LOCUS       sequence-437166-        6316 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a 
            constitutive promoter in an operon with mScarlet-I (downstream) as a
            reference FP (Internal Lab ID: UJ11241).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Kaczmarczyk A, van Vliet S, Jakob RP, Teixeira RD, Scheidat I, 
            Reinders A, Klotz A, Maier T, Jenal U
  TITLE     A genetically encoded biosensor to monitor dynamic changes of 
            c-di-GMP with high temporal resolution.
  JOURNAL   Nat Commun. 2024 May 9;15(1):3920. doi: 10.1038/s41467-024-48295-0.
  PUBMED    38724508
REFERENCE   2  (bases 1 to 6316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-05-9"; volume: "15(1):3920. doi"; pages: " 
            10.1038/s41467-024-48295-0"
COMMENT     Sequence Label: pBBR15.2-2H12.D11opt-scarRef
FEATURES             Location/Qualifiers
     source          1..6316
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    267..288
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1032..1053)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1293..1312)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             2924..3457
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     CDS             complement(3860..4522)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      4523..5294
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctgacgtct aagaacatgt tgcagctggc acgacaggtt tcccgactgg
      241 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
      301 gctttatagc tagcttgaca tcccatagag tgatagagat actgagcaca tcagcatatg
      361 aaagtgggtc ttaataataa taattccgaa ggaggaggtg atccatgaat tcggaaccgc
      421 cgccgaagct ggtcctggac ctggaacgcc tggcgcacgt cccgcaggaa aaggcgggcc
      481 cgctgcagcg ctatgccgcc accatccagt cgcagcgggg cgactacaac ggcaaggtcc
      541 tgtcgatccg gccggcggac ctgcgcaccc tggccgtcat ctatgaccag tcgccgtcgg
      601 tcctgaccga gcagctgatc tcgtggggcg tcctggatgc cgatgcgcgc cgcgccgtcg
      661 cgcatgaaga gaacccgaag aacatgccgg tgccgtacaa cgtgttcatc atggccgaca
      721 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gatggcggcg
      781 tccagctggc ctaccactac cagcagaaca ccccgatcgg cgacggcccg gtcctgctgc
      841 cggacaacca ttacctgggc gtccagtcga agctgtcgaa ggacccgaac gagaagcgcg
      901 accacatggt gctgctggag ttcgtcaccg ccgcgggcat caccctgggc atggacgagc
      961 tgtacaaggg cggcaccggc ggctcgggcg tcagcaaggg cgaagaactg ttcaccggcg
     1021 tcgtcccgat cctggtcgag ctggacggcg acgtcaacgg ccacaagttc tcggtcagcg
     1081 gcgaaggcga gggcgacgcc acctatggca agctgaccct gaagttcatc tgcaccaccg
     1141 gcaagctgcc cgtgccgtgg ccgacgctgg tcaccacgct gacctatggc gtgcagtgct
     1201 tctcgcgcta cccggaccac atgaagcagc acgatttctt caagagcgcc atgccggaag
     1261 gctacatcca ggaacggacc atcttcttca aggacgacgg caactacaag acccgcgccg
     1321 aggtcaagtt cgaaggcgac accctggtca accgcatcga gctgaagggc atcgacttca
     1381 aggaagatgg caacatcctg ggccacaagc tggaatacaa catgctgtcg gagcccgaac
     1441 cgccgccgaa gctggtgctg gatctggaac ggctggccca tgtgccgcag gaaaaggcgg
