LOCUS       sequence-437062-        8853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human WDR62 isoform 4. 
            N-terminal HiBiT tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8853)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 8853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-N_WDR62:1-1518
FEATURES             Location/Qualifiers
     source          1..8853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    5916..6037
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5927..5946)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(6152..6185)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        6195..6242
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6274..7068
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6328..6347)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6938..6957
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(7226..7814)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7306..7325)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7940..7988
                     /note="synthetic polyadenylation signal"
     CDS             complement(8012..8410)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      8407..8416
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(8441..8798)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8455..8590
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        8607..8798
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     8755..8775
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggtta gcgatcgcca tggtgagcgg
     1201 ctggcggctg ttcaagaaga ttagcgggag ctccggtggc tcgagcggta tggcggccgt
     1261 agggtccgga ggctatgcgc ggaacgatgc aggggagaag ctgccctctg tcatggcggg
     1321 agttccggcg cggrggggcc agtcctcccc gccccccgcc ccaccaatct gcctacggcg
     1381 gcggacgcga ctctcgacgg cctccgagga gacggtgcag aaccgggtgt cactcgagaa
     1441 ggtgcttggc atcacagccc agaacagcag tggcctaacc tgtgaccccg gcacaggcca
     1501 tgtggcctac ctggcaggct gtgtggtggt gattttggac cccaaggaga acaagcagca
     1561 gcacatcttt aacaccgcca ggaagtctct cagtgctctg gccttctccc ctgatgggaa
     1621 gtacatagtg acaggggaga atgggcatag gcctgctgtg cgcatctggg atgtggagga
     1681 gaagaatcag gtggcggaga tgctaggcca caagtatggt gtggcgtgtg tggccttctc
     1741 acccaatatg aagcacatcg tgtccatggg ctaccaacat gacatggtgc tcaacgtctg
     1801 ggactggaag aaagacatcg tagtggcctc caacaaggta tcttgtagag tcattgccct
     1861 ctccttctca gaggacagca gctattttgt cactgttggg aaccgccatg tgaggttctg
     1921 gttcttggaa gtctccactg agacaaaggt gacgagcaca gtgccccttg tagggcgctc
     1981 gggcatcctg ggcgagctgc acaacaacat cttctgtggt gtggcctgcg gtcggggccg
     2041 gatggcgggc agtaccttct gtgtgtccta ctcgggcctc ctctgccagt tcaatgagaa
     2101 gagggtgctg gagaagtgga tcaacctgaa ggtctccctg tcttcctgcc tctgtgtcag
     2161 ccaggagctc atcttctgtg gctgcacaga tgggatagtc cgcatcttcc aggcccatag
     2221 cctgcactac ctcgccaacc tgcccaagcc acactacctt ggggtagacg tggcacaggg
     2281 cctggagccc agcttcctct tccacaggaa ggcggaagca gtctacccag atacagtggc
     2341 actgaccttc gaccccatcc accagtggct gtcctgcgtg tataaggacc acagcatcta
     2401 catctgggat gtcaaggaca tcaacagagt gggcaaggtg tggtcagagc tcttccacag
     2461 ctcctacgtt tggaacgtgg aggtgtatcc tgagtttgaa gaccagagag cttgtttgcc
     2521 atcaggatcc tttctgactt gttcttcaga caacaccatt cgcttctgga acttggacag
     2581 cagccctgat tctcactggc agaaaaacat cttcagcaat accctgctga aggtcgtgta
     2641 cgtggagaat gacatccagc acctgcagga catgtcacac ttcccagacc gggggagcga
     2701 gaatgggaca cccatggatg tgaaagccgg ggtgcgggtc atgcaggtca gtcctgacgg
     2761 ccagcatttg gcttcaggcg accgaagtgg aaatctgagg atccacgagc tgcacttcat
