LOCUS       sequence-437176-        4637 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cdGreen-Matry(blind) expressed from an aTc-inducible promoter 
            (Internal Lab ID: UJ11209).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Kaczmarczyk A, van Vliet S, Jakob RP, Teixeira RD, Scheidat I, 
            Reinders A, Klotz A, Maier T, Jenal U
  TITLE     A genetically encoded biosensor to monitor dynamic changes of 
            c-di-GMP with high temporal resolution.
  JOURNAL   Nat Commun. 2024 May 9;15(1):3920. doi: 10.1038/s41467-024-48295-0.
  PUBMED    38724508
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-05-9"; volume: "15(1):3920. doi"; pages: " 
            10.1038/s41467-024-48295-0"
COMMENT     Sequence Label: p2H12Matry-blind
FEATURES             Location/Qualifiers
     source          1..4637
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(25..43,44..49,50..66,67..72,73..98)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    25..43
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    50..68
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             124..747
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(197..216)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     1278..1299
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2152..2173)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        2858..2962
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2963..3031,3032..3823)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3181..3200)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3994..4582
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4483..4502
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acatgttttc gtcttcacct cgagtcccta tcagtgatag agattgacat ccctatcagt
       61 gatagagata ctgagcacat cagcaggacg cactgaccga atttattaag gaggaggtga
      121 tccatgtcta gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag
      181 gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct
      241 acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg
      301 ttagataggc accatactca cttttgccct ttagaagggg aaagctggca agatttttta
      361 cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta
      421 catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt
      481 ttatgccaac aaggtttttc actagagaat gcattatatg cgctcagcgc tgtggggcat
      541 tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa
      601 acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac
      661 caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa
      721 caacttaaat gtgaaagtgg gtcttaataa taataattcc gaaggaggag gtgatccatg
      781 aattcagagc cgccgccgaa gctcgtcctt gacctggagc gcctcgcgca cgtcccccag
      841 gagaaggcgg gcccgctcca gcgctacgcg gcgacgatcc agtcgcagga cggccgctac
      901 aacggcaagg tgctgtcgat ccgtcaggac gacctgcgca ccctggccgt gatctacgac
      961 cagtcgccct cggtcctcac cgagcagctg atcagctggg gcgtcctcga cgcggacgcg
     1021 cgccgcgccg tcgcccacga ggagaacccg aagaatatgc cggtcccgta taacgtcttt
     1081 atcatggccg acaagcagaa gaacggcatc aaggcgaact tcaagatccg ccacaacatc
     1141 gaggacggcg gcgtgcagct cgcctatcac taccagcaga acacccccat cggcgacggc
     1201 cccgtgctgc tgcccgacaa ccactacctg ggcgtgcagt ccaaactgag caaagacccc
     1261 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     1321 ggcatggacg agctgtacaa gggcggtacc ggaggcagcg gcggcacggg cggaagcaag
     1381 aaatatggtg aagccgtgat caaagaattt atgcgcttta aagttcacat ggaaggtagc
     1441 atgaatggcc acgaatttga aattgaaggt gaaggcgaag gtcgtccgta tgaaggcacc
     1501 cagaccgcaa aactgaaagt taccaaaggt ggtccgctgc cgtttagctg ggatattctg
     1561 agtccgcagt ttatgtatgg tagccgtgca tttatcaaac atccggcaga tattccggat
     1621 tactataaac agagctttcc ggaaggtttt aaatgggaac gtgtgatgaa ttttgaagat
     1681 ggtggtgcag ttaccgttac acaggatacc agcctggaag atggcaccct gatctataaa
     1741 gttaaactgc gtggcaccaa ttttccgcct gatggtccgg ttatgcagaa aaaaaccatg
     1801 ggttgggaag caagcaccga acgtctgtat cctgaagatg gcgttctgaa aggtgatatc
     1861 aaaatggcac tgcgtctgaa agatggcggt cgttatctgg cagatttcaa aaccacctac
     1921 aaagccaaaa aaccggttca gatgcctggt gcatataatg ttgatcgcaa actggatatt
     1981 accagccaca atgaagatta taccgtggtg gaacagtatg aacgtagcga aggccgtcat
     2041 agtaccggtg gtatggatga actgtacaag ggcgggagcg gaggcacggg aggtaccgga
     2101 gggagcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     2161 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     2221 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     2281 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     2341 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacattca ggagcgcacc
     2401 atcttcttca aggacgacgg caactataag acacgcgctg aggttaagtt cgagggcgac
     2461 actctggtta accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     2521 ggccataagc ttgaatataa catgttgtcg gagccggagc cgccgccgaa gctcgtcctt
     2581 gacctggagc gcctcgcgca cgtcccccag gagaaggcgg gcccgctcca gcgctacgcg
     2641 gcgacgatcc agtcgcagcg cggcgactac aacggcaagg tgctgtcgat ccgtcaggac
     2701 gacctgcgca ccctggccgt gatctacgac cagtcgccct cggtcctcac cgagcagctg
     2761 atcagctggg gcgtcctcga cgcggacgcg cgccgcgccg tcgcccacga ggagaactga
     2821 actagtgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     2881 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     2941 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     3001 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     3061 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     3121 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     3181 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     3241 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     3301 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     3361 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     3421 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     3481 cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac
     3541 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     3601 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     3661 tggagccggt gagcgtggct ctcgcggtat cattgcagca ctggggccag atggtaagcc
     3721 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     3781 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     3841 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     3901 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     3961 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4021 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4081 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4141 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     4201 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     4261 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     4321 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     4381 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     4441 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     4501 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4561 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4621 ttgctggcct tttgctc
//