     1501 gcccgctgca gcggtatgcg gcgacgatcc agagccagcg cggcgattat aacggcaagg
     1561 tgctgagcat ccggccggcg gatctgcgga cgctggcggt gatctacgat cagagcccga
     1621 gcgtgctgac ggaacagctg atctcctggg gcgtgctgga cgcggatgcc cggcgcgcgg
     1681 tcgcccacga ggaaaactaa actagtatga gcaagaaata tggtgaagcc gtgatcaaag
     1741 aatttatgcg ctttaaagtt cacatggaag gtagcatgaa tggccacgaa tttgaaattg
     1801 aaggtgaagg cgaaggtcgt ccgtatgaag gcacccagac cgcaaaactg aaagttacca
     1861 aaggtggtcc gctgccgttt agctgggata ttctgagtcc gcagtttatg tatggtagcc
     1921 gtgcatttat caaacatccg gcagatattc cggattacta taaacagagc tttccggaag
     1981 gttttaaatg ggaacgtgtg atgaattttg aagatggtgg tgcagttacc gttacacagg
     2041 ataccagcct ggaagatggc accctgatct ataaagttaa actgcgtggc accaattttc
     2101 cgcctgatgg tccggttatg cagaaaaaaa ccatgggttg ggaagcaagc accgaacgtc
     2161 tgtatcctga agatggcgtt ctgaaaggtg atatcaaaat ggcactgcgt ctgaaagatg
     2221 gcggtcgtta tctggcagat ttcaaaacca cctacaaagc caaaaaaccg gttcagatgc
     2281 ctggtgcata taatgttgat cgcaaactgg atattaccag ccacaatgaa gattataccg
     2341 tggtggaaca gtatgaacgt agcgaaggcc gtcatagtac cggtggtatg gatgaactgt
     2401 acaagtgagc tagtaaaaaa ggatccagct tcacgctgcc gcaagcactc agggcgcaag
     2461 ggctgctaaa ggaagcggaa cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg
     2521 atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag
     2581 gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc
     2641 gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac
     2701 tggatggctt tcttgccgcc aaggatctga tggcgcaggg gatcaagatc catgcgctca
     2761 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc
     2821 atggcttgtt atgactgttt ttttgtacag tctattcctc gggcatccaa gcagcaagcg
     2881 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagcaa
     2941 cgatgttacg cagcagggca gtcgccctaa aacaaagtta ggtggctcaa gtatgggcat
     3001 cattcgcaca tgtaggctcg gccctgacca agtcaaatcc atgcgggctg ctcttgatct
     3061 tttcggtcgt gagttcggag acgtagccac ctactcccaa catcagccgg actccgatta
     3121 cctcgggaac ttgctccgta gtaagacatt catcgcgctt gctgccttcg accaagaagc
     3181 ggttgttggc gctctcgcgg cttacgttct gcccaagttt gagcagccgc gtagtgagat
     3241 ctatatctat gatctcgcag tctccggcga gcaccggagg cagggcattg ccaccgcgct
     3301 catcaatctc ctcaagcatg aggccaacgc gcttggtgct tatgtgatct acgtgcaagc
     3361 agattacggt gacgatcccg cagtggctct ctatacaaag ttgggcatac gggaagaagt
     3421 gatgcacttt gatatcgacc caagtaccgc cacctaacaa ttcgttcaag ccgagatcgg
     3481 cttcccggcc gcggagttgt tacttgttcg gttcagggca gggtcgttaa atagccgctt
     3541 atgtctattg ctggtttacc ggtttattga ctaccggaag cagtgtgacc gtgtgcttct
     3601 caaatgcctg aggccagttt gctcaggctc tccccgtgga ggtaataatt gacgatatga
     3661 tcatttattc tgcctcccag agcctgataa aaacggtgaa tccgttagcg aggtgccgcc
     3721 ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca
     3781 gacaaggtat agggcggcga ggcggctaca gccgatagtc tggaacagcg cacttacggg
     3841 ttgctgcgca acccaagtgc taccggcgcg gcagcgtgac ccgtgtcggc ggctccaacg
