LOCUS       sequence-437159-       11175 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A piggyBAC based plasmid for the stable expression of BRD4 with a 
            flag tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11175)
  TITLE     piggyBAC-BRD4-Flag
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: piggyBAC-BRD4-Flag
FEATURES             Location/Qualifiers
     source          1..11175
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..63
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        516..727
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             740..1008
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             1071..1094
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3336..3353
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    join(5357..5931,5932..5934,5935..5943)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5524..5541)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5751..5770
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5956..6750
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLAATGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6010..6029)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6620..6639
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6810..6830)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    6872..6993
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6909..6928)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6963..6982
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7756..7790
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8281..8297)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8281..8297)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8294..8316)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8305..8321
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8329..8335,8336..8353,8354..8359))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8374..8395
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8512..8529)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8683..9271)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8763..8782)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9442..10233,10234..10302))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10065..10084
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10303..10407)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(10433..10888)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10570..10591)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10782..10801
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11015..11037
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11029..11046
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11030..11046
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ccctagaaag ataatcatat tgtgacgtac gttaaagata atcatgtgta aaattgacgc
       61 atgtgtttta tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt
      121 tattatattt acacttacat actaataata aattcaacaa acaatttatt tatgtttatt
      181 tatttattaa aaaaaacaaa aactcaaaat ttcttctata aagtaacaaa acttttatga
      241 gggacagccc ccccccaaag cccccaggga tgtaattacg tccctccccc gctagggggc
      301 agcagcgagc cgcccggggc tccgctccgg tccggcgctc cccccgcatc cccgagccgg
      361 cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg ggatcgcttt cctctgaacg
      421 cttctcgctg ctctttgagc ctgcagacac ctggggggat acggggaaaa ggcctccacg
      481 gccaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
      541 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg
      601 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
      661 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
      721 aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg
      781 aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg
      841 aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc
      901 gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc
      961 tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc
     1021 ggcgcctact ctagagctag cgcaccatgg attacaagga tcatgacatt gactacaaag
     1081 acgatgacga caagggtggt tctggtggtt ctggtggttc tatgtctacg gagagcggcc
     1141 ctgggacaag attgagaaat ctgccagtaa tgggggatgg actagaaacc tcccaaatgt
     1201 ctacaacgca ggcccaggcc caaccccagc cagcaaatgc agccagcacc aatcctccac
     1261 ccccagagac ctccaaccct aacaagccca agagacagac aaaccaactg caatatctgc
     1321 tcagagtggt gctcaagaca ctatggaaac accagtttgc gtggcctttc cagcagcccg
     1381 tggatgccgt caagctgaac ctccctgatt actataagat tattaaaaca cccatggata
     1441 tgggaacaat aaagaagcgc ttggaaaaca actattactg gaatgctcag gaatgtatcc
     1501 aggacttcaa cactatgttt acaaattgtt acatctataa caagcctgga gatgacatcg
     1561 tcttaatggc agaagctctg gagaagctct tcttgcaaaa aatcaatgaa ctgcctacag
     1621 aagaaactga gatcatgata gtccaggcaa aaggaagagg acgagggagg aaagaaacag
     1681 gggcagcaaa gcctggtgta tccacggtac caaacacaac tcaagcatca acttctccgc
     1741 agacccagac gcctcagcag aaccctcctc cacctgtgca ggccacaact cacccctttc
     1801 ctgctgtcac cccagacctc attgcccagc ctcctgtcat gacaatggtg ccccctcagc
     1861 cacttcagac tccttcaccg gtaccccccc agccaccacc cccacctgct ccagttccac
     1921 agcctgtgca gagtcacccg cccatcattg cgaccacccc ccagcctgtg aagacaaaga
     1981 aaggggtgaa gaggaaagca gataccacca cccctaccac catcgacccc attcatgagc
