LOCUS       sequence-43703-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbB4k-GFP
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      43..114
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      130..157
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(224..886)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVLAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
ORIGIN
        1 ctacaaataa ggatccaaac tcgagtaagg atctccaggc atcaaataaa acgaaaggct
       61 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      121 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atacctaggc tacagccgat
      181 agtctggaac agcgcactta cgggttgctg cgcaacccaa gtgctaccgg cgcggcagcg
      241 tgacccgtgt cggcggctcc aacggctcgc catcgtccag aaaacacggc tcatcgggca
      301 tcggcaggcg ctgctgcccg cgccgttccc attcctccgt ttcggtcaag gctggcaggt
      361 ctggttccat gcccggaatg ccgggctggc tgggcggctc ctcgccgggg ccggtcggta
      421 gttgctgctc gcccggatac agggtcggga tgcggcgcag gtcgccatgc cccaacagcg
      481 attcgtcctg gtcgtcgtga tcaaccacca cggcggcact gaacaccgac aggcgcaact
      541 ggtcgcgggg ctggccccac gccacgcggt cattgaccac gtaggccaac acggtgccgg
      601 ggccgttgag cttcacgacg gagatccagc gctcggccac caagtccttg actgcgtatt
      661 ggaccgtccg caaagaacgt ccgatgagct tggaaagtgt cttctggctg accaccacgg
      721 cgttctggtg gcccatctgc gccacgaggt gatgcagcag cattgccgcc gtgggtttcc
      781 tcgcaataag cccggcccac gcctcatgcg ctttgcgttc cgtttgcacc cagtgaccgg
      841 gcttgttctt ggcttgaatg ccgatttctc tggactgcgt ggccatgctt a
//
