LOCUS       sequence-437152-        6999 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6999)
  TITLE     Human Transporter Knockout library
REFERENCE   2  (bases 1 to 6999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pENTR-ABCC1_S769N_STOP
FEATURES             Location/Qualifiers
     source          1..6999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     47..69
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     61..78
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     62..78
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    131..230
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      231..240
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(4865..4964)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5021..5037)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5021..5037)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5034..5056)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(5209..5797)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5289..5308)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5933..6748)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5933..6748)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6656..6675
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6740..6759
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     6869..6885
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6869..6885
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      6886..6932
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aaggaagtct ccaataactg tgatccacca caagcgccag ggttttccca gtcacgacgt
       61 tgtaaaacga cggccagtca tgcataatcc gcacgcatct ggaataagga agtgccattc
      121 cgcctgacct caaataatga ttttattttg actgatagtg acctgttcgt tgcaacagat
      181 tgatgagcaa tgctttttta taatgccaac tttgtacaaa aaagcaggct gccaccatgg
      241 cactccgagg cttttgtagc gcagatggca gcgaccccct gtgggattgg aatgtaacat
      301 ggaatacaag caaccctgac ttcacaaaat gttttcaaaa tacagtactc gtctgggtcc
      361 cctgcttcta cttgtgggca tgtttcccct tctacttctt gtatctctcc aggcacgata
      421 ggggatacat ccaaatgacc cctctgaata aaaccaaaac tgctctgggc ttcctgttat
      481 ggatagtctg ctgggccgac ttattttatt ccttttggga gcgctcaagg ggcatcttcc
      541 tcgcccccgt tttccttgta tctcctacgt tgcttggaat cactatgtta ttagctactt
      601 tcctgatcca actcgaaaga agaaaagggg ttcaaagcag tggaataatg ctcacctttt
      661 ggctcgtagc cttggtgtgc gcactcgcaa tcttgaggtc aaagatcatg actgcactga
      721 aggaagatgc ccaagtagat ttgtttcgcg acataacttt ctacgtgtat ttctcacttc
      781 ttctgatcca actggtcctt tcttgcttct ccgatcggag ccctttattt tccgagacca
      841 tccatgaccc aaacccttgt ccagagagct ctgcgagttt cttatctagg atcaccttct
      901 ggtggatcac cggtttaatc gttcgcggtt atcggcagcc gcttgagggc tctgaccttt
      961 ggagtcttaa caaagaggat acgtcagaac aagtggttcc tgtactggtg aagaattgga
     1021 agaaagaatg tgccaaaact cgcaagcaac ccgttaaagt agtgtattct agcaaggatc
     1081 ccgcccagcc gaaagagtca tccaaagtgg atgccaatga ggaagttgaa gcattgattg
     1141 taaagtctcc tcagaaagag tggaaccctt cattgtttaa ggttctgtat aagacatttg
     1201 gtccctactt tctgatgtct ttcttcttca aagcgatcca tgaccttatg atgttttcag
     1261 gtccccaaat tctgaagtta cttattaagt ttgtgaatga taccaaggca ccggattggc
     1321 aggggtattt ctacactgtt ctcctgtttg taacagcgtg ccttcaaaca ctcgtcttac
     1381 accagtattt ccacatatgc ttcgtgtcag gaatgaggat taagacagcc gttatcggtg
     1441 ctgtctacag aaaggctctc gtcatcacta acagcgcgag aaaatcttcc acagtgggtg
     1501 aaattgtgaa cctgatgagc gtggatgcgc aaagatttat ggacctcgcc acctatatta
     1561 acatgatatg gagcgcaccc ctgcaggtca tattggcact ctacttgctt tggttgaacc
     1621 tgggtccaag tgttctggct ggtgttgctg tgatggtgtt gatggtaccc gttaacgccg
     1681 tcatggccat gaagactaag acatatcaag tcgctcatat gaaatccaag gacaaccgaa
     1741 taaaactcat gaacgaaatc ctgaatggta ttaaagtgct taaactgtat gcttgggaac
     1801 tggctttcaa agataaggtc ttggctatcc gtcaagaaga attgaaagtt cttaagaaat
     1861 cagcttacct ctcagccgtc ggcacgttca cctgggtctg cacacccttt ctggtggcct
     1921 tgtgtacttt cgccgtatat gtcactattg acgagaacaa tatcctggac gcacaaaccg
     1981 cctttgtatc cctcgcgctg tttaacattc tccggtttcc gcttaatatc ttgcctatgg
     2041 tgatctcaag tattgtccaa gccagtgtct ctctgaaacg gctccgcatc tttctgtctc
     2101 atgaggagtt ggaaccagat tctatagaac gccgacccgt gaaagacggc ggcgggacca
     2161 acagcataac tgtcagaaac gcaacattca catgggctcg cagtgacccg cccaccctca
     2221 atggcattac attctccatt ccggaaggcg cattagtcgc agtggtgggg caagttggat
     2281 gtggcaagtc cagcctgctc tccgctctgc ttgccgagat ggacaaagtt gagggacatg
     2341 tcgctataaa agggtctgtc gcatatgtcc cccaacaagc ctggatacag aatgattctt
     2401 tacgagagaa catattgttt ggatgtcaac tcgaagaacc ctattaccgg tccgttattc
     2461 aagcatgtgc tctcttgcct gacctcgaga ttcttcccag cggcgaccgt acagagatcg
     2521 gcgagaaagg cgtcaatctc aatggtggcc aaaagcagcg cgtcagcttg gcacgtgccg
     2581 tgtattctaa tgctgatatt tatctgttcg atgatccact ctctgccgtg gatgcccatg
     2641 tggggaaaca catattcgag aatgtaatag ggcccaaggg tatgcttaag aataaaacta
     2701 ggattctggt aacccatagt atgtcttatt tgccacaagt tgatgtcatt atcgtcatgt
     2761 ctggcggaaa gattagtgag atgggctctt accaagaact gctggcccgg gacggggcct
