LOCUS       sequence-437072-        2631 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-compatible Level 0 - promoter vector carrying promoter 
            MinSyn1824x.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Shakhova ES, Markina NM, Mitiouchkina T, Bugaeva EN, Karataeva TA, 
            Palkina KA, Fakhranurova LI, Yampolsky IV, Sarkisyan KS, Mishin AS
  TITLE     Systematic Comparison of Plant Promoters in Nicotiana spp. 
            Expression Systems.
  JOURNAL   Int J Mol Sci. 2022 Dec 6;23(23):15441. doi: 10.3390/ijms232315441.
  PUBMED    36499768
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Sci."; date: "2022-12-6"; volume: "23(23):15441. doi"; pages: " 
            10.3390/ijms232315441"
COMMENT     Sequence Label: pNK885
FEATURES             Location/Qualifiers
     source          1..2631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      116..704
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     605..624
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     858..875
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1373..1391)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1459..1563
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1785..2576
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
       61 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
      121 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
      181 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
      241 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
      301 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
      361 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      421 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
      481 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
      541 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      601 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      661 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
      721 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
      781 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
      841 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
      901 aaccgcctct ccccgcgcgt tggccgattc attaatcact ctgtggtctc aggaggcgtg
      961 tcgttttagt gaggctgacg taagggatga cgcacatgta ggctatcagc ttagcaaaga
     1021 ttgatgaaaa gtcaaaaaca aaaatcaatt atatcttgcc tgccttgatg aggaaaaaga
     1081 agaggtcaag acctctacaa aactggtact tcactatcag caggctatca gctttctctc
     1141 tgccgacagt ggtcccaaat acaaaactgg tacttgtgta cagggccagt ggtccctcca
     1201 catccatcaa caaataatcc aagtaaggag gaccgaatga gctaagcaca tacgtcagcg
     1261 cggtaggtca cgaccactat gctatataag gttttgctat tcattgaaag cagtagtgac
     1321 tgatttgtat atatacttga gaccacgaag tggctcttca gtggacgaaa gggcctcgtg
     1381 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     1441 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     1501 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     1561 agtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
     1621 gtggcggttt tcatggcttg ttatgactgt ttttttttgg ggtacagtct atgcctcggg
     1681 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     1741 atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgagg gaagcggtga
     1801 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     1861 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     1921 gcgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     1981 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     2041 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     2101 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     2161 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     2221 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     2281 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     2341 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     2401 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     2461 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     2521 tggaagaatt tgtccattac gtaaaaggcg agatcaccaa ggtagtcggc aaataactgt
     2581 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt t
//
