LOCUS       sequence-437059-        2538 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-compatible Level 0 - promoter vector carrying promoter 
            MinSyn110.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Shakhova ES, Markina NM, Mitiouchkina T, Bugaeva EN, Karataeva TA, 
            Palkina KA, Fakhranurova LI, Yampolsky IV, Sarkisyan KS, Mishin AS
  TITLE     Systematic Comparison of Plant Promoters in Nicotiana spp. 
            Expression Systems.
  JOURNAL   Int J Mol Sci. 2022 Dec 6;23(23):15441. doi: 10.3390/ijms232315441.
  PUBMED    36499768
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Sci."; date: "2022-12-6"; volume: "23(23):15441. doi"; pages: " 
            10.3390/ijms232315441"
COMMENT     Sequence Label: pNK887
FEATURES             Location/Qualifiers
     source          1..2538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      174..762
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     663..682
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     916..933
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1338..1356)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1424..1528
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1750..2538,1..3)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
       61 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
      121 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
      181 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
      241 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      301 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
      361 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
      421 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
      481 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
      541 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
      601 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      661 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      721 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
      781 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
      841 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
      901 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
      961 ccgcctctcc ccgcgcgttg gccgattcat taatcactct gtggtctcag gaggcgtgtc
     1021 gttttagtga gggtgggagc caccacacat gtaggctatc agcttagcaa tcgaaaggac
     1081 agtatgtgta cagggctcac tagcaagtgg ataaactggt acttgtgtat gaagataaga
     1141 taataatgtt gaagataaga tagcaagacc tctacaactg acgtaaggga tgacgcacac
     1201 tgctaggagg acatgacgta agccatgacg tctaccgatg ctgatcctat ataaggtttt
     1261 gctattcatt gaaagcagta gtgactgatt tgtatatata cttgagacca cgaagtggct
     1321 cttcagtgga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     1381 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     1441 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     1501 gcttcaataa tattgaaaaa ggaagagtat gcgctcacgc aactggtcca gaaccttgac
     1561 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     1621 tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga
     1681 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
     1741 ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt
     1801 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca
     1861 gtggatggcg gcctgaagcc acacagcgat attgatttgc tggttacggt gaccgtaagg
     1921 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct
     1981 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt
     2041 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt
     2101 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa
     2161 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt
     2221 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc
     2281 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca
     2341 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg
     2401 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat
     2461 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc attacgtaaa aggcgagatc
     2521 accaaggtag tcggcaaa
//
