LOCUS       sequence-437009-        9259 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient expression in mammalian cells of SARS-CoV-2 (A1) spike 
            protein tagged on the C-terminus with HiBiT, the high affinity small
            fragment of split nanoluciferase assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9259)
  AUTHORS   Broderick K, Moutaoufik MT, Saccon T, Malty R, Amin S, Phanse S, 
            Joseph TP, Zilocchi M, Hosseinnia A, Istace Z, Hajikarimlou M, Abrar
            S, Fisher J, Brassard R, Perera R, Kumar A, Aoki H, Rahmatbakhsh M, 
            Jessulat M, Kobasa D, Dehne F, Prasad B, Gagarinova A, Joanne 
            Lemieux M, Cochrane A, Houry WA, Aly KA, Golshani A, Babu M
  TITLE     Human protein interaction networks of ancestral and variant 
            SARS-CoV-2 in organ-specific cells and bodily fluids.
  JOURNAL   Nat Commun. 2025 Jul 1;16(1):5784. doi: 10.1038/s41467-025-60949-1.
  PUBMED    40593736
REFERENCE   2  (bases 1 to 9259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-07-1"; volume: "16(1):5784. doi"; pages: " 
            10.1038/s41467-025-60949-1"
COMMENT     Sequence Label: pcDNA3.1-SARS2-Spike-HiBiT
FEATURES             Location/Qualifiers
     source          1..9259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        79..458
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        459..662
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     613..633
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     707..726
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        707..725
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4694..4711)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4700..4924
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4970..5398
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5057..5076)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5267..5288
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5407..5427)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5412..5741
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5592..5727
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5654..5673
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5808..6602
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5862..5881)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6472..6491
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6776..6897
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6813..6832)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6867..6886
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6946..6962)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6946..6962)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6959..6981)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6970..6986
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6994..7000,7001..7018,7019..7024))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7039..7060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7177..7194)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7348..7936)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7428..7447)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8107..8898,8899..8967))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8730..8749
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8968..9072)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9147..9166)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
       61 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
      121 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      181 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      241 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      301 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      361 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      421 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      481 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      541 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      601 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      661 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
      721 atagggagac ccaagctggc tagcatgttt gtcttcctgg tcctgctgcc tctggtctcg
      781 tctcagtgcg tgaacctgac tactagaacc cagctgcctc ctgcctatac taactccttc
      841 acccgcggcg tgtactaccc agacaaggtg ttccgcagct ccgtgctgca ctccacccag
      901 gatctgttcc tgcccttctt cagcaacgtg acctggttcc acgccatcca cgtgagcggc
      961 accaatggca ccaagcggtt cgacaatccc gtgctgccat tcaacgatgg cgtgtacttc
     1021 gcctccaccg agaagagcaa catcatccgc ggctggatct tcggcaccac cctggactcc
     1081 aagacccaga gcctgctgat cgtgaacaat gccaccaacg tggtcatcaa ggtgtgcgag
     1141 ttccagttct gcaatgatcc attcctgggc gtgtactacc acaagaacaa taagtcctgg
     1201 atggagagcg agttccgcgt gtacagctcc gccaacaatt gcaccttcga gtacgtgtcc
     1261 cagcccttcc tgatggacct ggagggcaag cagggcaatt tcaagaacct gcgcgagttc
     1321 gtgttcaaga atatcgatgg ctacttcaag atctactcca agcacacccc catcaacctg
     1381 gtgcgcgacc tgccacaggg cttcagcgcc ctggagccac tggtggatct gccaatcggc
     1441 atcaacatca ccaggttcca gaccctgctg gccctgcacc gcagctacct gaccccaggc
     1501 gacagctcca gcggatggac cgctggagct gctgcctact acgtgggcta cctgcagccc
     1561 cgcaccttcc tgctgaagta caacgagaat ggcaccatca ccgacgccgt ggattgcgcc
     1621 ctggatccac tgtccgagac aaagtgcacc ctgaagagct tcaccgtgga gaagggcatc
     1681 taccagacct ccaatttccg cgtgcagcca accgagagca tcgtgcgctt ccccaatatc
     1741 accaacctgt gcccattcgg cgaggtgttc aacgctacca ggttcgccag cgtgtacgct
     1801 tggaatcgca agcgcatctc caactgcgtg gccgactaca gcgtgctgta caactccgcc
     1861 agcttctcca ccttcaagtg ctacggcgtg tcccccacca agctgaatga tctgtgcttc
     1921 accaacgtgt acgccgatag cttcgtgatc aggggcgacg aggtgcgcca gatcgctcca
     1981 ggacagaccg gcaagatcgc tgactacaat tacaagctgc ccgacgattt caccggctgc
     2041 gtgatcgcct ggaactccaa caatctggat agcaaagtgg gcggcaacta caattacctg
     2101 taccgcctgt tccgcaagtc caatctgaag ccattcgagc gcgacatctc caccgagatc
     2161 taccaggctg gaagcacccc atgcaatgga gtggagggct tcaactgcta cttccccctg
     2221 cagagctacg gcttccagcc aaccaacgga gtgggatacc agccatacag ggtggtggtg
     2281 ctgtccttcg agctgctgca cgctccagct accgtgtgcg gaccaaagaa gagcaccaat
     2341 ctggtgaaga acaagtgcgt gaacttcaat ttcaacggcc tgaccggaac cggcgtgctg
     2401 accgagtcca acaagaagtt cctgccattc cagcagttcg gaagggacat cgctgatacc
     2461 accgacgccg tgcgcgaccc acagaccctg gagatcctgg atatcacccc atgctccttc
     2521 ggcggcgtga gcgtgatcac cccaggaacc aataccagca accaggtggc cgtgctgtac
     2581 caggacgtga attgcaccga ggtgccagtg gctatccacg ctgatcagct gaccccaacc
     2641 tggcgcgtgt acagcaccgg atccaacgtg ttccagaccc gcgccggatg cctgatcgga
     2701 gctgagcacg tgaacaattc ctacgagtgc gacatcccaa tcggagctgg aatctgcgcc
     2761 agctaccaga cccagaccaa ctccccaagg agggctcgca gcgtggccag ccagtccatc
     2821 atcgcctaca ccatgtccct gggcgccgag aatagcgtgg cctacagcaa caattccatc
