LOCUS       sequence-436983-       11016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA 
            (BsaI site) and PG-dCas9-novo (for cloning new guides).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11016)
  AUTHORS   Hall AN, Hall BW, Kinney KJ, Olsen GG, Banta AB, Noguera DR, Donohue
            TJ, Peters JM
  TITLE     Tools for genetic engineering and gene expression control in 
            Novosphingobium aromaticivorans and Rhodobacter sphaeroides.
  JOURNAL   Appl Environ Microbiol. 2024 Sep 26:e0034824. doi: 
            10.1128/aem.00348-24.
  PUBMED    39324814
REFERENCE   2  (bases 1 to 11016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2024 Sep 26:e0034824. doi: 10.1128/aem.00348-24."
COMMENT     Sequence Label: pJMP8932
FEATURES             Location/Qualifiers
     source          1..11016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        171..248
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             249..1331
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(268..287)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     protein_bind    1608..1624
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    complement(2049..2096)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(2173..2178,2179..2195,2196..2201)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             2312..3106
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2366..2385)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2976..2995
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    complement(3158..3205)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(3256..3342)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(3445..3539)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(3567..3583)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3568..3584)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(3998..4017)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4117..4139
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4177..4195)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4263..4367
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4368..4436,4437..5228)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4586..4605)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5529..5917
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            6186..6294
                     /note="incP origin of transfer"
     mobile_element  6398..6563
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     CDS             complement(6718..6807)
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     CDS             complement(join(6817..8400,8401..8403,8404..10890,
                     10891..10893,10894..10920))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     protein_bind    10967..10983
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tctgcagacg taaaaaaagc ggcgtggtta gccgcttttt taattgccgg agcgcaataa
       61 aaaagccccc ggaaggtgat cttccggggg ctttctcatg cgttgcttgt attgaaaatg
      121 ggggccatgc ctacctatct gcctcccggg aagcggcatg catttacgtt gacaccatcg
      181 aatggtgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt caattcaggg
      241 tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc
      301 agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag
      361 tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg
      421 gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc
      481 aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga
      541 tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac
      601 gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag
      661 ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca
      721 gtattatttt ctcccatgaa gatggtacgc gactgggcgt ggagcatctg gtcgcattgg
      781 gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc
      841 tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag
      901 gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg
      961 ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta
