LOCUS       sequence-437075-        2620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-compatible Level 0 - promoter vector carrying promoter 
            MinSyn1904x.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Shakhova ES, Markina NM, Mitiouchkina T, Bugaeva EN, Karataeva TA, 
            Palkina KA, Fakhranurova LI, Yampolsky IV, Sarkisyan KS, Mishin AS
  TITLE     Systematic Comparison of Plant Promoters in Nicotiana spp. 
            Expression Systems.
  JOURNAL   Int J Mol Sci. 2022 Dec 6;23(23):15441. doi: 10.3390/ijms232315441.
  PUBMED    36499768
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Sci."; date: "2022-12-6"; volume: "23(23):15441. doi"; pages: " 
            10.3390/ijms232315441"
COMMENT     Sequence Label: pNK878
FEATURES             Location/Qualifiers
     source          1..2620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      343..931
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     832..851
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1085..1102
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1589..1607)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1675..1779
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2001..2620,1..172)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
       61 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
      121 agaatttgtc cattacgtaa aaggcgagat caccaaggta gtcggcaaat aactgtcaga
      181 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
      241 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
      301 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
      361 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
      421 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
      481 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
      541 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
      601 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
      661 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
      721 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
      781 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
      841 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
      901 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
      961 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     1021 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     1081 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     1141 cgcgcgttgg ccgattcatt aatcactctg tggtctcagg aggcgtgtcg ttttagtgag
     1201 gatgacgtaa gccatgacgt ctaggctatc agcttagcgg aaaaagaaga ggtactatgc
     1261 ccaattaggt tgtctatcga aaggacagta cgcggtaggt cacgcaaata tttcttgttt
     1321 tatgaccccc gccgatgacg cgggagtaca gtggtggagc acgacaggct atcaaatttc
     1381 gggaaacctc ctcggatctt gcctgccttg attctctctg ccgacagtgg tcccaaatgc
     1441 ctgccttgat atgagctaag cacatacgtc agcacctcac atgtaggcta tcagcttcta
     1501 tataaggttt tgctattcat tgaaagcagt agtgactgat ttgtatatat acttgagacc
     1561 acgaagtggc tcttcagtgg acgaaagggc ctcgtgatac gcctattttt ataggttaat
     1621 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     1681 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     1741 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgcgctcacg caactggtcc
     1801 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat
     1861 gactgttttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     1921 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     1981 ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat
     2041 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     2101 acggctccgc agtggatggc ggcctgaagc cacacagcga tattgatttg ctggttacgg
     2161 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     2221 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     2281 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     2341 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     2401 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     2461 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     2521 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     2581 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
//