     2881 gccatcccaa ccaacttcac catctccgtg accaccgaga tcctgcccgt gtccatgacc
     2941 aagaccagcg tggactgcac catgtacatc tgcggcgatt ccaccgagtg cagcaacctg
     3001 ctgctgcagt acggcagctt ctgcacccag ctgaatcgcg ccctgaccgg aatcgctgtg
     3061 gagcaggata agaacaccca ggaggtgttc gcccaggtga agcagatcta caagaccccc
     3121 ccaatcaagg acttcggcgg cttcaatttc agccagatcc tgcccgatcc aagcaagccc
     3181 tccaagcgca gcttcatcga ggacctgctg ttcaacaagg tgaccctggc cgatgccggc
     3241 ttcatcaagc agtacggcga ttgcctgggc gacatcgctg cccgcgacct gatctgcgcc
     3301 cagaagttca atggcctgac cgtgctgcca ccactgctga ccgatgagat gatcgctcag
     3361 tacacctccg ccctgctggc cggaaccatc accagcggat ggaccttcgg cgctggagcc
     3421 gccctgcaga tccccttcgc catgcagatg gcctaccgct tcaacggcat cggcgtgacc
     3481 cagaatgtgc tgtacgagaa ccagaagctg atcgccaatc agttcaactc cgccatcggc
     3541 aagatccagg actccctgtc cagcaccgcc agcgccctgg gcaagctgca ggatgtggtg
     3601 aatcagaacg cccaggccct gaataccctg gtgaagcagc tgtccagcaa cttcggcgcc
     3661 atctccagcg tgctgaatga tatcctgagc cgcctggaca aggtggaggc tgaggtgcag
     3721 atcgataggc tgatcaccgg ccgcctgcag tccctgcaga cctacgtgac ccagcagctg
     3781 atcagggctg ctgagatcag ggccagcgcc aatctggctg ctaccaagat gtccgagtgc
     3841 gtgctgggac agagcaagag ggtggacttc tgcggcaagg gctaccacct gatgtccttc
     3901 ccacagagcg ccccacacgg agtggtgttc ctgcacgtga cctacgtgcc agcccaggag
     3961 aagaacttca ccaccgctcc agctatctgc cacgatggca aggctcactt cccacgcgag
     4021 ggcgtgttcg tgtccaacgg cacccactgg ttcgtgaccc agcgcaattt ctacgagccc
     4081 cagatcatca ccaccgacaa taccttcgtg agcggcaact gcgacgtggt catcggaatc
     4141 gtgaacaata ccgtgtacga tcccctgcag ccagagctgg actccttcaa ggaggagctg
     4201 gataagtact tcaagaatca caccagcccc gacgtggatc tgggcgacat ctccggcatc
     4261 aatgccagcg tggtgaacat ccagaaggag atcgaccgcc tgaacgaggt ggccaagaat
     4321 ctgaacgagt ccctgatcga tctgcaggag ctgggcaagt acgagcagta catcaagtgg
     4381 ccatggtaca tctggctggg cttcatcgcc ggcctgatcg ccatcgtgat ggtgaccatc
     4441 atgctgtgct gcatgacctc ctgctgcagc tgcctgaagg gctgctgctc ctgcggcagc
     4501 tgctgcaagt tcgatgagga cgatagcgag cccgtgctga agggcgtcaa actgcactat
     4561 acaggctcca ccgagacatc ccaggtcgct cccgctggta gtgcaggtgg tagtgcaggt
     4621 gtgagtggct ggcgactgtt caagaagatc agctgactcg agtctagagg gcccgtttaa
     4681 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     4741 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     4801 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     4861 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     4921 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     4981 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     5041 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     5101 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     5161 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     5221 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     5281 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     5341 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     5401 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     5461 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     5521 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     5581 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     5641 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     5701 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     5761 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     5821 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     5881 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     5941 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     6001 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     6061 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     6121 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     6181 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     6241 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     6301 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     6361 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     6421 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     6481 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     6541 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     6601 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     6661 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     6721 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     6781 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6841 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     6901 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     6961 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7021 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7081 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     7141 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7201 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7261 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7321 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7381 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7441 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7501 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7561 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7621 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7681 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7741 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7801 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7861 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7921 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7981 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8041 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8101 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8161 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     8221 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     8281 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     8341 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8401 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8461 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8521 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8581 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8641 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8701 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8761 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8821 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8881 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8941 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     9001 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     9061 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
     9121 cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     9181 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct
     9241 acaacaaggc aaggcttga
//
