LOCUS       sequence-437191-        3115 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pENTR-SLC66A5_STOP
FEATURES             Location/Qualifiers
     source          1..3115
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(751..850)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(907..923)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(907..923)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(920..942)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1095..1683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1175..1194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1819..2634)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1819..2634)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2542..2561
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2626..2645
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     2755..2771
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2755..2771
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      2772..2818
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2932..2954
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2946..2963
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2947..2963
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3016..3115
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ccaccatggc agctgaggca gatggccctc tcaaaagact gcttgtaccg atcctgctgc
       61 ctgaaaaatg ctacgatcag ctgttcgtac agtgggatct cctccacgtg ccttgcctga
      121 aaatattgtt gtctaagggt ctgggactcg gcatcgtggc cgggtccctc ctggtaaagc
      181 tgcctcaagt ctttaagata ctgggtgcta aatccgccga gggactgtcc cttcaatccg
      241 tgatgctgga gctcgtggcg ttgaccggaa ccatggtgta ttcaatcact aataacttcc
      301 catttagctc ctggggggaa gccttattcc tgatgttgca gacgattacc atctgtttcc
      361 tggtaatgca ctaccggggc cagacagtca aaggagtcgc cttcctcgcc tgctatggcc
      421 tggtattgct ggtcttgctg tcccccctga cgcctctgac tgtagtcact ttgctccagg
      481 cgtcaaatgt cccagcagtt gtggtgggaa ggctgctgca agccgcaact aactatcaca
      541 acggacacac cggacagctg agcgcaataa cagtttttct cctgtttggt ggatccctgg
      601 cacgtatctt caccagcatc caggaaaccg gggaccccct gatggcaggt acctttgtag
      661 tatcctccct ttgtaatggg ctgatcgcag cacagttgct cttctactgg aatgctaagc
      721 caccacataa gcaaaagaaa gcccagtgag acccagcttt cttgtacaaa gttggcatta
      781 taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
      841 tcattatttg aggctaggtg gaggctcagt gatgataagt ctgcgatggt ggatgcatgt
      901 gtcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcagagggc acaatcctat
      961 tccgcgctat ccgacaatct ccaagacatt aggtggagtt cagttcggcg tatggcatat
     1021 gtcgctggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     1081 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     1141 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     1201 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     1261 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     1321 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1381 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     1441 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     1501 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     1561 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1621 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     1681 caagaagatc ctttgatctt ttctacgggg tctgacgctc tattcaacaa agccgccgtc
     1741 ccgtcaagtc agcgtaaatg ggtagggggc ttcaaatcgt ccgctctgcc agtgttacaa
     1801 ccaattaaca aattctgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
     1861 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
     1921 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
     1981 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
     2041 atcaccatga gtgacgactg aatccggtga gaatggcaaa agcttatgca tttctttcca
     2101 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
     2161 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca
     2221 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
     2281 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt
     2341 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
     2401 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
     2461 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt
     2521 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
     2581 gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt tgaatatggc tcataacacc
     2641 ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgatgata tatttttatc
     2701 ttgtgcaatg taacatcaga gattttgaga cacaacgtgg ctttcccccg ccgctctaga
     2761 actagtggat ccaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     2821 gttgtttgtc gcattatacg agacgtccag gttgggatac ctgaaacaaa acccatcgta
     2881 cggccaagga agtctccaat aactgtgatc caccacaagc gccagggttt tcccagtcac
     2941 gacgttgtaa aacgacggcc agtcatgcat aatccgcacg catctggaat aaggaagtgc
     3001 cattccgcct gacctcaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa
     3061 cagattgatg agcaatgctt ttttataatg ccaactttgt acaaaaaagc aggct
//
