LOCUS       sequence-437188-        6246 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Wolf G, Craigon C, Teoh ST, Essletzbichler P, Onstein S, Cassidy D, 
            Uijttewaal ECH, Dvorak V, Cao Y, Bensimon A, Elling U, Ciulli A, 
            Superti-Furga G
  TITLE     The efflux pump ABCC1/MRP1 constitutively restricts PROTAC 
            sensitivity in cancer cells.
  JOURNAL   Cell Chem Biol. 2024 Dec 24:S2451-9456(24)00489-6. doi: 
            10.1016/j.chembiol.2024.11.009.
  PUBMED    39755121
REFERENCE   2  (bases 1 to 6246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Chem 
            Biol. 2024 Dec 24:S2451-9456(24)00489-6. doi: 
            10.1016/j.chembiol.2024.11.009."
COMMENT     Sequence Label: pENTR-ABCB1_STOP
FEATURES             Location/Qualifiers
     source          1..6246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      101..110
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3982..4081)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4138..4154)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4138..4154)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4151..4173)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(4326..4914)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4406..4425)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5050..5865)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5050..5865)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5773..5792
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5857..5876
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     5986..6002
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5986..6002
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      6003..6049
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     6163..6185
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6177..6194
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6178..6194
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacagat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct gccaccatgg acttagaagg
      121 ggatcggaac ggtggagcta agaagaagaa cttcttcaag ttgaacaaca agtcagagaa
      181 agataagaaa gaaaagaagc caacggtctc cgtgttctct atgttccgtt attccaattg
      241 gcttgacaaa ctgtacatgg tcgttgggac actcgctgct attatccatg gagccggcct
      301 tcctttgatg atgttggtgt tcggcgagat gacagacatc tttgccaacg cgggaaatct
      361 cgaggattta atgtcaaata tcaccaatcg gagtgatatc aatgacactg ggttcttcat
      421 gaatttggaa gaagatatga cgaggtatgc atattattac tccgggatcg gcgccggcgt
      481 cctcgtggca gcatatattc aagtgtcatt ttggtgcctc gccgcaggga gacaaataca
      541 caaaatcaga aaacaattct ttcacgcgat tatgaggcaa gaaataggtt ggtttgacgt
      601 ccacgatgtc ggggaactga acacaagatt aacggacgac gtcagcaaaa tcaatgaagg
      661 gattggggat aaaattggca tgttctttca atctatggct actttcttca ctgggtttat
      721 cgtcggtttc actagaggtt ggaagcttac acttgtcatt cttgctatca gtcccgtgct
      781 cgggctcagt gcggccgtct gggccaagat cttgagcagt ttcacggata aagaattact
      841 ggcatacgcg aaagctggtg cagtggccga agaggtcttg gcagccatta gaacagtgat
      901 agctttcggc ggacaaaaga aggaattaga gagatacaat aagaaccttg aagaggctaa
      961 gcgcattggt atcaagaagg ccataaccgc taatatttca atcggggcgg cctttctgtt
     1021 aatctatgca agctatgcgt tggcattttg gtacggtact acgttagtgc ttagtgggga
     1081 gtattccatc ggtcaggttc tgacagtatt cttctctgtt ctgatagggg cattttcagt
     1141 gggccaagca agtccaagca ttgaggcttt tgcgaacgct agaggcgcag cctacgagat
     1201 ctttaaaatt atagataaca agcccagcat tgacagttat tccaagagcg gtcataagcc
     1261 agacaatata aaaggcaatt tggaatttcg gaatgttcac tttagctatc ctagccgaaa
     1321 ggaagtgaag attttgaaag gtctgaactt aaaagtccag agtggccaaa ccgtggcact
     1381 ggtcggaaac agcggatgtg ggaaaagcac gaccgtgcaa ctgatgcaac gcctttacga
     1441 ccctaccgag ggaatggtct ccgttgacgg tcaagatatc aggaccatta acgtgcgatt
     1501 tctccgcgag atcattggcg tagtttcaca agaacccgta ctcttcgcca caaccatagc
     1561 cgaaaacata agatatgggc gtgaaaatgt cacaatggat gaaatagaaa aggccgtgaa
     1621 agaagccaat gcgtacgact tcattatgaa actgcctcat aaatttgaca cgctggtcgg
     1681 ggagcgtgga gcgcagttaa gcggcggaca aaagcaacgg atcgctatcg ctcgagcact
     1741 ggttcgaaat ccaaagattc tcctgttaga tgaagccacg tccgccctgg acactgaatc
     1801 agaggccgtc gtccaggtgg ccttggataa agcacgcaaa gggcggacca cgatcgtgat
     1861 agcccaccgg ttgtcaacag tgcggaatgc ggacgtcatt gcaggttttg acgacggcgt
     1921 gattgtcgaa aagggtaatc atgacgagtt aatgaaggag aaaggcattt attttaaatt
     1981 ggtcaccatg caaacggcag gtaacgaagt ggaactggaa aacgctgccg acgaatccaa
     2041 gtctgagata gacgccctcg aaatgtcctc taacgattca agaagtagcc tgattagaaa
     2101 acggtccacc cgacgctccg ttcgtgggtc ccaagcacag gaccggaagc tgagcaccaa
     2161 agaggcctta gatgagtcaa tcccgccggt ctctttctgg cgcatcatga agcttaatct
     2221 caccgaatgg ccctatttcg tcgtgggcgt tttctgtgct attatcaacg gcgggcttca
