LOCUS       sequence-436981-       10352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  vector for encoding an engineered glycosylase-based thymine (T) base
            editor (gTBEv3) driven by EF1a promoter, guide RNAs compatible with 
            SpCas9 driven by hU6, mCherry driven by CBh promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10352)
  AUTHORS   Tong H, Wang H, Wang X, Liu N, Li G, Wu D, Li Y, Jin M, Li H, Wei Y,
            Li T, Yuan Y, Shi L, Yao X, Zhou Y, Yang H
  TITLE     Development of deaminase-free T-to-S base editor and C-to-G base 
            editor by engineered human uracil DNA glycosylase.
  JOURNAL   Nat Commun. 2024 Jun 8;15(1):4897. doi: 10.1038/s41467-024-49343-5.
  PUBMED    38851742
REFERENCE   2  (bases 1 to 10352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-06-8"; volume: "15(1):4897. doi"; pages: " 
            10.1038/s41467-024-49343-5"
COMMENT     Sequence Label: gTBEv3
FEATURES             Location/Qualifiers
     source          1..10352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        144..392
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     144..164
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     315..334
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        411..486
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        573..1751
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          804..1742
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1699..1719
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1768..1787
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1768..1786
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1836..1856
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2628..2651,2652..2654,2655..6728)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             2628..6728
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6771..6791
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6803..6937
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6840..6859)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6894..6913
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     enhancer        6951..7236
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        7238..7515
                     /label=chicken beta-actin promoter
     intron          7516..7743
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      7762..7771
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7768..8478
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     DDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7913..7931)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    8501..8725
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(8733..9321)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8813..8832)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9492..10283,10284..10352))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEMGAS
                     LIKHW"
     primer_bind     10115..10134
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct gacgtctaag taggagggcc tatttcccat gattccttca tatttgcata
      181 tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat
      241 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
      301 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
      361 ctttatatat cttgtggaaa ggacgaaaca ccgggtcttc gagaagacct gttttagagc
      421 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
      481 cggtgctttt ttgttttaga gctagaaata gcaagttaaa ataaggctag tccgttttta
      541 gcgcgtgcgc caattctgca gacaaatggc tcggctccgg tgcccgtcag tgggcagagc
      601 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct
      661 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
      721 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
      781 acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct
      841 ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc
      901 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag
      961 ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa
     1021 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt
     1081 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag
     1141 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc
     1201 agcgcacatg ttcggcgagg cggggcctgc gagcgcggca accgagaatc ggacgggggt
     1261 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc
     1321 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc
     1381 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg
     1441 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca
     1501 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt
     1561 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga
     1621 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt
     1681 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc
     1741 aggtgtcgtg aggaattagc ttggtactaa tacgactcac tatagggaga gccgccacca
     1801 tgaaacggac agccgacgga agcgagttcg agtcaccaaa gaagaagcgg aaagtcaacg
     1861 tgcccgtggg ctttggagag agctggaaga agcacctcag cggggagttc gggaaaccgt
     1921 attttatcaa gctaatggga tttgttgcag aagaaagaaa gcattacact gtttatccac
     1981 ccccacacca agtgttcacc tggacccaga tgtgtgacat aaaagatgtg aaggttgtca
     2041 tcctgggaca ggatccagcc catggaccta atcaagctca cgggctctgc tttagtgttc
     2101 aaaggcctgt tccgcctccg cccagtttgg agaacattta taaagagttg tctacagaca
     2161 tagaggattt tgttcatcct ggccatggag atttatctgg gtgggccaag caaggtgttc
     2221 tccttctcaa caccgtcctc acggttcgtg cccatcaagc caactctcat aaggagcgag
     2281 gctgggagca gttcactgat gcagttgtgt cctggctaaa tcagaactcg aatggccttg
     2341 ttttcttgct ctggggctct tatgctgcca agaagggcag tgccattgat aggaagcggc
     2401 accatgtact acagacggct catccctccc ctttgtcagt ggacagaggg ttctttggat
     2461 gtagacactt ttcaaagacc aatgagctgc tgcagaagtc tggcaagaag cccattgact
     2521 ggaaggagct gtccggagga tctagcggag gctcctctgg ctctgagaca cctggcacaa
     2581 gcgagagcgc aacacctgaa agcagcgggg gcagcagcgg ggggtcagac aagaagtaca
     2641 gcatcggcct ggccatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
     2701 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
     2761 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga
     2821 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
     2881 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
     2941 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
     3001 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca
     3061 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg
     3121 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca
     3181 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg
     3241 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga
     3301 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc
     3361 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc
     3421 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt
     3481 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc
     3541 tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg
     3601 acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga
     3661 agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg
     3721 gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca
