LOCUS       sequence-437160-        3890 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cdGreen expressed from an aTc-inducible promoter (Internal Lab ID: 
            UJ11204).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Kaczmarczyk A, van Vliet S, Jakob RP, Teixeira RD, Scheidat I, 
            Reinders A, Klotz A, Maier T, Jenal U
  TITLE     A genetically encoded biosensor to monitor dynamic changes of 
            c-di-GMP with high temporal resolution.
  JOURNAL   Nat Commun. 2024 May 9;15(1):3920. doi: 10.1038/s41467-024-48295-0.
  PUBMED    38724508
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-05-9"; volume: "15(1):3920. doi"; pages: " 
            10.1038/s41467-024-48295-0"
COMMENT     Sequence Label: p2H12
FEATURES             Location/Qualifiers
     source          1..3890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(25..43,44..49,50..66,67..72,73..98)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    25..43
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    50..68
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             124..747
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(197..216)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     1278..1299
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1405..1426)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        2111..2215
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2216..2284,2285..3076)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2434..2453)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3247..3835
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3736..3755
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acatgttttc gtcttcacct cgagtcccta tcagtgatag agattgacat ccctatcagt
       61 gatagagata ctgagcacat cagcaggacg cactgaccga atttattaag gaggaggtga
      121 tccatgtcta gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag
      181 gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct
      241 acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg
      301 ttagataggc accatactca cttttgccct ttagaagggg aaagctggca agatttttta
      361 cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta
      421 catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt
      481 ttatgccaac aaggtttttc actagagaat gcattatatg cgctcagcgc tgtggggcat
      541 tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa
      601 acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac
      661 caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa
      721 caacttaaat gtgaaagtgg gtcttaataa taataattcc gaaggaggag gtgatccatg
      781 aattcagagc cgccgccgaa gctcgtcctt gacctggagc gcctcgcgca cgtcccccag
      841 gagaaggcgg gcccgctcca gcgctacgcg gcgacgatcc agtcgcagcg cggcgactac
      901 aacggcaagg tgctgtcgat ccgtcaggac gacctgcgca ccctggccgt gatctacgac
      961 cagtcgccct cggtcctcac cgagcagctg atcagctggg gcgtcctcga cgcggacgcg
     1021 cgccgcgccg tcgcccacga ggagaacccg aagaatatgc cggtcccgta taacgtcttt
     1081 atcatggccg acaagcagaa gaacggcatc aaggcgaact tcaagatccg ccacaacatc
     1141 gaggacggcg gcgtgcagct cgcctatcac taccagcaga acacccccat cggcgacggc
     1201 cccgtgctgc tgcccgacaa ccactacctg ggcgtgcagt ccaaactgag caaagacccc
     1261 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     1321 ggcatggacg agctgtacaa gggcggtacc ggagggagca tggtgagcaa gggcgaggag
     1381 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     1441 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     1501 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     1561 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     1621 gccatgcccg aaggctacat tcaggagcgc accatcttct tcaaggacga cggcaactat
     1681 aagacacgcg ctgaggttaa gttcgagggc gacactctgg ttaaccgcat cgagctgaag
     1741 ggcatcgact tcaaggagga cggcaacatc ctgggccata agcttgaata taacatgttg
     1801 tcggagccgg agccgccgcc gaagctcgtc cttgacctgg agcgcctcgc gcacgtcccc
     1861 caggagaagg cgggcccgct ccagcgctac gcggcgacga tccagtcgca gcgcggcgac
     1921 tacaacggca aggtgctgtc gatccgtcag gacgacctgc gcaccctggc cgtgatctac
     1981 gaccagtcgc cctcggtcct caccgagcag ctgatcagct ggggcgtcct cgacgcggac
     2041 gcgcgccgcg ccgtcgccca cgaggagaac tgaactagtg acgtcaggtg gcacttttcg
     2101 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     2161 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     2221 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     2281 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     2341 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     2401 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt
     2461 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     2521 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     2581 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     2641 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     2701 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc
     2761 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     2821 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     2881 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg gctctcgcgg
     2941 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     3001 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     3061 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     3121 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     3181 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     3241 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     3301 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3361 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     3421 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3481 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3541 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     3601 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     3661 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     3721 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     3781 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     3841 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
//
