LOCUS       sequence-437161-        4637 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cdGreen-Matry expressed from an aTc-inducible promoter (Internal Lab
            ID: UJ11208).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Kaczmarczyk A, van Vliet S, Jakob RP, Teixeira RD, Scheidat I, 
            Reinders A, Klotz A, Maier T, Jenal U
  TITLE     A genetically encoded biosensor to monitor dynamic changes of 
            c-di-GMP with high temporal resolution.
  JOURNAL   Nat Commun. 2024 May 9;15(1):3920. doi: 10.1038/s41467-024-48295-0.
  PUBMED    38724508
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-05-9"; volume: "15(1):3920. doi"; pages: " 
            10.1038/s41467-024-48295-0"
COMMENT     Sequence Label: p2H12Matry
FEATURES             Location/Qualifiers
     source          1..4637
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(57..75,76..81,82..98,99..104,105..130)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    57..75
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    82..100
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             156..779
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(229..248)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     1310..1331
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2184..2205)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        2890..2994
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2995..3063,3064..3855)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3213..3232)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4026..4614
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4515..4534
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acggttcctg gccttttgct ggccttttgc tcacatgttt tcgtcttcac ctcgagtccc
       61 tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac atcagcagga
      121 cgcactgacc gaatttatta aggaggaggt gatccatgtc tagattagat aaaagtaaag
      181 tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta
      241 aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc
      301 gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact cacttttgcc
      361 ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt tttagatgtg
      421 ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac
      481 agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga
      541 atgcattata tgcgctcagc gctgtggggc attttacttt aggttgcgta ttggaagatc
      601 aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat
      661 tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc ttcttattcg
      721 gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtcttaat
      781 aataataatt ccgaaggagg aggtgatcca tgaattcaga gccgccgccg aagctcgtcc
      841 ttgacctgga gcgcctcgcg cacgtccccc aggagaaggc gggcccgctc cagcgctacg
      901 cggcgacgat ccagtcgcag cgcggcgact acaacggcaa ggtgctgtcg atccgtcagg
      961 acgacctgcg caccctggcc gtgatctacg accagtcgcc ctcggtcctc accgagcagc
     1021 tgatcagctg gggcgtcctc gacgcggacg cgcgccgcgc cgtcgcccac gaggagaacc
     1081 cgaagaatat gccggtcccg tataacgtct ttatcatggc cgacaagcag aagaacggca
     1141 tcaaggcgaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag ctcgcctatc
     1201 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     1261 tgggcgtgca gtccaaactg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     1321 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggcggta
     1381 ccggaggcag cggcggcacg ggcggaagca agaaatatgg tgaagccgtg atcaaagaat
     1441 ttatgcgctt taaagttcac atggaaggta gcatgaatgg ccacgaattt gaaattgaag
     1501 gtgaaggcga aggtcgtccg tatgaaggca cccagaccgc aaaactgaaa gttaccaaag
     1561 gtggtccgct gccgtttagc tgggatattc tgagtccgca gtttatgtat ggtagccgtg
     1621 catttatcaa acatccggca gatattccgg attactataa acagagcttt ccggaaggtt
     1681 ttaaatggga acgtgtgatg aattttgaag atggtggtgc agttaccgtt acacaggata
     1741 ccagcctgga agatggcacc ctgatctata aagttaaact gcgtggcacc aattttccgc
     1801 ctgatggtcc ggttatgcag aaaaaaacca tgggttggga agcaagcacc gaacgtctgt
     1861 atcctgaaga tggcgttctg aaaggtgata tcaaaatggc actgcgtctg aaagatggcg
     1921 gtcgttatct ggcagatttc aaaaccacct acaaagccaa aaaaccggtt cagatgcctg
     1981 gtgcatataa tgttgatcgc aaactggata ttaccagcca caatgaagat tataccgtgg
     2041 tggaacagta tgaacgtagc gaaggccgtc atagtaccgg tggtatggat gaactgtaca
     2101 agggcgggag cggaggcacg ggaggtaccg gagggagcat ggtgagcaag ggcgaggagc
     2161 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     2221 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     2281 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     2341 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     2401 ccatgcccga aggctacatt caggagcgca ccatcttctt caaggacgac ggcaactata
     2461 agacacgcgc tgaggttaag ttcgagggcg acactctggt taaccgcatc gagctgaagg
     2521 gcatcgactt caaggaggac ggcaacatcc tgggccataa gcttgaatat aacatgttgt
     2581 cggagccgga gccgccgccg aagctcgtcc ttgacctgga gcgcctcgcg cacgtccccc
     2641 aggagaaggc gggcccgctc cagcgctacg cggcgacgat ccagtcgcag cgcggcgact
     2701 acaacggcaa ggtgctgtcg atccgtcagg acgacctgcg caccctggcc gtgatctacg
     2761 accagtcgcc ctcggtcctc accgagcagc tgatcagctg gggcgtcctc gacgcggacg
     2821 cgcgccgcgc cgtcgcccac gaggagaact gaactagtga cgtcaggtgg cacttttcgg
     2881 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     2941 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     3001 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     3061 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     3121 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     3181 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     3241 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     3301 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     3361 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     3421 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     3481 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca
     3541 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     3601 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     3661 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg ctctcgcggt
     3721 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     3781 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     3841 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     3901 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     3961 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     4021 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     4081 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     4141 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     4201 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     4261 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     4321 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     4381 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     4441 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     4501 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     4561 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     4621 agcaacgcgg ccttttt
//