     3781 ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg
     3841 acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc
     3901 aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct
     3961 tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga
     4021 ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg
     4081 cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga
     4141 aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca
     4201 aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa
     4261 aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag
     4321 aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc
     4381 ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact
     4441 tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt
     4501 ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca
     4561 aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc
     4621 tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg
     4681 acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag
     4741 aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca
     4801 atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg
     4861 agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag
     4921 agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag
     4981 agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc
     5041 tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc
     5101 aggaactgga catcaaccgg ctgtccgact acgatgtgga ccatatcgtg cctcagagct
     5161 ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca
     5221 agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc
     5281 tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag
     5341 gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc
     5401 agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga
     5461 atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt
     5521 tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg
     5581 acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa
     5641 gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg
     5701 agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt
     5761 tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa
     5821 acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag
     5881 tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca
     5941 gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact
     6001 gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg
     6061 tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga
     6121 tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg
     6181 gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc
     6241 tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac
     6301 tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga
     6361 agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc
     6421 tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta
     6481 atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg
     6541 ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt
     6601 actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca
     6661 ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg
     6721 gaggtgactc tggcggctca aaaagaaccg ccgacggcag cgaattcgag cccaagaaga
     6781 agaggaaagt ctaaacgcgt cgaacttgtt tattgcagct tataatggtt acaaataaag
     6841 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     6901 gtccaaactc atcaatgtat cttatcatgt ctggatcggc ggccggtacc cgttacataa
     6961 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     7021 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     7081 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     7141 ggtaaatggc ccgcctggca ttgtgcccag tacatgacct tatgggactt tcctacttgg
     7201 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
     7261 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
     7321 ttattttgtg cagcgatggg ggcggggggg gggggggggc gcgcgccagg cggggcgggg
     7381 cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc
     7441 gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga
     7501 agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc
     7561 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg
     7621 gacggccctt ctcctccggg ctgtaattag ctgagcaaga ggtaagggtt taagggatgg
     7681 ttggttggtg gggtattaat gtttaattac ctggagcacc tgcctgaaat cacttttttt
     7741 caggttggca gatccgctag cgccaccatg gtgagcaagg gcgaggagga taacatggcc
     7801 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     7861 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     7921 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     7981 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     8041 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg atgacggcgg cgtggtgacc
     8101 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     8161 accaacttcc cctccgacgg ccccgtaatg cagaagaaaa ccatgggctg ggaggcctcc
     8221 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     8281 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     8341 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     8401 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     8461 gacgagctgt acaagtaaac cggtctcttg gcccggatcc ctgtgccttc tagttgccag
     8521 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     8581 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     8641 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaaaacaa tagcaggcat
     8701 gctggggatg cggtgggctc tatggtggaa gctttccata ggctccgccc ccctgacgag
     8761 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     8821 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     8881 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     8941 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     9001 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     9061 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     9121 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     9181 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     9241 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     9301 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     9361 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     9421 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     9481 tggtctgaca gttaccaatg cttaatcagt gaggcaccca tctcagcgat ctgtctattt
     9541 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     9601 ccatctggcc ccagtgctgc aatgataccg cggcttccac gctcaccggc tccagattta
     9661 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     9721 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9781 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     9841 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9901 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9961 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    10021 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    10081 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    10141 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    10201 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    10261 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    10321 ataagggcga cacggaaatg ttgaatactc at
//