     2041 caccctcact ggccccagag cccaagaccg ccaagctggg tcctcggcgg gagagcagca
     2101 gacctgtgaa gcctccaaag aaggatgtac cggactcaca gcagcaccca gggccagaga
     2161 agagcagcaa gatctctgag cagctaaagt gctgcagtgg catcctcaag gagatgtttg
     2221 ccaagaaaca tgctgcctat gcctggcctt tctacaagcc tgtggatgtg gaggcactgg
     2281 gtctgcacga ctactgtgac atcatcaaac atcccatgga catgagcaca atcaagtcta
     2341 aactagagtc ccgagagtac agagatgccc aggaatttgg tgctgatgtc cgattgatgt
     2401 tctccaactg ctacaagtac aacccccctg accatgaagt ggtagccatg gctcgaaaac
     2461 tccaggatgt gtttgaaatg cgctttgcca agatgcctga tgagcctgaa gagccagttg
     2521 ttacagtgtc ctctcctgca gtgccacccc ctacaaaggt ggtagcccca ccctcatcta
     2581 gtgacagcag cagcgacagt tcttccgaca gcgacagttc cactgacgac tctgaggaag
     2641 agcgagccca gcggctggct gaactccagg aacagctcaa ggccgtgcat gagcagcttg
     2701 cagccctctc acagccccag cagaacaaac caaagaaaaa ggagaaggac aagaaggaaa
     2761 agaaaaagga aaagcacaaa aagaaagaag aagtggagga aaataaaaaa agcaaaacca
     2821 aggaacttcc tcccaaaaag acaaagaaaa ataacagcag caacagcaat gtgagcaaga
     2881 aggaaccagt acccacgaag accaagccgc ctcccacata tgaatcagaa gaggaggata
     2941 agtgtaagcc catgtcttat gaggagaagc ggcagctaag tctagatatc aacaaacttc
     3001 ctggtgagaa gctaggccgt gtagtacaca taattcagtc aagggaacca tcacttaaaa
     3061 actccaaccc cgatgagatt gagattgact ttgagaccct gaagccatct acactacgag
     3121 agttggagcg atatgtcacc tcctgtttgc ggaagaaaag gaaacctcaa gctgaaaaag
     3181 ttgacgtgat tgctggttct tccaagatga agggattctc atcctctgag tcggagagca
     3241 ccagcgaatc cagctcctct gacagtgaag actctgaaac agagatggct cccaagtcaa
     3301 aaaagaaggg gcacactggg agggaccaga aaaagcatca tcaccatcac catccacaga
     3361 tgcagccagc cccagctcct gtgccccagc agccgccccc acctccacag cagcctcctc
     3421 cacccccacc tccgcagcag cagcagcagc aacctccacc cccaccacct ccgccctcca
     3481 tgccacagca gactgcccca gcgatgaagt cctcgccccc acccttcatc actgcccagg
     3541 tccccgtcct ggaaccacag ctgccaggca gtgtctttga ccctattggc cacttcaccc
     3601 agcccatctt gcacctgccg cagccggagc tgcctcctca cctgccccag ccacctgagc
     3661 acagcactcc accccatctc aaccagcatg ctgtggtctc tcctccagct ttgcacaatg
     3721 cgctgcccca acagccatct cggcccagta accgagctgc tgctctgccc ccaaagccta
     3781 cccgaccccc agctgtgtcc cctgccctgg cccagccccc cctgctccca caaccaccaa
     3841 tggctcagcc cccccaagtg ctgctggagg atgaagagcc acctgcccca cccctcacct
     3901 ccatgcagat gcagttgtac ctacagcagc tgcagaaggt gcagcctccc acaccactac
     3961 tcccttccgt gaaggtgcag tcccaacccc caccgccttt gccgcctcca ccccaccctt
     4021 ctgtgcagca gcagcagctc cagccacagc caccgccacc cccacctcct cagccacagc
     4081 caccacccca gcaacaacac cagcctccac cacgaccagt tcacttgcca tccatgccct
     4141 tttctgctca tattcagcag cccccaccac ccccaggaca gcaacctact cacccacccc
     4201 caggacagca gcccccacca ccacagcctg ccaagcccca gcaagtcatc cagcatcacc
     4261 cttccccccg gcaccacaag tcagacccct actcagctgg tcatcttcgt gaggctccct
     4321 ctccgcttat gatacattcc cctcagatgc cacagttcca gagcctgacc catcagtctc
     4381 ctccccagca aaacgtccag cctaagaagc aggtaaaggg cagggctgag ccacagccac
     4441 cagggccagt catgggccaa ggccagggat gcccacccgc ctcaccggct gccgtgccta
     4501 tgctgtccca ggagctgcga ccaccttcag tcgtccagcc ccagcccctg gtggtagtaa
     4561 aggaggagaa gattcactca ccaatcattc gcagcgagcc tttcagcacc tcacttcgac
     4621 cagagccccc caagcaccca gagaacatca aggccccagt ccacctgccc cagcggcctg
     4681 agatgaagcc tgtagacata gggaggcctg tgatccggcc tccagagcag agtgcacccc
     4741 caccaggggc ccctgacaag gacaaacaga agcaggagcc aaaaacacca gtggcaccca
     4801 aaaaggacct gaaaattaag aacatgggct cctgggccag cctggtacag aagcatccga
     4861 ccaccccatc ctccacagcc aagtcctcaa gtgacagctt tgagcatttc cgccgtgctg
     4921 ctcgggagaa ggaggagagg gaaaaggccc tgaaggctca ggctgagcat gcagagaagg
     4981 agaaggagcg gctgaggcag gagcgcatga ggagccgaga ggatgaagat gcgctggaac
     5041 aggcacgtcg agcccatgag gaggcacgac ggcgccagga gcagcagcag cagcagcagc
     5101 agcagagaca ggagcagcag cagcagcagc agcaggcagc tgctgtggcc gctgcctctg
     5161 ccccccaggc ccagagctcc cagccccagt ctatgctgga ccagcagagg gagttggccc
     5221 ggaaacgaga gcaggagcgg aggcgcaggg aggcaatggc agctacaatt gacatgaatt
     5281 tccagagtga tcttttgtca atatttgaag aaaatctttt ttgagaattc gaatttaaat
     5341 cggatccgcg gccgcgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct
     5401 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     5461 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     5521 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     5581 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     5641 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     5701 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     5761 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     5821 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
     5881 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca
     5941 accatggcgt ccggaatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     6001 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     6061 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     6121 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccgc gacgggcgtt
