LOCUS       sequence-437063-        6728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human TAF5. C-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6728)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 6728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-C_TAF5:1-800
FEATURES             Location/Qualifiers
     source          1..6728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1241..1250
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3791..3912
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3802..3821)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(4027..4060)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4070..4117
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4149..4943
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4203..4222)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4813..4832
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(5101..5689)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5181..5200)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5815..5863
                     /note="synthetic polyadenylation signal"
     CDS             complement(5887..6285)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      6282..6291
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6316..6673)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6330..6465
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        6482..6673
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6630..6650
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggcga tcgcaacaag cttcgggaag
     1201 atctggagct agcggcgggg gatccggtgg gaattcgaca ccatggcggc gctggcggag
     1261 gagcagacgg aggtggcggt caagctagag cctgagggac cgccaacgct gctacctccg
     1321 caggcggggg acggcgcagg cgagggtagc ggcggcacta ccaacaacgg ccccaacggc
     1381 ggcggcggga acgttgcggc gtcgtcgtcc actggcgggg atggcgggrc ccccaagccc
     1441 mcggtggctg tctccgccgc tgccccggcg ggggcggccc cggtgcccgc cgctgctccg
     1501 gacgccggcg ctccgcatga ccgacagact ctactggccg tgctgcagtt cctacggcag
     1561 agcaaactcc gcgaggccga agaggcgctg cgccgtgagg ccgggctgct ggaggaggca
     1621 gtggcgggct ccggagcccc gggagaggtg gacagcgccg gcgctgaggt gaccagcgcg
     1681 cttctcagcc gggtgaccgc ctcggcccct ggccctgcgg cccccgaccc tccgggcact
     1741 ggcgcttcgg gggccacggt cgtctcaggt tcagcctcag gtcctgcggc tccgggtaaa
     1801 gttggaagtg ttgctgtgga agaccagcca gatgtcagtg ccgtgttgtc agcctacaac
     1861 caacaaggag atcccacaat gtatgaagaa tactatagtg gactgaaaca cttcattgaa
     1921 tgttccctgg actgccatcg ggcagagttg tcccaacttt tttatcctct gtttgtgcac
     1981 atgtacttgg agctagtcta caatcaacat gagaatgaag caaagtcatt ctttgagaag
     2041 ttccatggag atcaggaatg ttattaccag gatgacctac gagtattatc tagtcttacc
     2101 aaaaaggaac acatgaaagg gaatgagacc atgttggatt ttcgaacaag taaatttgtt
     2161 ctgcgtattt cccgtgactc gtaccaactc ttgaagaggc atcttcagga gaaacagaac
     2221 aatcagatat ggaacatagt tcaggagcac ctctacattg acatctttga tgggatgccg
     2281 cgtagtaagc aacagataga tgcgatggtg ggaagtttgg caggagaggc taaacgagag
     2341 gcaaacaaat caaaggtatt ttttggttta ttaaaagaac cagaaattga ggtacctttg
     2401 gatgacgagg atgaagaggg agaaaatgaa gaaggaaaac ctaaaaagaa gaagcctaaa
     2461 aaagatagta ttggatccaa aagcaaaaaa caagatccca atgctccacc tcagaacaga
     2521 atccctcttc ctgagttgaa agattcagat aagttggata agataatgaa tatgaaagaa
     2581 accaccaaac gagtgcgcct tgggccggac tgcttaccct ccatttgttt ctatacattt
     2641 ctcaatgctt accagggtct cactgcagtg gatgtcactg atgattctag tctgattgct
     2701 ggaggttttg cagattcaac tgtcagagtg tggtcggtaa cacccaaaaa gcttcgtagt
     2761 gtcaaacaag catcagatct tagtcttata gacaaagaat cagatgatgt cttagaaaga
     2821 atcatggatg agaaaacagc aagtgagttg aagattttgt atggtcacag tgggcctgtc
     2881 tacggagcca gcttcagtcc ggataggaac tatctgcttt cctcttcaga ggacggaact
     2941 gttagattgt ggagccttca aacatttact tgtttggtgg gatataaagg acacaactat
     3001 ccagtatggg acacacaatt ttctccatat ggatattatt ttgtgtcagg gggccatgac
     3061 cgagtagctc ggctctgggc tacagaccac tatcagcctt taagaatatt tgccggccat
     3121 cttgctgatg tgaattgtac cagattccat ccaaattcta attatgttgc tacgggctct
     3181 gcagacagaa ctgtgcggct ctgggacgtc ctgaatggta actgtgtaag gatcttcact
     3241 ggacacaagg gaccaattca ttccttgaca ttttctccca atgggagatt cctggctaca
     3301 ggagcaacag atggcagagt gcttctttgg gatattggac atggtttgat ggttggagaa
     3361 ttaaaaggcc acactgatac agtctgttca cttaggttta gtagagatgg tgaaattttg
     3421 gcatcaggtt caatggataa tacagttcga ttatgggatg ctatcaaagc ctttgaagat
     3481 ttagagaccg atgactttac tacagccact gggcatataa atttacctga gaattcacag
     3541 gagttattgt tgggaacata tatgaccaaa tcaacaccag ttgtacacct tcattttact
     3601 cgaagaaacc tggttctagc tgcaggagct tatagtccac aaggctcgag cggtgggagc
     3661 tccggtgtga gcggctggcg gctgttcaag aagattagct aagtttaaac ggccgcgact
     3721 ctagactgca ggcatgcaag ctgatttggc tgctgccacc gctgagcaag gccgcttcga
     3781 gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     3841 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     3901 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg
     3961 tgggaggttt ttttaagcaa gtaaaacctc tacaaatgtg gtaaaatctt aattaagact
     4021 tctaatataa cttcgtatag catacattat acgaagttat ggttccactg ttgacaatta
     4081 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaacccagga
     4141 ggcagatcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     4201 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     4261 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     4321 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     4381 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     4441 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     4501 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     4561 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     4621 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     4681 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     4741 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     4801 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     4861 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     4921 gccttcttga cgagttcttc tgattcgaaa tgaccgacca agcgacgccc aaccggtatc
     4981 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     5041 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     5101 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     5161 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     5221 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     5281 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     5341 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     5401 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     5461 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     5521 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     5581 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     5641 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatttcaag aagatccttt
     5701 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     5761 catgagatta tcaaaaagga tcttcaccta gatcctttta tagtccggaa tcgtcacaca
     5821 aaaaaccaac acacagatgt aatgaaaata aagatatttt attgcggcca tcgtgatggc
     5881 tagagtttag ccctcccaca cataaccaga gggcagcaat tcacgaatcc caactgccgt
     5941 cggctgtcca tcactgtcct tcactatggc tttgatccca ggatgcagat cgagaagcac
     6001 ctgtcggcac cgtccgcagg ggctcaagat gcccctgttc tcatttccga tcgcgacgat
     6061 acaagtcagg ttgccagctg ccgcagcagc agcagtgccc agcaccacga gttctgcaca
     6121 aggtccccca gtaaaatgat atacattgac accagtgaag atgcggccgt cgctagagag
     6181 agctgcgctg gcgacgctgt agtcttcaga gatggggatg ctgttgattg tagccgttgc
     6241 tctttcaatg agggtggatt cttcttgaga caaaggcttg gccatggtgg cgctagtgtc
     6301 agaagaatcg agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
     6361 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     6421 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     6481 cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     6541 gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct
     6601 gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc acagctggtt
     6661 ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt caggccatgg
     6721 tgctgcgc
//
