LOCUS       sequence-437156-        5149 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 5149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pENTR-CASK
FEATURES             Location/Qualifiers
     source          1..5149
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2885..2984)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3041..3057)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3041..3057)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3054..3076)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(3229..3817)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3309..3328)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3953..4768)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3953..4768)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4676..4695
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4760..4779
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     4889..4905
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4889..4905
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      4906..4952
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5066..5088
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5080..5097
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5081..5097
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacagat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc agacgatgac
      121 gtgttatttg aagacgtgta tgaactgtgt gaggttatcg ggaaaggccc tttttccgta
      181 gtccgaagat gcattaaccg agaaaccgga cagcaattcg cggtaaagat cgtggacgta
      241 gcaaagttca ctagtagtcc cgggctgagt accgaagact tgaagcgaga ggcttccatt
      301 tgccacatgc ttaagcaccc acatattgtg gagttactgg aaacctattc tagtgatggg
      361 atgctgtata tggtgttcga gttcatggat ggagcagacc tgtgtttcga gattgtcaag
      421 agggctgacg ccggctttgt gtacagtgaa gccgttgcaa gtcattacat gcgtcaaatc
      481 ttagaggcct tacggtactg ccacgacaat aacatcattc acagggacgt gaagcctcat
      541 tgcgtgcttc tggccagtaa agagaactca gccccagtca aactgggagg attcggagtc
      601 gcgattcagt tgggagagag cggcctcgtg gccggcggaa gggtcggtac accacacttt
      661 atggcccctg aagtagttaa gcgtgagccc tatgggaagc ctgttgatgt gtggggatgt
      721 ggggtcatcc tctttatact cctgtcaggc tgtctgccat tttacggcac caaggaacga
      781 ctcttcgaag gcatcattaa gggcaaatat aagatgaacc ctcggcagtg gtctcatatc
      841 tccgaatccg ctaaggatct cgttcgcaga atgctgatgc tggaccctgc ggagcggatt
      901 acggtttatg aggccctcaa ccacccctgg ttgaaagagc gggatcgcta cgcatataaa
      961 atccacctgc cagaaacagt ggaacagctc cgaaaattca atgcaaggag gaagctcaag
     1021 ggggccgtgc tcgctgcagt gagctcacac aagtttaaca gcttctacgg agaccctccg
     1081 gaagagcttc cagactttag tgaggaccct accagcagtg ggctcctggc agctgagcga
     1141 gctgtgtccc aggtccttga cagcttggag gagatccatg cccttactga ttgcagcgaa
     1201 aaggatttag actttctcca ttctgtgttt caggaccagc acctccatac tttattagat
     1261 ctgtatgaca aaattaacac taagagctct ccacagatta ggaatccacc cagcgacgct
     1321 gtgcaaagag ccaaggaagt gttagaagag atttcttgct atcctgagaa taatgacgcg
     1381 aaagagctga agcggatttt gacacaaccg cacttcatgg cccttttaca gacacatgac
     1441 gtagtagctc atgaggttta tagtgatgag gccctgcgcg taactccccc acccaccagc
     1501 ccttatctga atggagactc ccccgagtcc gccaatgggg acatggatat ggagaacgtt
     1561 actcgggtgc gcttagtgca gttccaaaag aacactgatg aacccatggg tatcaccctg
     1621 aaaatgaatg agcttaacca ttgcattgtt gcgcgcatca tgcatggagg catgatccat
     1681 cgtcagggaa ctttacatgt tggtgacgaa attagggaaa ttaacggaat tagcgtagct
     1741 aatcagaccg tggagcagct ccaaaagatg ctgagggaga tgcgcggatc cattacattt
     1801 aagatagtgc catcctaccg aacccagagt agttcatgtg aacgcgatag cccatccaca
     1861 tcaaggcagt ctcctgcgaa cggccatagc tccaccaaca attccgtgag cgaccttccc
     1921 tccacgacgc agcctaaagg aaggcagatt tacgtccgtg cacagtttga gtatgaccca
     1981 gcgaaggatg acttgatccc atgtaaagag gccggaatac gatttagggt aggggacatt
     2041 atacagatca tatccaagga tgaccacaac tggtggcagg ggaagctgga aaatagcaag
     2101 aatggcactg ctggccttat accgtctcca gaactgcagg agtggagggt cgcttgcatt
     2161 gcaatggaaa aaactaagca agaacaacag gcgtcctgta cttggttcgg caaaaagaaa
     2221 aagcagtata aagataaata tctcgccaag cacaatgctg tgttcgatca gctggacctc
     2281 gtgacctacg aggaggttgt gaagcttcct gcttttaagc ggaagacgct ggtgctcttg
     2341 ggagcgcatg gggtggggag gcgccacatc aagaatacgc tcattaccaa gcaccccgac
     2401 agattcgcat accctatccc ccacactacg aggccaccga aaaaggacga ggagaacggc
     2461 aaaaattact atttcgtgag tcatgaccag atgatgcagg acatctcaaa taatgaatat
     2521 cttgaatatg gctcacacga ggacgccatg tacggaacga agctggagac catcaggaag
     2581 atccacgaac agggattaat cgcgatcctg gatgtggaac cccaggccct caaggttctg
     2641 cgaactgccg aattcgcccc gtttgtggtg tttattgcag cccctaccat tacccccgga
     2701 cttaacgagg atgaatccct gcagcgctta cagaaggaat ctgacatcct ccagaggacc
     2761 tacgctcact actttgacct gacaattatt aacaacgaga tagacgaaac catcagacac
     2821 ctggaagaag cagtcgaact ggtgtgcacc gctccccagt gggttcctgt gagctgggtg
     2881 tatgacccag ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt
     2941 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgaggcta ggtggaggct
     3001 cagtgatgat aagtctgcga tggtggatgc atgtgtcatg gtcatagctg tttcctgtgt
     3061 gaaattgtta tccgctcaga gggcacaatc ctattccgcg ctatccgaca atctccaaga
     3121 cattaggtgg agttcagttc ggcgtatggc atatgtcgct ggaaagaaca tgtgagcaaa
     3181 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     3241 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     3301 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     3361 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     3421 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3481 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3541 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3601 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3661 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3721 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3781 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3841 ggggtctgac gctctattca acaaagccgc cgtcccgtca agtcagcgta aatgggtagg
     3901 gggcttcaaa tcgtccgctc tgccagtgtt acaaccaatt aacaaattct gattagaaaa
     3961 actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt
     4021 tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg
     4081 caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt
     4141 tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg
     4201 gtgagaatgg caaaagctta tgcatttctt tccagacttg ttcaacaggc cagccattac
     4261 gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag
     4321 cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc
     4381 ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta
     4441 atacctggaa tgctgttttc ccggggatcg cagtggtgag taaccatgca tcatcaggag
     4501 tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga
     4561 ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg
     4621 gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc
     4681 gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctcgagc
     4741 aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag
     4801 acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt
     4861 gagacacaac gtggctttcc cccgccgctc tagaactagt ggatccaaat aaaacgaaag
     4921 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcgcatta tacgagacgt
     4981 ccaggttggg atacctgaaa caaaacccat cgtacggcca aggaagtctc caataactgt
     5041 gatccaccac aagcgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtcat
     5101 gcataatccg cacgcatctg gaataaggaa gtgccattcc gcctgacct
//
