LOCUS       sequence-437036-        9938 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression vector of eGFP tagged TONSL siRNA resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9938)
  AUTHORS   O'Donnell L, Panier S, Wildenhain J, Tkach JM, Al-Hakim A, Landry 
            MC, Escribano-Diaz C, Szilard RK, Young JT, Munro M, Canny MD, Kolas
            NK, Zhang W, Harding SM, Ylanko J, Mendez M, Mullin M, Sun T, 
            Habermann B, Datti A, Bristow RG, Gingras AC, Tyers MD, Brown GW, 
            Durocher D
  TITLE     The MMS22L-TONSL complex mediates recovery from replication stress 
            and homologous recombination.
  JOURNAL   Mol Cell. 2010 Nov 24;40(4):619-31. doi: 
            10.1016/j.molcel.2010.10.024. Epub 2010 Nov 4.
  PUBMED    21055983
REFERENCE   2  (bases 1 to 9938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2010.10.024"; journalName: "Mol Cell"; date: 
            "2010-11-24- 24"; volume: "40"; issue: "4"; pages: "619-31"
COMMENT     Sequence Label: pDNA5-FRT/TO-eGFP-TONSL
FEATURES             Location/Qualifiers
     source          1..9938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(990..992,993..995,996..1706)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(990..992,993..995,996..1706)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1035..1056)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1296..1315)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1643..1664
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5890..5907)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5896..6120
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6253..6272)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6404..6451
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6459..7481
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7611..7732
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7648..7667)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7702..7721
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7781..7797)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7781..7797)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7794..7816)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7805..7821
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7829..7835,7836..7853,7854..7859))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7874..7895
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8012..8029)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8183..8771)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8263..8282)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8942..9733,9734..9802))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9565..9584
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9803..9907)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtacca tggtgagcaa gggcgaggag ctgttcaccg
     1021 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     1081 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     1141 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     1201 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     1261 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     1321 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     1381 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     1441 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     1501 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     1561 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     1621 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     1681 ctctcggcat ggacgagctg tacaagggcg cgccaatgag cctggagcgc gagcttcgcc
     1741 agctgagcaa ggcgaaagcc aaggcgcaga gggccgggca gcggcgcgaa gaggccgcgc
     1801 tgtgccacca gctgggggag ctcctggccg gccatggccg ctacgccgag gctctggagc
     1861 agcactggca ggagctgcag cttcgggagc gcgctgacga ccctctgggc tgtgccgtgg
     1921 cccaccgcaa gatcggagag cgcctggccg agatggagga ctacccggct gccttgcagc
     1981 accagcacca gtacctggag ctggcacatt ccctgcgcaa ccacacggag ctgcagaggg
     2041 cctgggccac catcggccgc acccacctgg acatctatga ccactgccag tcgagggatg
     2101 ctttgctgca ggcacaggct gcctttgaga agagcttggc tattgtggat gaggagctgg
     2161 aggggacact ggcccaggga gagctgaatg agatgaggac ccgcctctat ctcaacctgg
     2221 gcctcacctt tgagagcctg cagcagacag ccctgtgcaa cgattacttc aggaagagca
     2281 tcttccttgc ggagcagaac cacctttacg aggacctatt ccgcgcccgc tacaacctgg
     2341 gcaccatcca ctggcgcgcg ggccagcact cccaggctat gcgctgcttg gagggtgccc
     2401 gggagtgtgc gcacaccatg aggaagcggt tcatggagag cgagtgctgc gtggttattg
     2461 cacaggtcct ccaagacctg ggagactttt tggctgccaa gcgagccctg aagaaggcct
     2521 acaggctggg ctcccagaag cctgtgcaga gggcagccat ctgtcagaac ctccagcatg
     2581 tgctggcagt ggtccggctg cagcaacagc tggaagaggc tgagggcaga gaccctcagg
     2641 gtgccatggt catctgtgag cagctagggg acctcttctc caaggcagga gactttccca
     2701 gggcagctga ggcttaccag aagcagctgc gttttgctga gctgctggac agaccgggtg
     2761 ctgagcgggc catcatccac gtgtccctgg ccaccacact gggagacatg aaggaccacc
     2821 atggggccgt gcgccactat gaggaggaac tgaggctgcg cagcggcaac gtgctggagg
     2881 aggccaagac ctggctgaac attgcactgt cccgcgagga ggccggcgat gcctacgagc
     2941 tgctggcccc gtgcttccag aaagcgctca gctgtgccca gcaggcccag cgtccccagc
     3001 tgcagaggca ggtcttgcag catctccata ccgtgcagct gaggctgcag ccccaggagg
     3061 cccctgagac cgaaaccaga ctgcgggagc tcagtgtagc tgaagatgaa gatgaggagg
     3121 aggaggcgga ggaggcggca gccacagcgg agagcgaagc cctggaggcc ggcgaggtgg
     3181 agctctcaga gggcgaggac gacaccgatg gcctgacccc gcagctggag gaggacgagg
     3241 agcttcaggg ccacctgggc cggcggaagg ggagcaagtg gaaccggcga aacgacatgg
     3301 gggagaccct gctgcaccga gcctgcatcg agggccagct gcgccgcgtc caggaccttg
     3361 tgaggcaggg ccaccccctt aaccctcggg actactgtgg ctggacacct ctgcacgagg
     3421 cctgcaacta cgggcatcta gaaattgtcc gcttcctgct ggaccacggg gccgcagtgg
     3481 acgacccagg tggccagggc tgcgaaggca tcacccccct ccacgatgcc ctcaactgtg
     3541 gccacttcga ggtggctgag ctgctgcttg aacggggggc gtccgtcacc ctccgcactc
     3601 gaaagggcct cagcccgctg gagacgctgc agcagtgggt gaagctgtac cgcagggacc
     3661 tggacctgga gacgcggcag aaggccaggg ccatggagat gctgctccag gcggctgcct
     3721 cgggccaaga tccccacagc tcccaggcct tccacacccc aagcagcctt ctgtttgacc
     3781 ccgagacctc tcctcctttg agcccctgcc cagaaccccc ctctaatagc actagactcc
     3841 cagaggcctc tcaggtccat gtcagggtct ccccagggca ggcggcacca gccatggcca
     3901 ggcctcggag gagcaggcat gggccagcca gcagcagcag cagctcagaa ggcgaggaca
     3961 gcgcaggccc cgcacggccg tcccagaaga ggcctcggtg ctcggccaca gcacaacggg
     4021 tggcagcctg gacgcctggc cccgccagca acagggaagc agccacagcc agcaccagcc
