LOCUS       sequence-437105-        3515 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-compatible Level 0 promoterless vector encoding mutant of 
            Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised 
            for expression in Nicotiana benthamiana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Shakhova ES, Karataeva TA, Markina NM, Mitiouchkina T, Palkina KA, 
            Perfilov MM, Wood MG, Hoang TT, Hall MP, Fakhranurova LI, Alekberova
            AE, Malyshevskaia AK, Gorbachev DA, Bugaeva EN, Pletneva LK, Babenko
            VV, Boldyreva DI, Gorokhovatsky AY, Balakireva AV, Gao F, Choob VV, 
            Encell LP, Wood KV, Yampolsky IV, Sarkisyan KS, Mishin AS
  TITLE     An improved pathway for autonomous bioluminescence imaging in 
            eukaryotes.
  JOURNAL   Nat Methods. 2024 Mar;21(3):406-410. doi: 
            10.1038/s41592-023-02152-y. Epub 2024 Jan 22.
  PUBMED    38253843
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-023-02152-y"; journalName: "Nat Methods"; date: 
            "2024-03"; volume: "21"; issue: "3"; pages: "406-410"
COMMENT     Sequence Label: pNK116
FEATURES             Location/Qualifiers
     source          1..3515
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      288..876
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     777..796
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1030..1047
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2429..2447)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2515..2619
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2841..3515,1..117)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag
       61 ttggaagaat ttgtccatta cgtaaaaggc gagatcacca aggtagtcgg caaataactg
      121 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
      181 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
      241 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
      301 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
      361 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
      421 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
      481 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
      541 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
      601 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
      661 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
      721 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
      781 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
      841 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
      901 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
      961 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     1021 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     1081 ctccccgcgc gttggccgat tcattaatca ctctgtggtc tcaaatggcc tcttttgaaa
     1141 attcactctc tgtgttgatc gtgggagccg gtttgggtgg tctagcagct gctatcgctc
     1201 tccgacgaca gggccacgtg gttaagatct acgaatcatc tagctttaag gccgagctcg
     1261 gcgcaggctt ggcagttcca ccgaatacac tgcgtagtct acagcaattg ggttgtaaca
     1321 ctgaaaacct taacggcgtt gataatctct gttttactgc catgggatat gatgggtcag
     1381 tgggcatgat gaacaatatg actgattacc gtgaagcata tggcactagt tggatcatgg
     1441 ttcatcgtgt cgatttgcat aacgagttga tgagagttgc tttggaccca gggggattgg
     1501 gccctcccgc aacgcttcac ctgaatcatc gagtcacgtt ttgtgatgta gatgcttgta
     1561 cggtgacatt taccaacggt acaactcaaa gtgcagatct tatcgttggg gctgacggga
     1621 tacgttcaac tatccgtaga tttgtcttgg aggaggacgt gaccattccc gcaagcggaa
     1681 ttgtggggtt tcgatggttg gtacaggctg atgccctcga tccatatcct gaactcgatt
     1741 ggatagtcaa aaagccaccg ctcggagcta ggttgatttc tacgccccag aatccacaat
     1801 ctggagtggg actggcagat cgtagaacta ttattatata cgcttgtcgt ggggggacaa
     1861 tggtaaatgt gctggccgta cacgatgacg agcgagatca gaatactgca gattggagcg
     1921 ttccagcatc aaaggatgac ttgtttcgag tattccatga ctaccatccg agattcagac
     1981 ggctcttgga attggctcaa gatataaatc tctggcagat gagggttgtg ccggtgctca
     2041 agaaatgggt gaacaaacga gtgtgcctgc ttggagatgc cgctcatgcc atgcttccga
     2101 ctctcggcca gggtttcggg atgggattgg aggacgccgt agcccttggt actctcttgc
     2161 caaaaggtac gactgctagt caaatagaga caagactggc tgtctacgaa cagcttagaa
     2221 aggaccgagc agagtttgta gccgctgaaa gttatgagga gcagtatgtc cccgagaaga
     2281 ggggcttgta cttgcgtagc aaggaactta gagatagagt catggggtat gacataaaag
     2341 tggagtctga gaaggtactg gaaaccttgc ttaggagctc taactcagca ggttgagacc
     2401 acgaagtggc tcttcagtgg acgaaagggc ctcgtgatac gcctattttt ataggttaat
     2461 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     2521 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     2581 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgcgctcacg caactggtcc
     2641 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat
     2701 gactgttttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     2761 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     2821 ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat
     2881 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     2941 acggctccgc agtggatggc ggcctgaagc cacacagcga tattgatttg ctggttacgg
     3001 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     3061 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     3121 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     3181 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     3241 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     3301 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     3361 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     3421 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     3481 agcgcctgcc ggcccagtat cagcccgtca tactt
//