     1021 ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag
     1081 atagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc
     1141 aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc
     1201 tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct
     1261 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     1321 gcgggcagtg agcgcaacgc aattaatgta agttagctca ctcattaggc accccacccg
     1381 ggtccttgag ccccattatc acctccaaag ccttctcgtc tggtcagttt cacctgtttt
     1441 acgtaaaaac ccgcttcggc gggtttttac ttttggggaa acacagaaaa aagcccgcac
     1501 ctgacagtgc gggctttttt tttcgaccaa aggtgcgact actcttgcct actacctatc
     1561 gactgagctg aaagaattcg gaaaattttt tttcaaaagt acttgaattg tgagcggata
     1621 acaattataa ttctagttag ttgagaccaa ctttggtctc caccatagcg gtcggtctct
     1681 gtttaagagc tatgctggaa acagcatagc aagtttaaat aaggctagtc cgttatcaac
     1741 ttgaaaaagt ggcaccgagt cggtgctttt tttgaattca tgtggctgac cgttctgttg
     1801 tctctcgctc ttccgagtag acgaacaata aggcctccct aacggggggc cttttttatt
     1861 gataacaaaa gtcagtgctt ccgctatttc caaaataccg ggctaatacg gtttaaacga
     1921 cctcctggat ttgctcagac agccttttcg tcattcgttt cagccaaaaa acttaagacc
     1981 gccggtcttg tccactacct tgcagtaatg cggtggacag gatcggcggt tttcttttct
     2041 cttctcaaga agttcctata ctttctagag aataggaact tcggaatagg aacttcctcc
     2101 tgaacggcca taagaacgaa ggctgtctgt tgaactctcg agccgtggaa acggatgaag
     2161 gcacgaaccc agttgacata agcctgttcg gttcgtaaac tgtaatgcaa gtagcgtatg
     2221 cgctcaaaac tggatggctt tcttgccgcc aaggatctga tggcgcaggg gatcaagatc
     2281 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     2341 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     2401 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     2461 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     2521 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     2581 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     2641 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     2701 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     2761 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     2821 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     2881 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     2941 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     3001 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     3061 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     3121 ttcgaaatga ctcgagcatc agcttcaaaa gcgctctgaa gttcctatac tttctagaga
     3181 ataggaactt cggaataggt acttcaagat ccccaattcg agctcatcgt ccgggccgca
     3241 agctcctagc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     3301 acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcctca agctagagag
     3361 tcattacccc aggcgtttaa gggcaccaat aactgcctta aaaaaattac gccccgccct
     3421 gccactcatc gcagtctagc ttggattctc accaataaaa aacgcccggc ggcaaccgag
     3481 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
     3541 aagctcagct aattaagcta gcttatcgat accgtcgacc tcgaacccca cgcccctctt
     3601 taatacgacg ggcaatttgc acttcagaaa atgaagagtt tgctttagcc ataacaaaag
     3661 tccagtatgc tttttcacag cataactgga ctgatttcag tttacaacta ttctgtctag
     3721 tttaagactt tattgtcata gtttagatct attttgttca gtttaagact ttattgtccg
     3781 cccacacctg cttacgcagg gcatccattt attactcaac cgtaaccgat tttgccaggt
     3841 tacgcggctg gtcctctagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     3901 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc
     3961 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