     2281 gccggcattt gccataattt tctccaaaat catcggggtc tttactcgga tagatgaccc
     2341 tgaaactaaa aggcaaaatt ctaacctgtt cagccttctt ttcctcgctc tgggtatcat
     2401 cagctttata acattcttcc tgcagggatt cacgtttgga aaagccgggg agattctgac
     2461 aaagcgactt aggtacatgg tgttcagaag catgctgagg caagatgtgt catggtttga
     2521 cgatcccaag aacacaactg gagcgctgac tacccgctta gcaaacgacg ctgctcaagt
     2581 taagggcgct attggctcta gattagccgt cataacacaa aacatcgcaa atctgggaac
     2641 cggcatcatt atttctttta tctatggctg gcaactcacc ctgcttctcc tggccattgt
     2701 gccgattata gcaatcgctg gtgttgtaga aatgaagatg ttatctgggc aagcattaaa
     2761 ggacaagaag gagctggaag ggtcaggaaa gattgccaca gaagccatcg agaactttag
     2821 gacagtagtg agcctgacgc aagagcagaa gttcgaacat atgtatgccc aatcattgca
     2881 agtgccttat agaaatagtt tgcgaaaagc acacatcttc ggaataacat ttagcttcac
     2941 ccaggcaatg atgtatttta gctatgccgg ttgcttcaga tttggagcct acctcgtggc
     3001 gcataaattg atgtcttttg aggacgtttt actcgtgttt tctgctgtcg tgtttggtgc
     3061 aatggccgta ggtcaagttt ccagtttcgc ccctgactat gccaaagcca agataagcgc
     3121 agctcacatc atcatgatca tcgagaaaac ccctctgatc gattcctatt ccactgaagg
     3181 tctcatgccc aatactctcg aagggaatgt gacattcgga gaggtcgttt tcaactaccc
     3241 aacgcggccg gatattccgg ttctgcaggg attgtcttta gaggtcaaga aggggcaaac
     3301 gctcgcgctc gtcgggagta gtggctgtgg caaaagtacc gttgtccaat tacttgaacg
     3361 cttctacgat cctctggcgg gcaaagtgct cctcgatgga aaagaaatca aacggttaaa
     3421 cgttcaatgg ctcagagccc atttaggcat tgtgagtcaa gagcccatcc tcttcgattg
     3481 ctctattgcc gaaaatatcg catatgggga taattcccgc gtcgtgtctc aagaagaaat
     3541 agtgcgtgca gccaaagaag ccaatattca tgcctttatt gaatcattac ccaacaaata
     3601 ctcaacaaag gtcggtgaca aaggcaccca attatccggc ggccaaaagc aaaggattgc
     3661 gattgcgcgc gccttggtgc ggcaacccca catcctctta ctggacgaag ccacctctgc
     3721 attagatact gaaagcgaga aagtggtgca agaagccctg gataaagcgc gcgagggcag
     3781 gacgtgtatc gtcattgctc ataggttgag cacaattcag aacgctgatc ttatcgtcgt
     3841 atttcaaaat ggacgtgtga aggaacatgg cactcatcaa caattgctgg cgcaaaaggg
     3901 catttacttt tctatggtat ctgtacaagc tggtacaaag agacaataac ttgtggaaag
     3961 gacgtgtcac cgctcaagtg gacccagctt tcttgtacaa agttggcatt ataagaaagc
     4021 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     4081 gaggctaggt ggaggctcag tgatgataag tctgcgatgg tggatgcatg tgtcatggtc
     4141 atagctgttt cctgtgtgaa attgttatcc gctcagaggg cacaatccta ttccgcgcta
     4201 tccgacaatc tccaagacat taggtggagt tcagttcggc gtatggcata tgtcgctgga
     4261 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     4321 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     4381 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     4441 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     4501 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     4561 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4621 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     4681 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     4741 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     4801 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     4861 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     4921 cctttgatct tttctacggg gtctgacgct ctattcaaca aagccgccgt cccgtcaagt
     4981 cagcgtaaat gggtaggggg cttcaaatcg tccgctctgc cagtgttaca accaattaac
     5041 aaattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg
     5101 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag
     5161 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc
     5221 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg
     5281 agtgacgact gaatccggtg agaatggcaa aagcttatgc atttctttcc agacttgttc
     5341 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat
     5401 tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac
     5461 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga
     5521 atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa
     5581 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt
     5641 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg
     5701 tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga
     5761 ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt
     5821 taatcgcggc ctcgagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt
     5881 actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat
     5941 gtaacatcag agattttgag acacaacgtg gctttccccc gccgctctag aactagtgga
     6001 tccaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     6061 cgcattatac gagacgtcca ggttgggata cctgaaacaa aacccatcgt acggccaagg
     6121 aagtctccaa taactgtgat ccaccacaag cgccagggtt ttcccagtca cgacgttgta
     6181 aaacgacggc cagtcatgca taatccgcac gcatctggaa taaggaagtg ccattccgcc
     6241 tgacct
//
