LOCUS       sequence-437151-        9208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of YAP/SPARK-ON (SFFV 
            promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9208)
  AUTHORS   Chung CI, Yang J, Shu X
  TITLE     Chemogenetic Minitool for Dissecting the Roles of Protein Phase 
            Separation.
  JOURNAL   ACS Cent Sci. 2023 Jul 7;9(7):1466-1479. doi: 
            10.1021/acscentsci.3c00251. eCollection 2023 Jul 26.
  PUBMED    37521779
REFERENCE   2  (bases 1 to 9208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acscentsci.3c00251"; journalName: "ACS Cent Sci"; date: 
            "2023-07-7- 7"; volume: "9"; issue: "7"; pages: "1466-1479"
COMMENT     Sequence Label: ZIF-NLS-EGFP*-HOTag6-T2A-CEL-EGFP-YAP
FEATURES             Location/Qualifiers
     source          1..9208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1189..1206)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1195..1419
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1465..1893
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1552..1571)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1762..1783
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1902..1922)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1907..2236
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2087..2222
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2149..2168
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2303..3097
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2357..2376)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2967..2986
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3271..3392
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3308..3327)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3362..3381
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3441..3457)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3441..3457)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3454..3476)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3465..3481
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3489..3495,3496..3513,3514..3519))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3534..3555
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3672..3689)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3843..4431)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3923..3942)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4602..5393,5394..5462))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5225..5244
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5463..5567)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5642..5661)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5833..6212
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6213..6416
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6367..6387
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6461..6480
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6461..6479
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6643..6669
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             7597..7650
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(8035..8037,8038..8040,8041..8751)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8080..8101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8341..8360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8688..8709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cacagcatgt tcgagctcat tcctctccag cttctctgca gttgggagct gtttctcctg
       61 ggacactgac ccccactgga gtagtctctg gcccagcagc tacacccaca gctcagcatc
      121 ttcgacagtc ttcttttgag atacctgatg atgtacctct gccagcaggt tgggagatgg
      181 caaagacatc ttctggtcag agatacttct taaatcacat cgatcagaca acaacatggc
      241 aggaccccag gaaggccatg ctgtcccaga tgaacgtcac agcccccacc agtccaccag
      301 tgcagcagaa tatgatgaac tcggcttcag gtcctcttcc tgatggatgg gaacaagcca
      361 tgactcagga tggagaaatt tactatataa accataagaa caagaccacc tcttggctag
      421 acccaaggct tgaccctcgt tttgccatga accagagaat cagtcagagt gctccagtga
      481 aacagccacc acccctggct ccccagagcc cacagggagg cgtcatgggt ggcagcaact
      541 ccaaccagca gcaacagatg cgactgcagc aactgcagat ggagaaggag aggctgcggc
      601 tgaaacagca agaactgctt cggcaggcaa tgcggaatat caatcccagc acagcaaatt
      661 ctccaaaatg tcaggagtta gccctgcgta gccagttacc aacactggag caggatggtg
      721 ggactcaaaa tccagtgtct tctcccggga tgtctcagga attgagaaca atgacgacca
      781 atagctcaga tcctttcctt aacagtggca cctatcactc tcgagatgag agtacagaca
      841 gtggactaag catgagcagc tacagtgtcc ctcgaacccc agatgacttc ctgaacagtg
      901 tggatgagat ggatacaggt gatactatca accaaagcac cctgccctca cagcagaacc
      961 gtttcccaga ctaccttgaa gccattcctg ggacaaatgt ggaccttgga acactggaag
     1021 gagatggaat gaacatagaa ggagaggagc tgatgccaag tctgcaggaa gctttgagtt
     1081 ctgacatcct taatgacatg gagtctgttt tggctgccac caagctagat aaagaaagct
     1141 ttcttacatg gttataagaa ttcgggcccg tttaaacccg ctgatcagcc tcgactgtgc
     1201 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     1261 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     1321 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     1381 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     1441 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     1501 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     1561 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     1621 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     1681 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     1741 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     1801 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     1861 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     1921 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     1981 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     2041 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     2101 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     2161 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     2221 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga
