LOCUS       sequence-437092-        2511 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-compatible Level 0 - 3U + terminator vector carrying 
            terminator HSP18.2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Shakhova ES, Markina NM, Mitiouchkina T, Bugaeva EN, Karataeva TA, 
            Palkina KA, Fakhranurova LI, Yampolsky IV, Sarkisyan KS, Mishin AS
  TITLE     Systematic Comparison of Plant Promoters in Nicotiana spp. 
            Expression Systems.
  JOURNAL   Int J Mol Sci. 2022 Dec 6;23(23):15441. doi: 10.3390/ijms232315441.
  PUBMED    36499768
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Sci."; date: "2022-12-6"; volume: "23(23):15441. doi"; pages: " 
            10.3390/ijms232315441"
COMMENT     Sequence Label: pNK1198
FEATURES             Location/Qualifiers
     source          1..2511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      278..866
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     767..786
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1020..1037
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      1127..1375
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(1415..1433)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1501..1605
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1827..2511,1..107)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
       61 ttgtccatta cgtaaaaggc gagatcacca aggtagtcgg caaataactg tcagaccaag
      121 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
      181 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
      241 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
      301 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
      361 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
      421 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
      481 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
      541 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
      601 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
      661 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
      721 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
      781 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
      841 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
      901 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
      961 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     1021 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     1081 gttggccgat tcattaatca ctctgtggtc tcagctttag gttaaatatg aagatgaaga
     1141 tgaaatattt ggtgtgtcaa ataaaaagct tgtgtgctta agtttgtgtt tttttcttgg
     1201 cttgttgtgt tatgaatttg tggctttttc taatattaaa tgaatgtaag atctcattat
     1261 aatgaataaa caaatgtttc tataatccat tgtgaatgtt ttgttggatc tcttctgcag
     1321 catataacta ctgtatgtgc tatggtatgg actatggaat atgattaaag ataagcgctt
     1381 gagaccacga agtggctctt cagtggacga aagggcctcg tgatacgcct atttttatag
     1441 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     1501 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     1561 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgcg ctcacgcaac
     1621 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct
     1681 tgttatgact gttttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     1741 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     1801 gtcgccctaa aacaaagtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc
     1861 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     1921 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagcgatatt gatttgctgg
     1981 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     2041 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     2101 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     2161 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     2221 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     2281 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     2341 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     2401 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     2461 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga c
//