     6181 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     6241 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     6301 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     6361 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     6421 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     6481 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     6541 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     6601 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     6661 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     6721 ttctatcgcc ttcttgacga gttcttctga gtcgacaatc aacctctgga ttacaaaatt
     6781 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     6841 gctttaatgc ctttgtatca tgcgttaact aaacttgttt attgcagctt ataatggtta
     6901 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     6961 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggaattgac tcaaatgatg
     7021 tcaattagtc tatcagaagc tcatctggtc tcccttccgg gggacaagac atccctgttt
     7081 aatatttaaa cagcagtgtt cccaaactgg gttcttatat cccttgctct ggtcaaccag
     7141 gttgcagggt ttcctgtcct cacaggaacg aagtccctaa agaaacagtg gcagccaggt
     7201 ttagccccgg aattgactgg attccttttt tagggcccat tggtatggct ttttccccgt
     7261 atccccccag gtgtctgcag gctcaaagag cagcgagaag cgttcagagg aaagcgatcc
     7321 cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg ccggctcggg gatgcggggg
     7381 gagcgccgga ccggagcgga gccccgggcg gctcgctgct gccccctagc gggggaggga
     7441 cgtaattaca tccctggggg ctttgggggg gggctgtccc tgatatctat aacaagaaaa
     7501 tatatatata ataagttatc acgtaagtag aacatgaaat aacaatataa ttatcgtatg
     7561 agttaaatct taaaagtcac gtaaaagata atcatgcgtc attttgactc acgcggtcgt
     7621 tatagttcaa aatcagtgac acttaccgca ttgacaagca cgcctcacgg gagctccaag
     7681 cggcgactga gatgtcctaa atgcacagcg acggattcgc gctatttaga aagagagagc
     7741 aatatttcaa gaatgcatgc gtcaatttta cgcagactat ctttctaggg ttaatctagc
     7801 tgcatcagga tcatatcgtc gggtcttttt tccggctcag tcatcgccca agctggcgct
     7861 atctgggcat cggggaggaa gaagcccgtg ccttttcccg cgaggttgaa gcggcatgga
     7921 aagagtttgc cgaggatgac tgctgctgca ttgacgttga gcgaaaacgc acgtttacca
     7981 tgatgattcg ggaaggtgtg gccatgcacg cctttaacgg tgaactgttc gttcaggcca
     8041 cctgggatac cagttcgtcg cggcttttcc ggacacagtt ccggatggtc agcccgaagc
     8101 gcatcagcaa cccgaacaat accggcgaca gccggaactg ccgtgccggt gtgcagatta
     8161 atgacagcgg tgcggcgctg ggatattacg tcagcgagga cgggtatcct ggctggatgc
     8221 cgcagaaatg gacatggata ccccgtgagt tacccggcgg gcgcgcttgg cgtaatcatg
     8281 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     8341 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     8401 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     8461 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     8521 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     8581 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8641 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8701 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8761 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8821 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8881 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8941 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9001 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     9061 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     9121 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     9181 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     9241 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     9301 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     9361 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     9421 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     9481 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9541 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     9601 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     9661 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9721 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     9781 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9841 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     9901 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     9961 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10021 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10081 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    10141 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    10201 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    10261 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    10321 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    10381 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
    10441 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
    10501 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
    10561 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
    10621 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
    10681 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
    10741 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
    10801 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
    10861 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg
    10921 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
    10981 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
    11041 gccagtgagc gcgcctcgtt cattcacgtt tttgaacccg tggaggacgg gcagactcgc
    11101 ggtgcaaatg tgttttacag cgtgatggag cagatgaaga tgctcgacac gctgcagaac
    11161 acgcagctag attaa
//
