LOCUS       sequence-437087-        2708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-compatible Level 0 - promoter + 5U vector carrying promoter 
            AtPD7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Shakhova ES, Markina NM, Mitiouchkina T, Bugaeva EN, Karataeva TA, 
            Palkina KA, Fakhranurova LI, Yampolsky IV, Sarkisyan KS, Mishin AS
  TITLE     Systematic Comparison of Plant Promoters in Nicotiana spp. 
            Expression Systems.
  JOURNAL   Int J Mol Sci. 2022 Dec 6;23(23):15441. doi: 10.3390/ijms232315441.
  PUBMED    36499768
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Sci."; date: "2022-12-6"; volume: "23(23):15441. doi"; pages: " 
            10.3390/ijms232315441"
COMMENT     Sequence Label: pNK869
FEATURES             Location/Qualifiers
     source          1..2708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      258..846
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     747..766
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1000..1017
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1592..1610)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1678..1782
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2004..2708,1..87)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc
       61 gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag
      121 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
      181 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
      241 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
      301 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
      361 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
      421 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
      481 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
      541 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
      601 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
      661 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
      721 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
      781 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
      841 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
      901 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
      961 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     1021 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatca
     1081 ctctgtggtc tcaggagata ggcaaccgtg gacttcttca ctagctcatc gagatagcaa
     1141 tgccactagc taatttctta cgttgtatct tatttgtttt actttggggc atgacatggt
     1201 taaaccccat caaagagaaa gtgtttcatc cactagtcaa tatacgaatt cattcgaatt
     1261 tatccctgta aatcctagtt cttaggatga actggtgtaa taaacagcaa aaaaaaaaaa
     1321 aaaaaaaaaa acctggaatc attcgaccac ctcaataaac taaagctacc aattaccaca
     1381 atatagtctt tcatatccac ttagatataa aagataaaag taaacaaata ttaaatttca
     1441 tatgcacgca taggaaactc atgatcttat cttttaaata gacatctagt tttcttaggt
     1501 tataaataga cattttgtcc tagaacttct tcactactaa aacctagcct caaatgtgag
     1561 accacgaagt ggctcttcag tggacgaaag ggcctcgtga tacgcctatt tttataggtt
     1621 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     1681 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1741 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgcgctc acgcaactgg
     1801 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
     1861 tatgactgtt tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     1921 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     1981 gccctaaaac aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     2041 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     2101 tgtacggctc cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta
     2161 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     2221 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     2281 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     2341 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     2401 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     2461 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     2521 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     2581 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     2641 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     2701 gacaagaa
//
