LOCUS       sequence-437177-        3960 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cdGreen expressed from a constitutive promoter in an operon with 
            mScarlet-I (downstream) as a reference FP (Internal Lab ID: 
            UJ11213).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Kaczmarczyk A, van Vliet S, Jakob RP, Teixeira RD, Scheidat I, 
            Reinders A, Klotz A, Maier T, Jenal U
  TITLE     A genetically encoded biosensor to monitor dynamic changes of 
            c-di-GMP with high temporal resolution.
  JOURNAL   Nat Commun. 2024 May 9;15(1):3920. doi: 10.1038/s41467-024-48295-0.
  PUBMED    38724508
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-05-9"; volume: "15(1):3920. doi"; pages: " 
            10.1038/s41467-024-48295-0"
COMMENT     Sequence Label: pConRef-2H12
FEATURES             Location/Qualifiers
     source          1..3960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     640..661
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(767..788)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        2181..2285
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2286..2354,2355..3146)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2504..2523)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3317..3905
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3806..3825
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acatgttttc gtcttcacct cgattgacat cccatagagt gatagagata ctgagcacat
       61 cagcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtcttaat aataataatt
      121 ccgaaggagg aggtgatcca tgaattcaga gccgccgccg aagctcgtcc ttgacctgga
      181 gcgcctcgcg cacgtccccc aggagaaggc gggcccgctc cagcgctacg cggcgacgat
      241 ccagtcgcag cgcggcgact acaacggcaa ggtgctgtcg atccgtcagg acgacctgcg
      301 caccctggcc gtgatctacg accagtcgcc ctcggtcctc accgagcagc tgatcagctg
      361 gggcgtcctc gacgcggacg cgcgccgcgc cgtcgcccac gaggagaacc cgaagaatat
      421 gccggtcccg tataacgtct ttatcatggc cgacaagcag aagaacggca tcaaggcgaa
      481 cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag ctcgcctatc actaccagca
      541 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgggcgtgca
      601 gtccaaactg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
      661 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggcggta ccggagggag
      721 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      781 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      841 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      901 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
      961 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac attcaggagc gcaccatctt
     1021 cttcaaggac gacggcaact ataagacacg cgctgaggtt aagttcgagg gcgacactct
     1081 ggttaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctgggcca
     1141 taagcttgaa tataacatgt tgtcggagcc ggagccgccg ccgaagctcg tccttgacct
     1201 ggagcgcctc gcgcacgtcc cccaggagaa ggcgggcccg ctccagcgct acgcggcgac
     1261 gatccagtcg cagcgcggcg actacaacgg caaggtgctg tcgatccgtc aggacgacct
     1321 gcgcaccctg gccgtgatct acgaccagtc gccctcggtc ctcaccgagc agctgatcag
     1381 ctggggcgtc ctcgacgcgg acgcgcgccg cgccgtcgcc cacgaggaga actgaactag
     1441 tatgagcaag aaatatggtg aagccgtgat caaagaattt atgcgcttta aagttcacat
     1501 ggaaggtagc atgaatggcc acgaatttga aattgaaggt gaaggcgaag gtcgtccgta
     1561 tgaaggcacc cagaccgcaa aactgaaagt taccaaaggt ggtccgctgc cgtttagctg
     1621 ggatattctg agtccgcagt ttatgtatgg tagccgtgca tttatcaaac atccggcaga
     1681 tattccggat tactataaac agagctttcc ggaaggtttt aaatgggaac gtgtgatgaa
     1741 ttttgaagat ggtggtgcag ttaccgttac acaggatacc agcctggaag atggcaccct
     1801 gatctataaa gttaaactgc gtggcaccaa ttttccgcct gatggtccgg ttatgcagaa
     1861 aaaaaccatg ggttgggaag caagcaccga acgtctgtat cctgaagatg gcgttctgaa
     1921 aggtgatatc aaaatggcac tgcgtctgaa agatggcggt cgttatctgg cagatttcaa
     1981 aaccacctac aaagccaaaa aaccggttca gatgcctggt gcatataatg ttgatcgcaa
     2041 actggatatt accagccaca atgaagatta taccgtggtg gaacagtatg aacgtagcga
     2101 aggccgtcat agtaccggtg gtatggatga actgtacaag tgagctagcg acgtcaggtg
     2161 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     2221 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     2281 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     2341 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     2401 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     2461 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     2521 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     2581 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     2641 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     2701 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     2761 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     2821 cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     2881 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     2941 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     3001 gctctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     3061 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     3121 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     3181 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     3241 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     3301 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3361 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3421 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     3481 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3541 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3601 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3661 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     3721 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3781 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3841 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     3901 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
//