     4081 gggcagccta ccaggcagcc atccggggtg tgggcagtgc tcagagccgg ctggggcctg
     4141 gcccaccgcg gggccacagc aaagcccttg ccccccaggc agcgctcatc ccggaggagg
     4201 agtgcctggc tggggactgg ctggagctgg acatgcccct gacccgcagc cgccggcccc
     4261 gcccccgggg cactggagac aaccgcaggc ccagtagtac ctctgggtcg gacagtgagg
     4321 agagcaggcc ccgtgcccga gccaagcagg tccgcctgac ctgcatgcag agttgcagtg
     4381 cgccagttaa cgcagggccc agcagcctgg cttcagaacc tccagggagc cccagcaccc
     4441 ccagggtctc agagcccagt ggggacagct ctgcggcagg ccagcccttg ggtccggccc
     4501 cgccccctcc catccgggtt cgagttcaag ttcaggatca tctcttcctc atccctgtcc
     4561 cacacagcag tgacacccac tctgtggcct ggctggccga gcaggcggcc cagcgctact
     4621 accagacctg cgggctgctg cccaggctca ccctacggaa agagggggcc ctgctggccc
     4681 cacaggacct catccctgat gtgctgcaga gcaatgacga ggtgttggct gaggtgactt
     4741 cgtgggacct gcccccgttg actgaccgct accgcagggc ctgccagagc ctggggcaag
     4801 gggagcacca acaggtgctg caggccgtgg agctccaggg cttgggcctc tcgttcagcg
     4861 cctgctccct ggccctggac caggcccagc ttacacccct gctgcgggcc ctcaagctgc
     4921 acacagcact ccgggagctg cgcctggcag ggaaccggct gggggacaag tgtgtggctg
     4981 agctggtggc tgccctgggc accatgccca gcctggccct ccttgacctc tcctccaatc
     5041 acctgggtcc cgaaggcctg cgccagcttg ccatggggct cccaggccaa gccaccttgc
     5101 agagtttgga ggaattagat ctatcgatga accccctggg ggacggctgt ggccagtccc
     5161 tggcctccct cctgcacgcc tgccccttac tcagcaccct gcgcctgcag gcgtgtggct
     5221 tcggccccag cttctttctg agccaccaga cagcactggg tagtgctttc caagatgctg
     5281 agcacctgaa gaccctgtcc ctgtcctaca acgccctggg agcccctgcc ctggccagga
     5341 ccctgcagag cctgcccgcc ggcaccctcc tgcacttaga gctcagctcc gtggcagccg
     5401 gcaagggtga ttcggacctc atggagcctg tattccgata cctggccaag gaaggctgtg
     5461 ctctagccca cctgaccctg tctgcaaacc acctggggga caaggctgtt agagacctgt
     5521 gcagatgtct ctctctgtgc ccctcactca tctcactgga tctgtctgcc aaccctgaga
     5581 tcagctgtgc cagcttggaa gagctcctgt ccaccctcca aaagcggccc caaggcctta
     5641 gcttccttgg cctgtcaggc tgcgccgtcc agggtcccct gggcctgggc ctgtgggaca
     5701 agatagccgc gcagctccgg gaactgcagc tgtgcagcag acgcctctgc gctgaggaca
     5761 gggacgccct gcgccagctg cagcccagtc ggccgggccc cggcgagtgc acgctggacc
     5821 acggctccaa gctcttcttt cggcgcctct agctcgagtc tagagggccc gtttaaaccc
     5881 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     5941 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     6001 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     6061 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     6121 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     6181 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     6241 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     6301 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     6361 tcgaccccaa aaaacttgat tagggtgatg gttcacgtac ctagaagttc ctattccgaa
     6421 gttcctattc tctagaaagt ataggaactt ccttggccaa aaagcctgaa ctcaccgcga
     6481 cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct
     6541 cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc
     6601 gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat
     6661 cggccgcgct cccgattccg gaagtgcttg acattgggga gttcagcgag agcctgacct
     6721 attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc
     6781 ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc
     6841 agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg
     6901 atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca
     6961 ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc
     7021 ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg
     7081 gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg
     7141 tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact
     7201 tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca
     7261 ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg
     7321 cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa
     7381 tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg
     7441 gaaaccgacg ccccagcact cgtccgaggg caaaggaata gcacgtacta cgagatttcg
     7501 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     7561 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     7621 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7681 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     7741 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     7801 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     7861 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7921 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     7981 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     8041 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     8101 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     8161 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     8221 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     8281 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     8341 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     8401 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     8461 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     8521 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8581 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     8641 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8701 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8761 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8821 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8881 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8941 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     9001 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     9061 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     9121 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     9181 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     9241 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     9301 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     9361 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     9421 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     9481 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     9541 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9601 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9661 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9721 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9781 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9841 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9901 ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
