LOCUS       sequence-437079-        2556 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-compatible Level 0 - promoter + 5U vector carrying promoter 
            AtTCTP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Shakhova ES, Markina NM, Mitiouchkina T, Bugaeva EN, Karataeva TA, 
            Palkina KA, Fakhranurova LI, Yampolsky IV, Sarkisyan KS, Mishin AS
  TITLE     Systematic Comparison of Plant Promoters in Nicotiana spp. 
            Expression Systems.
  JOURNAL   Int J Mol Sci. 2022 Dec 6;23(23):15441. doi: 10.3390/ijms232315441.
  PUBMED    36499768
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Sci."; date: "2022-12-6"; volume: "23(23):15441. doi"; pages: " 
            10.3390/ijms232315441"
COMMENT     Sequence Label: pNK875
FEATURES             Location/Qualifiers
     source          1..2556
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      414..1002
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     903..922
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1156..1173
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1596..1614)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1682..1786
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2008..2556,1..243)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
       61 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
      121 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
      181 gatcagttgg aagaatttgt ccattacgta aaaggcgaga tcaccaaggt agtcggcaaa
      241 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
      301 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
      361 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
      421 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
      481 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      541 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
      601 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
      661 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
      721 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
      781 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
      841 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      901 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      961 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     1021 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     1081 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     1141 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     1201 ccgcctctcc ccgcgcgttg gccgattcat taatcactct gtggtctcag gagccaacac
     1261 tcgaatcccc acccgttgga ccaaacccgg ctcattaagc gtcggttcag atttatttcc
     1321 tttatttaaa aaaaaggaaa gggtaaaaaa tagaaaattg gaaacagtta aagcccaaaa
     1381 ttgtaattta ccgagaattg taaatttacc tgaaaaccct acgctatagt ttcgactata
     1441 aataccaaac ttaggacctc acttcagaat cccctcgtcg ctgcgtctct ctcccgcaac
     1501 cttcgatttt cgtttattcg catccatcgg agagagaaaa caatcaataa gcgaccaatg
     1561 tgagaccacg aagtggctct tcagtggacg aaagggcctc gtgatacgcc tatttttata
     1621 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     1681 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     1741 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatgc gctcacgcaa
     1801 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     1861 ttgttatgac tgtttttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
     1921 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     1981 agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact
     2041 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     2101 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagcgatat tgatttgctg
     2161 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     2221 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     2281 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     2341 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     2401 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     2461 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     2521 ctatggaact cgccgcccga ctgggctggc gatgag
//
