LOCUS       sequence-437047-        2525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-compatible Level 0 - promoter vector carrying promoter 
            MinSyn105.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Shakhova ES, Markina NM, Mitiouchkina T, Bugaeva EN, Karataeva TA, 
            Palkina KA, Fakhranurova LI, Yampolsky IV, Sarkisyan KS, Mishin AS
  TITLE     Systematic Comparison of Plant Promoters in Nicotiana spp. 
            Expression Systems.
  JOURNAL   Int J Mol Sci. 2022 Dec 6;23(23):15441. doi: 10.3390/ijms232315441.
  PUBMED    36499768
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Sci."; date: "2022-12-6"; volume: "23(23):15441. doi"; pages: " 
            10.3390/ijms232315441"
COMMENT     Sequence Label: pNK880
FEATURES             Location/Qualifiers
     source          1..2525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      242..830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     731..750
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     984..1001
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1393..1411)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1479..1583
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1805..2525,1..71)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgcgcgcaga tcagttggaa gaatttgtcc attacgtaaa aggcgagatc accaaggtag
       61 tcggcaaata actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
      121 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
      181 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
      241 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
      301 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
      361 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
      421 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
      481 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
      541 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
      601 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      661 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      721 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      781 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      841 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      901 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      961 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     1021 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atcactctgt ggtctcagga
     1081 ggcgtgtcgt tttagtgagg aaccacgtct acaaggtagg tcacgaccac tatgccatga
     1141 gctaagcaca tacgtcagta tcagcttagc aaaaccatta ttgcgtctgc acctcacatg
     1201 taggctgaag ataagataat aatgttgaag ataagaacga ccactatgcc caattagctg
     1261 acgtaaggga tgacgcacag gttgtctgca cctcacatgt actatataag gttttgctat
     1321 tcattgaaag cagtagtgac tgatttgtat atatacttga gaccacgaag tggctcttca
     1381 gtggacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     1441 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     1501 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     1561 aataatattg aaaaaggaag agtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     1621 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttttgg
     1681 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
     1741 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
     1801 catcatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
     1861 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
     1921 tggcggcctg aagccacaca gcgatattga tttgctggtt acggtgaccg taaggcttga
     1981 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
     2041 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
     2101 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
     2161 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
     2221 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
     2281 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     2341 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     2401 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     2461 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     2521 ggcct
//