     2821 ggacgagctg gtcaaggtgg aggcccatga tgctgaggtg ctgtgcctgg agtactccaa
     2881 gccagagacg gggctgacct tgctggcctc agccagtcgg gaccggctga tccatgtgct
     2941 gaacgtggag aagaactaca acctggagca gacgctggat gaccactcct cctccatcac
     3001 cgccatcaag ttcgctggca acagagacat ccagatgatc agctgtgggg ctgacaagag
     3061 catctacttt cgcagtgccc agcagggttc ggatggacta cactttgtcc gtacccacca
     3121 cgtagcagag aaaaccacct tgtatgacat ggacattgac atcacccaga agtacgtggc
     3181 cgtggcctgc caggaccgca atgtgagagt ctacaacact gtgaacggga agcagaagaa
     3241 gtgctacaag ggctcccagg gtgacgaagg gtccttgctg aaggtccatg tggacccctc
     3301 aggcaccttc ctggccacca gctgctctga caaaagcatc tcagtgattg acttttactc
     3361 gggcgagtgc attgccaaga tgtttggcca ttcagaaatt attaccagca tgaagttcac
     3421 ctatgactgt catcacttga tcacagtatc tggagacagc tgcgtgttca tctggcacct
     3481 gggcccggag atcaccaact gcatgaagca gcacttgcta gagattgacc accggcagca
     3541 gcagcagcac acaaatgaca agaagcggag tggccacccc aggcaggata cgtatgtgtc
     3601 cacacctagt gagattcact ccctgagccc tggagagcaa acagaggatg atctggagga
     3661 agagtgtgag ccagaagaga tgctgaagac accatccaaa gatagcttgg atccagatcc
     3721 tcgttgcctg ctaaccaacg gcaagctgcc actgtgggca aagcggctgc taggggacga
     3781 tgatgtggca gatggctcgg ccttccacgc caagcgcagc taccagcccc acggccgctg
     3841 ggcagagcgg gccggccaag agcccctcaa gaccatcctg gatgcccagg acctggattg
     3901 ctactttacc cccatgaagc ccgagagtct ggagaactcc attctggatt cactggagcc
     3961 acagagcctg gccagcctgc tgagtgagtc agagagtccc caggaagctg gccgcgggca
     4021 cccctccttc ctgccccagc agaaggaatc atctgaggcc agtgagctca tcctctactc
     4081 tctggaggca gaagtgacag tcacagggac agacagccag tattgcagga aggaggtgga
     4141 ggccgggcct ggagaccagc agggcgactc ctacctcagg gtgtcctccg acagcccaaa
     4201 ggaccagagc ccgcctgagg actcggggga gtcagaggcc gacctggagt gcagcttcgc
     4261 agccatccac tccccagctc cgcctcctga ccctgcccct cggtttgcca cgtcgctgcc
     4321 ccatttccca ggatgcgcag gtcccacaga agatgagctg tccctgcccg agggacccag
     4381 cgtccccagc agctccctac cccagactcc ggagcaggag aagttcctcc gccaccactt
     4441 tgagacactg actgagtccc cctgcagaga gctcttcccc gcagctctgg gagacgtgga
     4501 ggcctctgaa gctgaagacc acttcttcaa cccacgcctg agtatctcca cgcagttcct
     4561 ctcaagcctc cagaaggcat ccaggttcac ccataccttc cctccccggg caacccagtg
     4621 ccttgtgaag tctccagagg tcaagctcat ggaccgaggc ggaagccagc ccagagcagg
     4681 tactggctac gcctccccag acaggaccca cgtccttgct gcagggaagg ctgaagagac
     4741 cctggaggcc tggcgcccac cacctccctg ccttacgagc ctggcgtcct gtgtccctgc
     4801 ttcctccgtg ctgcccacag acaggaatct cccaacgccc acatctgcac ccaccccagg
     4861 cctggctcag ggtgtccatg ccccctccac ctgttcctac atggaggcca ctgccagctc
     4921 ccgtgccagg atatcacgca gcatctccct cggtgacagt gagggcccta tcgtggccac
     4981 actggcccag cccctccgta ggccatcgtc cgttggggag ctggcctcct tgggccagga
     5041 gcttcaggcc atcaccaccg cgacaacacc cagtttggac agtgagggcc aagagcctgc
     5101 cctgcgttcc tggggcaacc acgaggcccg ggccaacctg agactgaccc tgtcaagtgc
     5161 ctgtgatggg ctcctgctgc cccccgtgga tacccagcct ggcgtcaccg tccctgcagt
     5221 gagcttccca gcccctagcc ctgtggaaga gagcgccctg aggctccacg gctctgcctt
     5281 tcgcccaagt ctcccagctc ctgagtcccc tggccttcct gcccacccca gtaaccccca
     5341 gcttccagag gcccggcctg gcatccctgg cagcactgcc tccctcctgg agcccacctc
     5401 cggtgcactt ggtctgttcc agggcagccc tgcccgctgg agtgagccct gggtgccggt
     5461 tgaagccctg cccccatctc cccttgagct gagcagggtg gggaacatct tgcacaggct
     5521 gcagaccacc ttccaagaag ccctcgacct ttaccgtgtg ttggtctcca gtggccaggt
     5581 ggacaccggg cagcagcagg cacggactga gctggtctcc accttcctgt ggatccacag
     5641 ccagctggag gctgaatgcc tggtggggac tagtgtggcc ccagcccagg ctctgcccag