     4021 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     4081 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     4141 tttcaccgtc gtcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     4201 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     4261 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4321 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     4381 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     4441 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     4501 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     4561 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     4621 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     4681 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     4741 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     4801 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     4861 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     4921 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     4981 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     5041 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtcccgc ggtatcattg
     5101 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     5161 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     5221 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     5281 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     5341 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggcagttatt
     5401 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5461 attgatttaa aacttcattt ttaatttttg cggccgcaag atccggccac gatgcgtccg
     5521 gcgtagagga tctgaagatc agcagttcaa cctgttgata gtacgtacta agctctcatg
     5581 tttcacgtac taagctctca tgtttaacgt actaagctct catgtttaac gaactaaacc
     5641 ctcatggcta acgtactaag ctctcatggc taacgtacta agctctcatg tttcacgtac
     5701 taagctctca tgtttgaaca ataaaattaa tataaatcag caacttaaat agcctctaag
     5761 gttttaagtt ttataagaaa aaaaagaata tataaggctt ttaaagcttt taaggtttaa
     5821 cggttgtgga caacaagcca gggatgtaac gcactgagaa gcccttagag cctctcaaag
     5881 caattttgag tgacacagga acacttaacg gctgacatgg gaattagcca tggcatcaca
     5941 gtatcgtgat gacagaggca gggagtgcgg cctttttacg gttcctggcc ttttgctggc
     6001 cttttgctca catgttcttt cctgcgttat caggggattc cttaaggtat actttccgct
     6061 gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc ctttttcgca
     6121 cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat ccaacggcgt
     6181 cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt cttcactgtc
     6241 ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg gccgataagc
     6301 tctgataccg ctcgccgcag ccgaacgacc gagcgcagcg ggtcagtgag cgaggaagcg
     6361 gaagagccca aacataacag gaagaaaaat gccccgctgt gggcggacaa aatagttggg
     6421 aactgggagg ggtggaaatg gagtttttaa ggattattta gggaagagtg acaaaataga
     6481 tgggaactgg gtgtagcgtc gtaagctaat acgaaaatta aaaatgacaa aatagtttgg
     6541 aactagattt cacttatctg gttcttgagg cggttaaaag agccgtactc ttctccgatg
     6601 tcgactaggc catgatgctc attctgtggg accaaaacga aaaaacaccc tttcgggtgt
     6661 cttttctgga atttggtacc gaggctgcaa gtcccattaa ggaggcgcgc cttattacag
     6721 atcttcctca gagatgagct tctgttccag atcttcttct gaaatcaact tttgttccag
     6781 atcttcttca gagatgagtt tctgctccgc ggccgcgtcg ccgcccagct gcgacaggtc
     6841 gatgcgggtt tcgtacaggc cggtgatcga ctgatggatc agggtggcgt ccaggacctc
     6901 tttggtcgag gtgtagcgct tgcggtcgat ggtggtgtcg aagtacttga aggcggccgg
     6961 ggcgcccagg ttggtcaggg tgaacaggtg gatgatgttc tcggcttgct cgcggatcgg
     7021 cttgtcgcgg tgcttgttat aggccgacag gaccttgtcc aggttggcgt cggccaggat
     7081 gacgcgcttc gagaactccg agatctgctc gatgatctcg tccaggtagt gcttgtgctg
     7141 ctcgacgaac agctgcttct gctcgttgtc ctccggcgag cccttcagct tctcgtagtg
     7201 cgaggccagg tacaggaagt tgacgtactt cgacggcagg gccagctcgt tgcccttctg
     7261 cagctcgccg gcagaagcca gcattcgctt ccggccgttt tctaactcga acagcgagta
     7321 cttcggcagc ttgatgatca ggtccttctt gacctctttg tagcccttgg cctccaggaa
     7381 gtcgatcggg ttcttctcga acgacgagcg ctccatgatg gtaatgccca gtaactcctt
     7441 gaccgacttc agtttcttcg acttgccctt ctcgaccttg gcgacgacca ggaccgagta
     7501 ggcgacggtc ggcgagtcga agccgccgta cttcttcggg tcccagtcct tcttgcgtgc
     7561 gatcagcttg tccgagttgc gctttggcag gatcgactcc ttcgagaagc cgccggtctg
     7621 cacctcggtt ttcttgacaa tgttgacttg cggcatgctt aagactttgc ggacggtggc
     7681 gaagtcgcgg cccttgtccc agacgatctc tccggtttcc ccgtttgtct cgatcagcgg
     7741 gcgcttgcgg atctcgccgt tggccagggt gatctcggtc ttgaagaagt tcatgatgtt
