LOCUS       sequence-437051-        2525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-compatible Level 0 - promoter vector carrying promoter 
            MinSyn108.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Shakhova ES, Markina NM, Mitiouchkina T, Bugaeva EN, Karataeva TA, 
            Palkina KA, Fakhranurova LI, Yampolsky IV, Sarkisyan KS, Mishin AS
  TITLE     Systematic Comparison of Plant Promoters in Nicotiana spp. 
            Expression Systems.
  JOURNAL   Int J Mol Sci. 2022 Dec 6;23(23):15441. doi: 10.3390/ijms232315441.
  PUBMED    36499768
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Sci."; date: "2022-12-6"; volume: "23(23):15441. doi"; pages: " 
            10.3390/ijms232315441"
COMMENT     Sequence Label: pNK879
FEATURES             Location/Qualifiers
     source          1..2525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      398..986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     887..906
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1140..1157
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1549..1567)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1635..1739
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1961..2525,1..227)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
       61 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
      121 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
      181 ttgtccatta cgtaaaaggc gagatcacca aggtagtcgg caaataactg tcagaccaag
      241 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
      301 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
      361 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
      421 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
      481 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
      541 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
      601 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
      661 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
      721 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
      781 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
      841 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
      901 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
      961 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     1021 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     1081 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     1141 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     1201 gttggccgat tcattaatca ctctgtggtc tcaggaggcg tgtcgtttta gtgaggagca
     1261 agtggatgta ggtcacgacc atcactatca gcgatcttgc ctgcctttta tgacccccgc
     1321 cgatgacgcg ggaaggagga ccgatgctga ttctctctgc cgacagtggt cccaaaggtt
     1381 gtctgcacca accattattg cgtcacatgt aggctatcag cttatgacgt aagccatgac
     1441 gtctagacct ctacaaacta tataaggttt tgctattcat tgaaagcagt agtgactgat
     1501 ttgtatatat acttgagacc acgaagtggc tcttcagtgg acgaaagggc ctcgtgatac
     1561 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     1621 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     1681 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     1741 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
     1801 cggttttcat ggcttgttat gactgttttt ttttggggta cagtctatgc ctcgggcatc
     1861 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
     1921 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc
     1981 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     2041 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagcga
     2101 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     2161 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     2221 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     2281 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     2341 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     2401 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     2461 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     2521 gctta
//