     3901 gctcgccatc gtccagaaaa cacggctcat cgggcatcgg caggcgctgc tgcccgcgcc
     3961 gttcccattc ctccgtttcg gtcaaggctg gcaggtctgg ttccatgccc ggaatgccgg
     4021 gctggctggg cggctcctcg ccggggccgg tcggtagttg ctgctcgccc ggatacaggg
     4081 tcgggatgcg gcgcaggtcg ccatgcccca acagcgattc gtcctggtcg tcgtgatcaa
     4141 ccaccacggc ggcactgaac accgacaggc gcaactggtc gcggggctgg ccccacgcca
     4201 cgcggtcatt gaccacgtag gccgacacgg tgccggggcc gttgagcttc acgacggaga
     4261 tccagcgctc ggccaccaag tccttgactg cgtattggac cgtccgcaaa gaacgtccga
     4321 tgagcttgga aagtgtcttc tggctgacca ccacggcgtt ctggtggccc atctgcgcca
     4381 cgaggtgatg cagcagcatt gccgccgtgg gtttcctcgc aataagcccg gcccacgcct
     4441 catgcgcttt gcgttccgtt tgcacccagt gaccgggctt gttcttggct tgaatgccga
     4501 tttctctgga ctgcgtggcc atgcttatct ccatgcggta ggagtgccgc acggttgcgg
     4561 caccatgcgc aatcagctgc aactcttcgg cagcgcgaca acaattatgc gttgcgtaaa
     4621 agtggcagtc aattacagat tttctttaac ctacgcaatg agctattgcg gggggtgccg
     4681 caatgagctg ttgcgtaccc ccctttttta agttgttgat ttttaagtct ttcgcatttc
     4741 gccctatatc tagttctttg gtgcccaaag aagggcaccc ctgcggggtt cccccacgcc
     4801 ttcggcgcgg ctccccctcc ggcaaaaagt ggcccctccg gggcttgttg atcgactgcg
     4861 cggccttcgg ccttgcccaa ggtggcgctg cccccttgga acccccgcac tcgccgccgt
     4921 gaggctcggg gggcaggcgg gcgggcttcg cccttcgact gcccccactc gcataggctt
     4981 gggtcgttcc aggcgcgtca aggccaagcc gctgcgcggt cgctgcgcga gccttgaccc
     5041 gccttccact tggtgtccaa ccggcaagcg aagcgcgcag gccgcaggcc ggaggctttt
     5101 ccccagagaa aattaaaaaa attgatgggg caaggccgca ggccgcgcag ttggagccgg
     5161 tgggtatgtg gtcgaaggct gggtagccgg tgggcaatcc ctgtggtcaa gctcgtgggc
     5221 aggcgcagcc tgtccatcag cttgtccagc agggttgtcc acgggccgag cgaagcgagc
     5281 cagccggtgg ccgctcgcgg ccatcgtcca catatccacg ggctggcaag ggagcgcagc
     5341 gaccgcgcag ggcgaagccc ggagagcaag cccgtagggc gccgcagccg ccgtaggcgg
     5401 tcacgacttt gcgaagcaaa gtctagtgag tatactcaag cattgagtgg cccgccggag
     5461 gcaccgcctt gcgctgcccc cgtcgagccg gttggacacc aaaagggagg ggcaggcatg
     5521 gcggcatacg cgatcatgcg atgcaagaag ctggcgaaaa tgggcaacgt ggcggccagt
     5581 ctcaagcacg cctaccgcga gcgcgagacg cccaacgctg acgccagcag gacgccagag
     5641 aacgagcact gggcggccag cagcaccgat gaagcgatgg gccgactgcg cgagttgctg
     5701 ccagagaagc ggcgcaagga cgctgtgttg gcggtcgagt acgtcatgac ggccagcccg
     5761 gaatggtgga agtcggccag ccaagaacag caggcggcgt tcttcgagaa ggcgcacaag
     5821 tggctggcgg acaagtacgg ggcggatcgc atcgtgacgg ccagcatcca ccgtgacgaa
     5881 accagcccgc acatgaccgc gttcgtggtg ccgctgacgc aggacggcag gctgtcggtc
     5941 aaggagttca tcgacaacaa agcgcagatg acccgcgacc agaccacgtt tgcggccgct
     6001 gtggccgatc tagggctgca acggggcatc gagggcagca aggcacgtca cacgcgcatt
     6061 caggcgttct acgaggccct ggagcggcca ccagtgggcc acgtcaccat cagcccgcaa
     6121 gcggtcgagc cacgcgccta tgcaccgcag ggattggccg aaaagctggg aatctcaaag
     6181 cgcgttgaga cgccggaagc cgtggccgac cggctgacaa aagcggttcg gcaggggtat
     6241 gagcctgccc tacaggccgc cgcaggagcg cgtgagatgc gcaagaaggc cgatcaagcc
     6301 caagagacgg cccgag
//