     7801 cgagtagaag aagtacttgg cggtggcctt gccgatctct tgctccgact tggcgatcat
     7861 cttgcggacg tcgtagacct tgtagtcgcc gtagacgaac tccgactcca gttttgggta
     7921 cttcttgatc agggcggtgc cgacgacggc gttcaggtag gcgtcgtggg cgtggtggta
     7981 gttgttgatc tcgcggacct tgtagaactg aaagtccttg cggaagtccg agacgagctt
     8041 cgacttcagg gtgatgacct tgacctcgcg aatcagctta tcgttctcgt cgtacttggt
     8101 gttcatgcgc gagtccagga tctgcgcgac gtgcttggtg atctgccggg tttcgaccag
     8161 ctgccgcttg atgaagccgg ccttgtccag ctccgacagg ccgccgcgct cggccttggt
     8221 caggttgtcg aacttgcgct gcgtgatcag cttggcgttc agcagctgcc gccagtagtt
     8281 cttcatcttc ttaacgacct cttccgacgg gacgttgtcc gacttgccgc ggttcttgtc
     8341 cgagcgggtc aggaccttgt tgtcgatgct atcgtccttc aggaaggact gcgggacgat
     8401 ggcgtcgacg tcgtagtccg acaggcggtt gatgtccagc tcttggtcga cgtacatgtc
     8461 gcggccgttc tgcaggtagt acaggtacag cttctcgttc tgcagctgcg tgttctcgac
     8521 cgggtgctcc ttcaggatct gcgagcccag ctccttgatg ccctcctcga tgcgcttcat
     8581 gcgctcgcgc gaatttttct gtcccttctg cgtggtctga ttctcgcggg ccatctcgat
     8641 gacgatgttc tccggcttgt ggcgtcccat taccttaacc agctcgtcga cgaccttgac
     8701 ggtctgcagg atgcccttct tgatggccgg cgagccggcc aggttggcga tgtgctcgtg
     8761 cagcgagtcg ccttggcccg agacttgggc cttctgaatg tcctccttga aggtcagcga
     8821 gtcgtcgtgg atcagctgca tgaagttgcg gttggcgaag ccgtccgact tcaggaagtc
     8881 caggatggtc ttgcccgact gcttgtcgcg gatgccgttg atcagcttgc gcgacaggcg
     8941 gccccagccg gtgtagcggc ggcgctttaa ctgcttcatg accttgtcgt cgaacaggtg
     9001 ggcgtaggtc ttgaggcgct cctcgatcat ctcgcggtcc tcgaacaggg tcagggtcag
     9061 gacgatgtcc tccaggatgt cctcgttctc ctcgttgtcc aggaagtcct tgtccttgat
     9121 gatcttcagc aggtcgtggt aggtgcccag cgaggcgttg aagcggtcct cgacgccgct
     9181 gatctcgacc gagtcgaagc actcgatctt cttgaagtag tcctctttca gctgcttgac
     9241 ggtgaccttg cggttggtct tgaacagcag gtcgacgatg gccttcttct gctcgcccga
     9301 caggaaggcc ggcttgcgca tgccctcggt gacgtacttg accttggtca gctcgttgta
     9361 gacggtgaag tactcgtaca gcaggctgtg cttaggcagg accttctcgt tcggcaggtt
     9421 cttgtcgaag ttggtcatgc gctcgatgaa cgactgcgcc gaggcgccct tgtcgacgac
     9481 ctcttcgaag ttccacgggg tgatggtttc ctccgacttg cgggtcatcc aggcgaagcg
     9541 cgagttcccg cgggccagtg gaccgacata gtacggtatg cgaaaggtca ggatcttctc
     9601 gatcttctct cgattgtcct tcaggaacgg gtagaagtcc tcttggcgtc tcaggatggc
     9661 gtgcagctcg cccaggtgga tctgatgcgg gatcgagccg ttgtcgaagg tgcgctgctt
     9721 gcgcagcagg tcctcgcggt tcagcttcac cagcagctcc tcggtgccgt ccatcttctc
     9781 caggatcggc ttgatgaact tgtagaactc ctcttgcgag gcgccgccgt cgatgtagcc
     9841 ggcgtagccg ttcttcgact gatcaaagaa gatctccttg tacttctccg gcagctgctg
     9901 ccggaccagg gccttcagca gggtcaggtc ttggtggtgc tcgtcgtagc gcttgatcat
     9961 cgaggccgac agcggggcct ttgtgatctc tgtgttgacg cgcaggatgt ctgacaggag
    10021 gattgcgtcc gacaggttct tggcggccag gaacaggtcg gcgtactgat cgccgatctg
    10081 cgccagcagg ttgtccaggt cgtcgtcgta ggtgtccttc gacagctgca gcttggcgtc
    10141 ctcggccagg tcgaagttcg acttgaagtt cggggtcagg cccagcgaca gggctatcaa
    10201 gttgccgaac aggccgttct tcttctcgcc cggcagttga gcaataagat tctccaggcg
    10261 gcgcgacttc gacaggcggg ccgacaggat ggccttagca tcaacgcccg aggcgttgat
    10321 cgggttctcc tcgaacagct gattgtaggt ctgcaccagc tgaatgaaca gcttgtcgac
    10381 gtccgagttg tccgggttca ggtcgccctc gatcaggaag tggccgcgga acttgatcat
    10441 gtgggccagg gccaggtaga tcaggcgcag gtcggccttg tcggtcgagt cgaccagctt
    10501 cttgcgcagg tggtagatgg tcggatactt ctcgtggtag gcgacctcgt cgacgatgtt
    10561 gccgaagatc gggtggcgct cgtgcttctt gtcctcctcg accaggaacg actcctccag
    10621 gcggtggaag aacgagtcgt cgaccttggc catctcgtta gaaaagatct cttgcaggta
    10681 gcagatgcgg ttcttgcgcc gcgtgtagcg gcgccgggcg gtcctcttca ggcgtgtggc
    10741 ctcggccgtc tccccagaat cgaatagcag cgccccgatc aaattcttct tgatcgagtg
    10801 gcggtcggtg ttgcccagga ccttgaactt cttcgacggg accttgtact cgtcggtgat
    10861 gacggcccag ccgaccgagt tggtgccgat ggccaggccg atcgagtact tcttgtccat
    10921 actagtatag tcctccttct cgctcttaat taaactagga ttataattgt tatccgctca
    10981 caattcaagt gtttttcgaa aaaaaatttc tactag
//
