LOCUS       sequence-437067-        2590 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-compatible Level 0 - promoter vector carrying promoter 
            MinSyn159x.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Shakhova ES, Markina NM, Mitiouchkina T, Bugaeva EN, Karataeva TA, 
            Palkina KA, Fakhranurova LI, Yampolsky IV, Sarkisyan KS, Mishin AS
  TITLE     Systematic Comparison of Plant Promoters in Nicotiana spp. 
            Expression Systems.
  JOURNAL   Int J Mol Sci. 2022 Dec 6;23(23):15441. doi: 10.3390/ijms232315441.
  PUBMED    36499768
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Sci."; date: "2022-12-6"; volume: "23(23):15441. doi"; pages: " 
            10.3390/ijms232315441"
COMMENT     Sequence Label: pNK882
FEATURES             Location/Qualifiers
     source          1..2590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      465..1053
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     954..973
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1207..1224
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1681..1699)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1767..1871
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2093..2590,1..294)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
       61 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
      121 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
      181 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
      241 gaagaatttg tccattacgt aaaaggcgag atcaccaagg tagtcggcaa ataactgtca
      301 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
      361 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
      421 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
      481 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
      541 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
      601 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
      661 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
      721 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
      781 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
      841 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
      901 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
      961 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     1021 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     1081 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     1141 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     1201 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     1261 cccgcgcgtt ggccgattca ttaatcactc tgtggtctca ggaggcgtgt cgttttagtg
     1321 aggatgacgt aagccatgac gtctagctag gaggaccgat gctgatcttg cctatcgaaa
     1381 ggacagtaac ttgtgtacag gtggtggagc acgacaggta cttgtgtaca gggctcgtgg
     1441 gagccaccac gatgctgaca gccacttgtg tattaggttg tctgcacctc acatgtaggc
     1501 ttgaagcatc ttcctcagct tagcaagacc tctacaaaac tggtctgacg taagggatga
     1561 cgcacataca gggctcactg ctaggaggac tatataaggt tttgctattc attgaaagca
     1621 gtagtgactg atttgtatat atacttgaga ccacgaagtg gctcttcagt ggacgaaagg
     1681 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     1741 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     1801 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     1861 aaaggaagag tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     1921 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttttgggg tacagtctat
     1981 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
     2041 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatgaggga
     2101 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
     2161 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
     2221 gccacacagc gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
     2281 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
     2341 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
     2401 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
     2461 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
     2521 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
     2581 tgaggcgcta
//
