LOCUS       sequence-437122-        7364 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RFP fused TEAD4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7364)
  AUTHORS   Chung CI, Yang J, Yang X, Liu H, Ma Z, Szulzewsky F, Holland EC, 
            Shen Y, Shu X
  TITLE     Phase separation of YAP-MAML2 differentially regulates the 
            transcriptome.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Feb 13;121(7):e2310430121. doi: 
            10.1073/pnas.2310430121. Epub 2024 Feb 5.
  PUBMED    38315854
REFERENCE   2  (bases 1 to 7364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-02-13"; volume: "121(7):e2310430121. 
            doi"; pages: " 10.1073/pnas.2310430121. Epub 2024 Feb 5"
COMMENT     Sequence Label: mKO3-TEAD4
FEATURES             Location/Qualifiers
     source          1..7364
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(155..259)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(334..353)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        525..904
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        905..1108
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1059..1079
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1153..1172
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1153..1171
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1207..1216
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1213..1866
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQFKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     primer_bind     complement(3245..3262)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3251..3475
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3521..3949
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3608..3627)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3818..3839
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3958..3978)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3963..4292
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4143..4278
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4205..4224
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4359..5153
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4413..4432)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5023..5042
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5327..5448
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5364..5383)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5418..5437
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5497..5513)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5497..5513)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5510..5532)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5521..5537
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5545..5551,5552..5569,5570..5575))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5590..5611
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5728..5745)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5899..6487)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5979..5998)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6658..7364,1..85,86..154))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7281..7300
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
       61 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
      121 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
      181 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
      241 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
      301 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
      361 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
      421 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
      481 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
      541 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
      601 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      661 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      721 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      781 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      841 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      901 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      961 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     1021 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     1081 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
     1141 gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc gtttaaactt
     1201 aagcttgcca ccatggtgag tgtgattaaa ccagagatga agatgaggta ctacatggac
     1261 ggctccgtca atgggcatga gttcacaatt gaaggtgaag gcacaggcag accttacgag
     1321 ggacatcaag agatgacact acgcgtcaca atggccgagg gcgggccaat gcctttcgcg
     1381 tttgacttag tgtcacacgt gttctgttac ggccacagag tatttactaa atatccagaa
     1441 gagataccag actatttcaa acaagcattt cctgaaggcc tgtcatggga aaggtcgttg
     1501 gagttcgaag atggtgggtc cgcttcagtc agtgcgcata taagccttag aggaaacacc
     1561 ttctaccaca aatccaaatt tactggggtt aactttcctg ccgatggtcc tatcatgcaa
     1621 aaccaaagtg ttgattggga gccatcaacc gagaaaatta ctgccagcga cggagttctg
     1681 aagggtgatg ttacgatgta cctaaaactt gaaggaggcg gcaatcacaa atgccaattc
     1741 aagactactt acaaggcggc aaaagagatt cttgaaatgc caggagacca ttacatcggc
     1801 catcgcctcg tcaggaaaac cgaaggcaac attactgagc aggtagaaga tgcagtagct
     1861 catagcctcg agtccggact cagatctggc agcggtggag gcgatatctt ggagggcacg
     1921 gccggcacca ttacctccaa cgagtggagc tctcccacct cccctgaggg gagcaccgcc
     1981 tctgggggca gtcaggcact ggacaagccc atcgacaatg acgcagaggg cgtgtggagc
     2041 ccggatattg agcagagttt ccaggaggcc ctcgccatct acccgccctg tggcaggcgc
     2101 aaaatcatcc tgtcggacga gggcaagatg tatggtcgga acgagctgat tgcccgctac
     2161 atcaagctcc ggacagggaa gacccgcacc aggaagcagg tctccagcca catccaggtg
     2221 ctggctcgtc gcaaagctcg cgagatccag gccaagctaa aggaccaggc agctaaggac
     2281 aaggccctgc agagcatggc tgccatgtcg tctgcacaga tcatctccgc cacggccttc
     2341 cacagtagca tggccctcgc ccggggcccc ggccgcccag cagtctcagg gttttggcaa
     2401 ggagctttgc caggccaagc cggaacgtcc catgatgtga agcctttctc tcagcaaacc
     2461 tatgctgtcc agcctccgct gcctctgcca gggtttgagt ctcctgcagg gcccgcccca
     2521 tcgccctctg cgcccccggc acccccatgg cagggccgca gcgtggccag ctccaagctc
     2581 tggatgttgg agttctctgc cttcctggag cagcagcagg acccggacac gtacaacaag
     2641 cacctgttcg tgcacattgg ccagtccagc ccaagctaca gcgaccccta cctcgaagcc
     2701 gtggacatcc gccaaatcta tgacaaattc ccggagaaaa agggtggact caaggatctc
     2761 ttcgaacggg gaccctccaa tgcctttttt cttgtgaagt tctgggcaga cctcaacacc
     2821 aacatcgagg atgaaggcag ctccttctat ggggtctcca gccagtatga gagccccgag
     2881 aacatgatca tcacctgctc cacgaaggtc tgctctttcg gcaagcaggt ggtggagaaa
     2941 gttgagacag agtatgctcg ctatgagaat ggacactact cttaccgcat ccaccggtcc
     3001 ccgctctgtg agtacatgat caacttcatc cacaagctca agcacctccc tgagaagtac
     3061 atgatgaaca gcgtgctgga gaacttcacc atcctgcagg tggtcaccaa cagagacaca
     3121 caggagacct tgctgtgcat tgcctatgtc tttgaggtgt cagccagtga gcacggggct
     3181 cagcaccaca tctacaggct ggtgaaagaa taagaattcg ggcccgttta aacccgctga
     3241 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     3301 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     3361 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     3421 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     3481 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     3541 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     3601 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     3661 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     3721 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     3781 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     3841 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     3901 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     3961 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4021 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     4081 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     4141 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     4201 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     4261 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     4321 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     4381 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     4441 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     4501 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     4561 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     4621 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     4681 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     4741 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     4801 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     4861 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     4921 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     4981 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     5041 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     5101 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     5161 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     5221 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     5281 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     5341 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     5401 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     5461 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     5521 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     5581 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     5641 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     5701 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     5761 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     5821 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     5881 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     5941 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6001 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6061 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     6121 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     6181 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     6241 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     6301 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     6361 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     6421 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     6481 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     6541 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     6601 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     6661 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     6721 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     6781 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     6841 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     6901 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     6961 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     7021 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     7081 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     7141 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     7201 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     7261 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     7321 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttac
//
