LOCUS       sequence-43699-e         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbB4a-GFP
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      36..107
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      123..150
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 taaggatcca aactcgagta aggatctcca ggcatcaaat aaaacgaaag gctcagtcga
       61 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
      121 ctggctcacc ttcgggtggg cctttctgcg tttataccta ggctacagcc gatagtctgg
      181 aacagcgcac ttacgggttg ctgcgcaacc caagtgctac cggcgcggca gcgtgacccg
      241 tgtcggcggc tccaacggct cgccatcgtc cagaaaacac ggctcatcgg gcatcggcag
      301 gcgctgctgc ccgcgccgtt cccattcctc cgtttcggtc aaggctggca ggtctggttc
      361 catgcccgga atgccgggct ggctgggcgg ctcctcgccg gggccggtcg gtagttgctg
      421 ctcgcccgga tacagggtcg ggatgcggcg caggtcgcca tgccccaaca gcgattcgtc
      481 ctggtcgtcg tgatcaacca ccacggcggc actgaacacc gacaggcgca actggtcgcg
      541 gggctggccc cacgccacgc ggtcattgac cacgtaggcc aacacggtgc cggggccgtt
      601 gagcttcacg acggagatcc agcgctcggc caccaagtcc ttgactgcgt attggaccgt
      661 ccgcaaagaa cgtccgatga gcttggaaag tgtcttctgg ctgaccacca cggcgttctg
      721 gtggcccatc tgcgccacga ggtgatgcag cagc
//