     2281 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     2341 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     2401 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     2461 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     2521 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     2581 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     2641 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     2701 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     2761 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     2821 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     2881 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     2941 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     3001 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     3061 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     3121 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     3181 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     3241 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     3301 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     3361 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     3421 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     3481 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     3541 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     3601 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     3661 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     3721 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     3781 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     3841 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     3901 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     3961 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     4021 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     4081 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     4141 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4201 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4261 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     4321 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     4381 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4441 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4501 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     4561 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     4621 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     4681 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     4741 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     4801 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     4861 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     4921 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     4981 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     5041 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     5101 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     5161 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     5221 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     5281 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     5341 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     5401 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     5461 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     5521 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     5581 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca
     5641 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
     5701 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
     5761 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
     5821 gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     5881 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     5941 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     6001 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     6061 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     6121 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     6181 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     6241 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     6301 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     6361 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct
     6421 ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact cactataggg
     6481 agacccaagc tggctagcgc caccatgact ggagaacggc ccttccagtg caatcagtgc
     6541 ggggcctcat tcacccagaa gggcaacctg ctccggcaca tcaagctgca ttccggggag
     6601 ccgcggtctg gtctgcgttc tggttctggt ggtggtatcg atccagcagc taaaagagtc
     6661 aaattggata tggtttctaa aggtgaagaa ctgtttactg gtgttgtacc gatcctggtt
     6721 gaactggacg gcgacgtaaa tggtcataaa ttctccgttt ctggtgaggg cgagggtgat
     6781 gcaacttatg gcaagctgac cctgaaattc atttgcacca ccggaaaact gccggttccg
     6841 tggccgaccc tggtgaccac tctgaccttt ggcgtacagt gcttctctcg ctacccggat
     6901 cacatgaaac agcacgactt tttcaaatct gcgatgccgg aaggttacgt acaagagcgt
     6961 accatcttct tcaaagatga cggtaattac aaaacaagag cggaagttaa attcgagggt
     7021 gacaccctgg tgaaccgcat cgaactgaaa ggcatcgact tcaaagagga tggcaacatc
     7081 ctgggccaca aactggaata caactacaac tcccacaacg tgtacatcat ggctgataag
     7141 cagaagaacg gcatcaaagc gaacttcaaa attcgtcaca acatcgaaga cggctctgtt
     7201 caactggcgg atcactacca gcagaacacg ccgattggcg acggcccggt tctgctgccg
     7261 gataaccact atctgtccac ccagtctgcc ctgtctaaag acccgaacga gaaacgtgac
     7321 catatggtac tgctggaatt tgttaccgcc gctggtatca ccctgggtat ggacgaactg
     7381 tacaagaccg gtggctccgc aggtggttcc gctggtggaa gcgccggtgg ttctgcgggt
     7441 ggatctgctg gaggttctgc aggaggaagc gctggaggtg ggcgcgccac cttgagagaa
     7501 atcgaggaat tgttgaggaa aatcattgaa gacagcgtgc gcagtgtggc cgagctggag
     7561 gacattgaga agtggctgaa gaaaatcggc ggccgcgagg gcagaggaag tctgctaaca
     7621 tgcggtgacg tcgaggagaa tcctggccca aagcttgcca ccatgtccct ttgctgtaaa
     7681 caatgtcaag aaacagaaat aacaaccaaa aatgaaatat tcagtttatc cttatgtggg
     7741 ccgatggcag cttatgtgaa tcctcatgga tatgtgcatg agacacttac tgtgtataag
     7801 gcttgcaact tgaatctgat aggccggcct tctacagaac acagctggtt tcctgggtat
     7861 gcctggactg ttgcccagtg taagatctgt gcaagccata ttggatggaa gtttacggcc
     7921 accaaaaaag acatgtcacc tcaaaaattt tggggcttaa cgcgatctgc tctgttgccc
     7981 acgatcccag acctcgagtc cggactcaga tctggcagcg gtggaggcga tatcatggtg
     8041 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     8101 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     8161 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     8221 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     8281 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     8341 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     8401 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     8461 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     8521 aaggccaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     8581 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     8641 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     8701 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggatccggc
     8761 agtgctggtg gtagtgctgg tggtagtgct ggtggtagtg ctggtggcag tgctggtggt
     8821 agtgctggtg gtagtgctgg tggctctaga atggatcccg ggcagcagcc gccgcctcaa
     8881 ccggcccccc agggccaagg gcagccgcct tcgcagcccc cgcaggggca gggcccgccg
     8941 tccggacccg ggcaaccggc acccgcggcg acccaggcgg cgccgcaggc accccccgcc
     9001 gggcatcaga tcgtgcacgt ccgcggggac tcggagaccg acctggaggc gctcttcaac
     9061 gccgtcatga accccaagac ggccaacgtg ccccagaccg tgcccatgag gctccggaag
     9121 ctgcccgact ccttcttcaa gccgccggag cccaaatccc actcccgaca ggccagtact
     9181 gatgcaggca ctgcaggagc cctgactc
//
