LOCUS       sequence-437121-        9676 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  control plasmid-part B of SPARK-OFF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9676)
  AUTHORS   Chung CI, Yang J, Yang X, Liu H, Ma Z, Szulzewsky F, Holland EC, 
            Shen Y, Shu X
  TITLE     Phase separation of YAP-MAML2 differentially regulates the 
            transcriptome.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Feb 13;121(7):e2310430121. doi: 
            10.1073/pnas.2310430121. Epub 2024 Feb 5.
  PUBMED    38315854
REFERENCE   2  (bases 1 to 9676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-02-13"; volume: "121(7):e2310430121. 
            doi"; pages: " 10.1073/pnas.2310430121. Epub 2024 Feb 5"
COMMENT     Sequence Label: NLS-mCherry
FEATURES             Location/Qualifiers
     source          1..9676
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    65..199
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(102..121)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     156..175
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             367..1000
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1047..1172
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1669..1902
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2087..2131
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2416..2533
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2686..3093
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3111..3137
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             3138..3845
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(3280..3298)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3548..3567
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3906..4494
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3959..3979)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4377..4388)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4497..4513)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4498..4514)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4604..4837
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4862..4880)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4863..4880)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5170..5189)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5403..5422)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5522..5544
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5582..5600)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5668..5772
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5773..5841,5842..6633)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5991..6010)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6804..7392
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7293..7312
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7546..7563
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7633..7653)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7638..7967
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7818..7953
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9101..9166
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9296..9316
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
       61 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
      121 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
      181 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
      241 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga
      301 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt
      361 agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct
      421 accacacaca aggctacttc cctgattagc agaactacac accagggcca ggggtcagat
      481 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag
      541 aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg
      601 acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg
      661 cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac
      721 tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc
      781 tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag
      841 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
      901 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
      961 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     1021 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     1081 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     1141 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     1201 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     1261 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     1321 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     1381 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     1441 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     1501 cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     1561 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     1621 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     1681 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     1741 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     1801 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     1861 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     1921 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     1981 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     2041 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     2101 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     2161 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     2221 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     2281 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     2341 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc gccaaatggc
     2401 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     2461 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     2521 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttgga tcgataagct
     2581 tgatatcgaa ttcctgcagc cccgataaaa taaaagattt tatttagtct ccagaaaaag
     2641 gggggaatga aagaccccac ctgtaggttt ggcaagctag ctgcagtaac gccattttgc
     2701 aaggcatgga aaaataccaa accaagaata gagaagttca gatcaagggc gggtacatga
     2761 aaatagctaa cgttgggcca aacaggatat ctgcggtgag cagtttcggc cccggcccgg
     2821 ggccaagaac agatggtcac cgcagtttcg gccccggccc gaggccaaga acagatggtc
     2881 cccagatatg gcccaaccct cagcagtttc ttaagaccca tcagatgttt ccaggctccc
     2941 ccaaggacct gaaatgaccc tgcgccttat ttgaattaac caatcagcct gcttctcgct
     3001 tctgttcgcg cgcttctgct tcccgagctc tataaaagag ctcacaaccc ctcactcggc
     3061 gcgccagtcc tccgacagac tgagtcgccc gggggggatc cgccaccatg cccgccgcca
     3121 agcgggtgaa gctggacgtg agcaagggcg aggaggataa catggccatc atcaaggagt
     3181 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     3241 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     3301 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
     3361 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
     3421 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
     3481 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     3541 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
     3601 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
     3661 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     3721 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
     3781 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
     3841 agtaaggcgg ccgcgactct agagtcgacc tgcaggcatg caagcttgat atcaagctta
     3901 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     3961 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     4021 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     4081 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     4141 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     4201 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     4261 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     4321 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     4381 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     4441 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     4501 accgtcgacc tcgagggaat taattcgagc tcggtacctt taagaccaat gacttacaag
     4561 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     4621 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     4681 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     4741 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     4801 ctcagaccct tttagtcagt gtggaaaatc tctagcagca tctagaatta attccgtgta
     4861 ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat taattgtagc
     4921 cgcgttctaa cgacaatatg tacaagccta attgtgtagc atctggctta ctgaagcaga
     4981 ccctatcatc tctctcgtaa actgccgtca gagtcggttt ggttggacga accttctgag
     5041 tttctggtaa cgccgtcccg cacccggaaa tggtcagcga accaatcagc agggtcatcg
     5101 ctagccagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
     5161 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
     5221 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
     5281 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
     5341 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaatg gtgcactctc
     5401 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     5461 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     5521 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     5581 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     5641 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     5701 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     5761 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     5821 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     5881 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     5941 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     6001 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     6061 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     6121 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     6181 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     6241 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     6301 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     6361 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     6421 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     6481 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     6541 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     6601 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     6661 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6721 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6781 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6841 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6901 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     6961 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7021 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     7081 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7141 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7201 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7261 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7321 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7381 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     7441 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     7501 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     7561 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     7621 taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     7681 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     7741 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     7801 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     7861 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     7921 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttggacaca
     7981 agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt
     8041 gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat
     8101 ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca
     8161 cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa
     8221 ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc
     8281 ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt
     8341 gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag
     8401 gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt actgcggttg
     8461 cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc
     8521 gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatt gaacagccgt
     8581 gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg
     8641 cactgccggg cgttgttctt tttaacttca ggcgggttac aatagtttcc agtaagtatt
     8701 ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt
     8761 aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg
     8821 cagtcggccc ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg
     8881 tggatctggc gcggcattga cccacgcgaa atcctcgacg tccaggcacg tattgtgatg
     8941 agcgatgccg aacgtaccga cgatgattta tacgatacgg tgattggcta ccgtggcggc
     9001 aactggattt atgagtgggc cccggatctt tgtgaaggaa ccttacttct gtggtgtgac
     9061 ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag
     9121 tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg
     9181 aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga
     9241 agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa
     9301 aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag
     9361 tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa
     9421 agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca
     9481 taacagttat aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc
     9541 tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa
     9601 taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg
     9661 tagaggtttt acttgc
//
