LOCUS       sequence-437070-        2567 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-compatible Level 0 - promoter vector carrying promoter 
            MinSyn1569x.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Shakhova ES, Markina NM, Mitiouchkina T, Bugaeva EN, Karataeva TA, 
            Palkina KA, Fakhranurova LI, Yampolsky IV, Sarkisyan KS, Mishin AS
  TITLE     Systematic Comparison of Plant Promoters in Nicotiana spp. 
            Expression Systems.
  JOURNAL   Int J Mol Sci. 2022 Dec 6;23(23):15441. doi: 10.3390/ijms232315441.
  PUBMED    36499768
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Sci."; date: "2022-12-6"; volume: "23(23):15441. doi"; pages: " 
            10.3390/ijms232315441"
COMMENT     Sequence Label: pNK884
FEATURES             Location/Qualifiers
     source          1..2567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      182..770
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     671..690
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     924..941
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1375..1393)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1461..1565
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1787..2567,1..11)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tcggcaaata actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
       61 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
      121 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
      181 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
      241 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
      301 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
      361 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
      421 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
      481 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
      541 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      601 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      661 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      721 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      781 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      841 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      901 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
      961 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atcactctgt ggtctcagga
     1021 ggcgtgtcgt tttagtgagg ctgacgtaag ggatgacgca caaggctatc agcttagcaa
     1081 gacctctaca aaaatttcgg gaaacctcct cgagggctca ctgctaggag gaccgatgaa
     1141 ccattattgc gtgtaggcta tcagcttagt ccatcaacaa ataatccaag taagtatcaa
     1201 atatttcttg tggaccgatg ctgatcttgc ctgccttgat gatgagctaa gcacatacgt
     1261 cagtacttgt gtacagggct cacctatata aggttttgct attcattgaa agcagtagtg
     1321 actgatttgt atatatactt gagaccacga agtggctctt cagtggacga aagggcctcg
     1381 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     1441 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     1501 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     1561 agagtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     1621 cagtggcggt tttcatggct tgttatgact gttttttttt ggggtacagt ctatgcctcg
     1681 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     1741 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatga gggaagcggt
     1801 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     1861 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     1921 cagcgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     1981 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     2041 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     2101 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     2161 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     2221 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     2281 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     2341 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     2401 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     2461 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     2521 gttggaagaa tttgtccatt acgtaaaagg cgagatcacc aaggtag
//