     5701 cccaggaccc ccgtccccac cgacgctgta ccccctggcc agcccagacc tgcaggccct
     5761 gctggaacac tactcggagc tgctggtgca ggccgtgcgg aggaaggcac gggggcacta
     5821 atctagaggt caagcttcgg gggtttaaac ctgcaggcat gcaagctgat ttggctgctg
     5881 ccaccgctga gcaaggccgc ttcgagcaga catgataaga tacattgatg agtttggaca
     5941 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     6001 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     6061 tatgtttcag gttcaggggg agatgtggga ggttttttta agcaagtaaa acctctacaa
     6121 atgtggtaaa atcttaatta agacttctaa tataacttcg tatagcatac attatacgaa
     6181 gttatggttc cactgttgac aattaatcat cggcatagta tatcggcata gtataatacg
     6241 acaaggtgag gaactaaacc caggaggcag atcatgattg aacaagatgg attgcacgca
     6301 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     6361 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     6421 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     6481 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     6541 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     6601 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     6661 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     6721 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     6781 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     6841 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     6901 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     6961 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     7021 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgatt cgaaatgacc
     7081 gaccaagcga cgcccaaccg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     7141 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     7201 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     7261 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     7321 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     7381 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     7441 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7501 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7561 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7621 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     7681 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     7741 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7801 aaaaaggatt tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7861 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7921 ttttatagtc cggaatcgtc acacaaaaaa ccaacacaca gatgtaatga aaataaagat
     7981 attttattgc ggccatcgtg atggctagag tttagccctc ccacacataa ccagagggca
     8041 gcaattcacg aatcccaact gccgtcggct gtccatcact gtccttcact atggctttga
     8101 tcccaggatg cagatcgaga agcacctgtc ggcaccgtcc gcaggggctc aagatgcccc
     8161 tgttctcatt tccgatcgcg acgatacaag tcaggttgcc agctgccgca gcagcagcag
     8221 tgcccagcac cacgagttct gcacaaggtc ccccagtaaa atgatataca ttgacaccag
     8281 tgaagatgcg gccgtcgcta gagagagctg cgctggcgac gctgtagtct tcagagatgg
     8341 ggatgctgtt gattgtagcc gttgctcttt caatgagggt ggattcttct tgagacaaag
     8401 gcttggccat ggtggcgcta gtgtcagaag aatcgagctt tttgcaaaag cctaggcctc
     8461 caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg
     8521 cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta
     8581 ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc
     8641 tttgcatact tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat
     8701 tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta
     8761 actgacacac attccacagc tggttctttc cgcctcagaa ggtacctaac caagttcctc
     8821 tttcagaggt tatttcaggc catggtgctg cgc
//