     2821 ttgccgaatt cctgcgtact tacgcctcaa cggaacagga gcaggatgct gaggagaatg
     2881 gagtaaccgg agttagcggg ccaggtaaag aggctaaaca aatggagaac gggatgttag
     2941 tcactgactc tgcaggaaaa caactgcaac gccagctgag cagcagcagt agctattccg
     3001 gcgacatttc ccgacaccac aactctactg cggaacttca gaaagcggaa gctaagaaag
     3061 aggaaacttg gaaactgatg gaagctgata aggctcaaac cgggcaagtg aaactgtctg
     3121 tttattggga ttatatgaag gccattgggt tgtttattag cttcctgtca attttcctgt
     3181 tcatgtgtaa tcatgtttca gctttagcct ctaactattg gctttctctc tggaccgatg
     3241 atcctattgt taatggtact caagagcata ctaaagtaag gctcagcgtg tatggtgccc
     3301 tgggcataag ccaaggtatc gccgtatttg ggtacagcat ggctgtatcc atcggtggaa
     3361 tcctcgcgtc acgctgcctt catgtcgact tgttacattc aatactgagg agtcctatgt
     3421 ccttctttga aagaacacct agcggaaacc tggtcaaccg attttcaaag gaactggata
     3481 ctgtcgactc catgattcct gaggttatta aaatgtttat gggctcttta tttaacgtga
     3541 ttggagcctg tatcgtcatc ctgcttgcca cgccaatcgc agcgataatc attccacccc
     3601 tgggacttat ctacttcttt gtgcaacgct tctacgtggc gtcatcccga cagctcaaac
     3661 gcctggaaag cgtctccaga agcccagtgt actcacattt caacgagacc ctgcttgggg
     3721 tttccgtgat aagggctttc gaggaacagg aacgctttat acatcaatcc gacttgaaag
     3781 tggatgagaa ccaaaaggcc tattatccgt ccatagtagc aaacaggtgg ctggctgtac
     3841 gtctggagtg cgtgggaaat tgcattgttc tgtttgcagc tttatttgcc gttatatcca
     3901 ggcacagtct ctcagccggg ctcgttgggt tgtctgtcag ctactcactg caagtgacga
     3961 cttatcttaa ttggcttgtg cgcatgagct cagaaatgga aacaaatatc gtagcagttg
     4021 aacggcttaa agaatactca gaaacagaga aagaggcacc ttggcaaata caagaaactg
     4081 caccaccttc cagttggcct caagtcggcc gggtcgagtt cagaaattat tgtctgaggt
     4141 atcgagaaga cctggacttt gtgctgagac atattaatgt taccattaat ggcggtgaaa
     4201 aggtcgggat tgtaggaagg actggtgcag gtaaatccag ccttacgtta ggccttttcc
     4261 gtatcaacga atccgctgaa ggcgagatca taatagatgg gattaatata gccaaaatcg
     4321 gccttcatga tcttcgcttc aagatcacta tcatccctca ggaccccgtt ttgttttctg
     4381 gaagcttacg catgaacttg gatccattct cacagtattc cgacgaagaa gtgtggactt
     4441 cactcgaatt ggcgcacctg aaggactttg tgagcgcttt gccagacaag ctcgaccatg
     4501 aatgtgccga aggcggtgag aacttaagcg taggccaacg tcagttagtt tgccttgctc
     4561 gggcgctgct ccgaaagact aagattctcg tattagatga agccactgcg gcagttgacc
     4621 ttgaaacaga tgacctcata cagagcacga tcagaacgca atttgaagac tgtacagttc
     4681 tcaccatcgc gcatagactt aacaccatta tggattacac aagagtcatt gtgttagaca
     4741 agggtgagat ccaagagtac ggggccccaa gtgatctgtt acaacaacga gggctgttct
     4801 actctatggc aaaggacgcc ggattagtat gacttgtgga aaggacgtgt cacttgaacg
     4861 cgggacccag ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt
     4921 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgaggcta ggtggaggct
     4981 cagtgatgat aagtctgcga tggtggatgc atgtgtcatg gtcatagctg tttcctgtgt
     5041 gaaattgtta tccgctcaga gggcacaatc ctattccgcg ctatccgaca atctccaaga
     5101 cattaggtgg agttcagttc ggcgtatggc atatgtcgct ggaaagaaca tgtgagcaaa
     5161 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     5221 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     5281 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     5341 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     5401 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     5461 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     5521 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     5581 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     5641 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     5701 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     5761 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     5821 ggggtctgac gctctattca acaaagccgc cgtcccgtca agtcagcgta aatgggtagg
     5881 gggcttcaaa tcgtccgctc tgccagtgtt acaaccaatt aacaaattct gattagaaaa
     5941 actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt
     6001 tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg
     6061 caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt
     6121 tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg
     6181 gtgagaatgg caaaagctta tgcatttctt tccagacttg ttcaacaggc cagccattac
     6241 gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag
     6301 cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc
     6361 ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta
     6421 atacctggaa tgctgttttc ccggggatcg cagtggtgag taaccatgca tcatcaggag
     6481 tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga
     6541 ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg
     6601 gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc
     6661 gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctcgagc
     6721 aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag
     6781 acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt
     6841 gagacacaac gtggctttcc cccgccgctc tagaactagt ggatccaaat aaaacgaaag
     6901 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcgcatta tacgagacgt
     6961 ccaggttggg atacctgaaa caaaacccat cgtacggcc
//
