gcggatcgaa gagggcatca aagagctggg cagccagatc ctgaaagaac
     4261 accccgtgga aaacacccag ctgcagaacg agaagctgta cctgtactac ctgcagaatg
     4321 ggcgggatat gtacgtggac caggaactgg acatcaaccg gctgtccgac tacgatgtgg
     4381 accatatcgt gcctcagagc tttctgaagg acgactccat cgacaacaag gtgctgacca
     4441 gaagcgacaa gaaccggggc aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga
     4501 tgaagaacta ctggcggcag ctgctgaacg ccaagctgat tacccagaga aagttcgaca
     4561 atctgaccaa ggccgagaga ggcggcctga gcgaactgga taaggccggc ttcatcaaga
     4621 gacagctggt ggaaacccgg cagatcacaa agcacgtggc acagatcctg gactcccgga
     4681 tgaacactaa gtacgacgag aatgacaagc tgatccggga agtgaaagtg atcaccctga
     4741 agtccaagct ggtgtccgat ttccggaagg atttccagtt ttacaaagtg cgcgagatca
     4801 acaactacca ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc gccctgatca
     4861 aaaagtaccc taagctggaa agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc
     4921 ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc taccgccaag tacttcttct
     4981 acagcaacat catgaacttt ttcaagagtg gaggctcatc aggaggttca tcaggctcag
     5041 agacacccgg aacctctgaa agcgccacac cagagagctc tggtggatcc agtgggggtt
     5101 caaacgtgcc cgtgggcttt ggagagagct ggaagaagca cctcagcggg gagttcggga
     5161 aaccgtattt tatcaagcta atgggatttg ttgcagaaga aagaaagcat tacactgttt
     5221 atccaccccc acaccaagtg ttcacctgga cccagatgtg tgacataaaa gatgtgaagg
     5281 ttgtcatcct gggacaggat ccatatcatg gacctaatca agctcacggg ctctgcttta
     5341 gtgttcaaag gcctgttccg cctccgccca gtttggagaa catttatgcc gagttgtcta
     5401 cagacataga ggattttgtt catcctggcc atggagattt atctgggtgg gccaagcaag
     5461 gtgttctcct tctcgacgtg gtcctcacgg ttcgtgccca tcaagccaac tctcataagg
     5521 agcgaggctg ggagcagttc actgatgcag ttgtgtcctg gctaaatcag aactcgaatg
     5581 gccttgtttt cttgctctgg ggctcttatg ctcagaagaa gggcagtgcc attgatagga
     5641 agcggcacca tgtactacag acggctcatc cctccccttt gtcagtgtat agagggttct
     5701 ttggatgtag acacttttca aagaccaatg agctgctgca gaagtctggc aagaagccca
     5761 ttgactggaa ggagctgtcc gggggtagct ctggcggcag cagtggatca gagacacccg
     5821 gtacctccga gagcgcaacc ccagagtcct ctggtgggtc tagcggtggc agtgagacaa
     5881 acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag
     5941 tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca
     6001 gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact
     6061 gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg
     6121 tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga
     6181 tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg
     6241 gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc
     6301 tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac
     6361 tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga
     6421 agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc
     6481 tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta
     6541 atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg
     6601 ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt
     6661 actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca
     6721 ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg
     6781 gaggtgactc tggcggctca aaaagaaccg ccgacggcag cgaattcgag cccaagaaga
     6841 agaggaaagt ctaaacgcgt cgaacttgtt tattgcagct tataatggtt acaaataaag
     6901 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     6961 gtccaaactc atcaatgtat cttatcatgt ctggatcggc ggccggtacc cgttacataa
     7021 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     7081 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     7141 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     7201 ggtaaatggc ccgcctggca ttgtgcccag tacatgacct tatgggactt tcctacttgg
     7261 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
     7321 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
     7381 ttattttgtg cagcgatggg ggcggggggg gggggggggg ggggcccggg gggggggggg
     7441 gggggggggg gggggggggg gggggggggg ggcgaggcgg agaggtgcgg cggcagccaa
     7501 tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta
     7561 taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg
     7621 ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt
     7681 gagcgggcgg gacggccctt ctcctccggg ctgtaattag ctgagcaaga ggtaagggtt
     7741 taagggatgg ttggttggtg gggtattaat gtttaattac ctggagcacc tgcctgaaat
     7801 cacttttttt caggttggca gatccgctag cgccaccatg gtgagcaagg gcgaggagga
     7861 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
     7921 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     7981 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
     8041 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
     8101 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg atgacggcgg
     8161 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
     8221 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaaa ccatgggctg
     8281 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
     8341 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
     8401 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc
     8461 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
     8521 cggcggcatg gacgagctgt acaagtaaac cggtctcttg gcccggatcc ctgtgccttc
     8581 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     8641 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     8701 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaaaacaa
     8761 tagcaggcat gctggggatg cggtgggctc tatggtggaa gctttccata ggctccgccc
     8821 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8881 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8941 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     9001 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9061 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9121 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     9181 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     9241 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     9301 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     9361 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     9421 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     9481 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     9541 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccca tctcagcgat
     9601 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9661 ggagggctta ccatctggcc ccagtgctgc aatgataccg cggcttccac gctcaccggc
     9721 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     9781 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9841 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     9901 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9961 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    10021 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    10081 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10141 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10201 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    10261 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    10321 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    10381 aaaaaaggga ataagggcga cacggaaatg ttgaatactc at
//
