LOCUS       sequence-437154-        3769 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pENTR-MPP1
FEATURES             Location/Qualifiers
     source          1..3769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1505..1604)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1661..1677)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1661..1677)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1674..1696)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1849..2437)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1929..1948)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2573..3388)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2573..3388)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3296..3315
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3380..3399
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     3509..3525
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3509..3525
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      3526..3572
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3686..3708
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3700..3717
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3701..3717
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacagat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgac gctcaaggcc
      121 tctgaaggag aaagtggcgg ttcaatgcac acggcgctgt ctgacctcta cttggagcac
      181 ctgctccaaa aacgctctag acctgaggca gtgagtcacc ctctgaatac cgtgacggag
      241 gacatgtaca ccaacggctc tccagcccca ggatctcctg cacaggtcaa gggtcaggag
      301 gtgaggaaag tacgtttaat acagttcgag aaagtgacag aggaaccaat gggcattacc
      361 ctgaagctga atgagaagca gtcctgtaca gtggcaagaa ttcttcacgg tggcatgata
      421 catcggcagg ggtccctgca cgtcggggac gagatactgg agattaatgg caccaacgta
      481 actaaccata gcgtggacca gctgcagaag gccatgaaag aaactaaagg gatgatcagt
      541 ctgaaagtga tcccaaacca gcagtctcga ctgcccgcac tgcaaatgtt tatgcgtgcc
      601 cagttcgatt atgaccccaa gaaggataat cttatcccat gcaaagaggc cgggctgaag
      661 tttgccactg gtgacatcat ccagattatc aataaggatg acagcaactg gtggcagggc
      721 agggtggaag gaagctctaa agaatctgct ggtcttatcc cctcccctga gctgcaagag
      781 tggcgagtcg catctatggc acaaagcgct cccagtgaag cccccagctg ctcacctttc
      841 ggaaaaaaga aaaagtataa ggataaatac cttgccaagc actccagcat cttcgatcag
      901 ttggatgttg tcagttacga agaagttgtc aggctgcccg ccttcaaaag aaaaacactg
      961 gtgctgatcg gggccagtgg agttggaaga agtcacatta agaacgcatt gttatctcaa
     1021 aatccggaga agttcgtgta tcctgttcca tatactacac ggccacctcg taagtccgag
     1081 gaagatggca aagagtatca tttcatctct actgaggaaa tgaccagaaa catcagcgca
     1141 aacgagttcc ttgaattcgg atcatatcag ggtaatatgt tcggaacaaa gttcgagact
     1201 gtgcaccaga ttcacaagca gaataaaatc gcgatcctcg acattgagcc ccagaccctc
     1261 aagatcgtgc ggactgccga actgtccccg tttatcgtgt ttatcgctcc cactgatcag
     1321 ggaacccaga ctgaagcctt acagcagctc caaaaggact ctgaagcgat ccggagccaa
     1381 tatgcgcact acttcgactt gtcattagta aataacgggg tagacgaaac gctgaagaag
     1441 cttcaggagg ctttcgacca agcctgctct agtccccaat gggtccctgt ttcctgggtt
     1501 tacgacccag ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt
     1561 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgaggcta ggtggaggct
     1621 cagtgatgat aagtctgcga tggtggatgc atgtgtcatg gtcatagctg tttcctgtgt
     1681 gaaattgtta tccgctcaga gggcacaatc ctattccgcg ctatccgaca atctccaaga
     1741 cattaggtgg agttcagttc ggcgtatggc atatgtcgct ggaaagaaca tgtgagcaaa
     1801 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     1861 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     1921 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     1981 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     2041 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     2101 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     2161 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     2221 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     2281 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     2341 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     2401 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     2461 ggggtctgac gctctattca acaaagccgc cgtcccgtca agtcagcgta aatgggtagg
     2521 gggcttcaaa tcgtccgctc tgccagtgtt acaaccaatt aacaaattct gattagaaaa
     2581 actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt
     2641 tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg
     2701 caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt
     2761 tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg
     2821 gtgagaatgg caaaagctta tgcatttctt tccagacttg ttcaacaggc cagccattac
     2881 gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag
     2941 cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc
     3001 ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta
     3061 atacctggaa tgctgttttc ccggggatcg cagtggtgag taaccatgca tcatcaggag
     3121 tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga
     3181 ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg
     3241 gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc
     3301 gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctcgagc
     3361 aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag
     3421 acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt
     3481 gagacacaac gtggctttcc cccgccgctc tagaactagt ggatccaaat aaaacgaaag
     3541 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcgcatta tacgagacgt
     3601 ccaggttggg atacctgaaa caaaacccat cgtacggcca aggaagtctc caataactgt
     3661 gatccaccac aagcgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtcat
     3721 gcataatccg cacgcatctg gaataaggaa gtgccattcc gcctgacct
//
