LOCUS       sequence-437186-        4275 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Wolf G, Craigon C, Teoh ST, Essletzbichler P, Onstein S, Cassidy D, 
            Uijttewaal ECH, Dvorak V, Cao Y, Bensimon A, Elling U, Ciulli A, 
            Superti-Furga G
  TITLE     The efflux pump ABCC1/MRP1 constitutively restricts PROTAC 
            sensitivity in cancer cells.
  JOURNAL   Cell Chem Biol. 2024 Dec 24:S2451-9456(24)00489-6. doi: 
            10.1016/j.chembiol.2024.11.009.
  PUBMED    39755121
REFERENCE   2  (bases 1 to 4275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Chem 
            Biol. 2024 Dec 24:S2451-9456(24)00489-6. doi: 
            10.1016/j.chembiol.2024.11.009."
COMMENT     Sequence Label: pENTR-ABCF2_STOP
FEATURES             Location/Qualifiers
     source          1..4275
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2011..2110)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2167..2183)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2167..2183)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2180..2202)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2355..2943)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2435..2454)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3079..3894)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3079..3894)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3802..3821
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3886..3905
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     4015..4031
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4015..4031
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      4032..4078
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4192..4214
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4206..4223
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4207..4223
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacagat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct gccaccatgc ccagcgacct
      121 ggctaagaag aaagctgcca agaagaaaga ggcagcgaaa gcgcgccaaa gaccgaggaa
      181 ggggcacgag gaaaacggag atgtagtgac tgaaccgcaa gtggctgaga agaacgaggc
      241 taacggccga gagactaccg aggttgatct cttgactaag gaattggaag atttcgaaat
      301 gaagaaggcc gcagcaagag ccgtaacagg cgtgctcgcg tctcatccta actctacaga
      361 tgtacatata attaacctta gtctgacctt tcacggccag gagctcctga gcgatacaaa
      421 actcgagctt aactccggcc gtaggtacgg gttgatcggt ttgaatggca ttggaaaatc
      481 tatgctcttg tccgcgatcg gaaaacgcga ggtcccaatt ccggagcaca tcgatatcta
      541 ccatctgaca agggaaatgc caccatctga taagactccc ttgcactgtg ttatggaagt
      601 ggacactgaa cgagcgatgt tagaaaagga agccgagaga ctggcccacg aagacgctga
      661 atgcgaaaag ttgatggaac tttatgagcg gctggaagag ctggatgctg acaaagccga
      721 gatgagagcg agcagaattc tgcatggact tggctttacc cctgctatgc agagaaagaa
      781 gctgaaagac ttcagtggcg gttggcgtat gcgcgttgcc cttgctaggg ctttgttcat
      841 tagacctttt atgttgttgc tcgatgaacc cacaaatcac ctcgatttgg acgcttgcgt
      901 ctggcttgaa gaggagctga agacgtttaa gcgcattctc gttctggttt cccacagcca
      961 agattttctg aacggagtct gcacaaacat tatccatatg cacaacaaga agctgaagta
     1021 ttatactggg aattacgatc agtacgttaa gacaagactg gagctcgagg agaaccaaat
     1081 gaaacgattc cactgggagc aagaccaaat tgctcatatg aagaactata tcgctcggtt
     1141 tgggcatggg tcagctaaat tggcccgcca agcgcagtct aaagagaaaa ctttgcaaaa
     1201 gatgatggcg agcggactga cagaacgggt cgtgtctgat aagacactct ccttctattt
     1261 tcccccatgc ggtaaaattc ctcctccggt cattatggtg caaaatgtca gctttaaata
     1321 caccaaggat gggccatgta tctataacaa cctcgaattt ggaatcgacc tcgatacacg
     1381 cgtggcactg gtgggaccga acggggccgg caagtcaacc ctgcttaagc tgctcaccgg
     1441 ggaactgtta cctaccgatg gaatgattag aaaacatagt catgtgaaga ttgggcggta
     1501 ccatcaacac cttcaagaac aacttgacct cgatttgtca ccattggagt acatgatgaa
     1561 atgttatcct gagataaaag agaaggaaga gatgcggaaa attattggga gatatggatt
     1621 gacaggcaag caacaagtct cacctatacg aaatctttct gacggacaaa agtgccgggt
     1681 ctgcttagcg tggctcgcct ggcagaaccc tcatatgctg tttctggatg aacccacaaa
     1741 ccacttagat attgagacca ttgacgccct tgccgacgca atcaacgaat ttgagggcgg
     1801 gatgatgctc gtgagccacg actttcgcct catccaacaa gtagcgcaag aaatttgggt
     1861 gtgcgaaaag cagactatta caaaatggcc cggcgatatt ctcgcctaca aggagcactt
     1921 gaaatcaaaa ttggtagacg aggaacccca actcaccaag cgcacacaca acgtctaact
     1981 tgtggaaagg acgtgtcaca tagtcttgcg acccagcttt cttgtacaaa gttggcatta
     2041 taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     2101 tcattatttg aggctaggtg gaggctcagt gatgataagt ctgcgatggt ggatgcatgt
     2161 gtcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcagagggc acaatcctat
     2221 tccgcgctat ccgacaatct ccaagacatt aggtggagtt cagttcggcg tatggcatat
     2281 gtcgctggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     2341 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     2401 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2461 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2521 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     2581 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     2641 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     2701 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     2761 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     2821 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     2881 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     2941 caagaagatc ctttgatctt ttctacgggg tctgacgctc tattcaacaa agccgccgtc
     3001 ccgtcaagtc agcgtaaatg ggtagggggc ttcaaatcgt ccgctctgcc agtgttacaa
     3061 ccaattaaca aattctgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
     3121 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
     3181 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
     3241 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
     3301 atcaccatga gtgacgactg aatccggtga gaatggcaaa agcttatgca tttctttcca
     3361 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
     3421 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca
     3481 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
     3541 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt
     3601 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
     3661 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
     3721 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt
     3781 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
     3841 gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt tgaatatggc tcataacacc
     3901 ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgatgata tatttttatc
     3961 ttgtgcaatg taacatcaga gattttgaga cacaacgtgg ctttcccccg ccgctctaga
     4021 actagtggat ccaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     4081 gttgtttgtc gcattatacg agacgtccag gttgggatac ctgaaacaaa acccatcgta
     4141 cggccaagga agtctccaat aactgtgatc caccacaagc gccagggttt tcccagtcac
     4201 gacgttgtaa aacgacggcc agtcatgcat aatccgcacg catctggaat aaggaagtgc
     4261 cattccgcct gacct
//
