LOCUS       sequence-437193-        3475 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pENTR-SLC66A4_STOP
FEATURES             Location/Qualifiers
     source          1..3475
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1111..1210)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1267..1283)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1267..1283)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1280..1302)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1455..2043)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1535..1554)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2179..2994)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2179..2994)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2902..2921
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2986..3005
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     3115..3131
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3115..3131
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      3132..3178
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3292..3314
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3306..3323
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3307..3323
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3376..3475
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ccaccatgat tcggaactgg cttacgatct ttatcctttt tcccttaaaa ttagtggaaa
       61 agtgtgagtc ctccgttagt ttaacagtgc caccagtcgt caaacttgaa aacgggagct
      121 ccaccaatgt ctccctcacc ttacgcccac ccctgaacgc tacgttagtc attacttttg
      181 agataacctt tcgaagtaaa aacattacca ttctcgaact gcctgacgag gtggtggtcc
      241 cccccggcgt gaccaatagc agcttccagg ttacctccca gaacgtggga caattgaccg
      301 tgtacctgca tggtaatcat agtaaccaga ccggccctag aatcaggttc ctggttatcc
      361 ggtcctccgc gatctctatt atcaaccaag tcatcgggtg gatttacttt gtcgcttgga
      421 gcattagctt ttacccccag gttatcatga attggagaag gaagtcagtc atcggactga
      481 gctttgactt cgtagctctg aacctcactg gcttcgtggc ctattcagtg ttcaacattg
      541 gactgctgtg ggtgccctat atcaaggagc agttcctgct caagtaccca aatggggtca
      601 accccgttaa ttctaatgat gtgttcttta gccttcacgc agtggtgttg acacttatca
      661 tcatagtaca gtgctgcttg tacgaacgcg ggggccagcg cgtctcatgg cctgcaatcg
      721 gcttcttagt actcgcctgg ctctttgcct tcgtaaccat gattgtggct gcagtggggg
      781 tcacgacctg gctgcagttt ctcttctgct tctcatatat taagctcgcc gtgaccctgg
      841 tgaaatactt cccacaggct tacatgaact tctactacaa atccactgag ggctggtcaa
      901 ttgggaatgt gctcttggat tttaccggcg gttccttttc attgcttcaa atgtttttac
      961 agagctataa caacgaccag tggaccctca tattcggaga tccaaccaag tttggtttgg
     1021 gcgtgtttag catagtcttt gacgtagtct tcttcattca gcacttctgc ctttacagga
     1081 aacggcctgg ctacgaccag cttaactagg acccagcttt cttgtacaaa gttggcatta
     1141 taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     1201 tcattatttg aggctaggtg gaggctcagt gatgataagt ctgcgatggt ggatgcatgt
     1261 gtcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcagagggc acaatcctat
     1321 tccgcgctat ccgacaatct ccaagacatt aggtggagtt cagttcggcg tatggcatat
     1381 gtcgctggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     1441 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     1501 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     1561 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     1621 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     1681 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1741 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     1801 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     1861 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     1921 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1981 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     2041 caagaagatc ctttgatctt ttctacgggg tctgacgctc tattcaacaa agccgccgtc
     2101 ccgtcaagtc agcgtaaatg ggtagggggc ttcaaatcgt ccgctctgcc agtgttacaa
     2161 ccaattaaca aattctgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
     2221 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
     2281 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
     2341 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
     2401 atcaccatga gtgacgactg aatccggtga gaatggcaaa agcttatgca tttctttcca
     2461 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
     2521 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca
     2581 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
     2641 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt
     2701 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
     2761 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
     2821 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt
     2881 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
     2941 gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt tgaatatggc tcataacacc
     3001 ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgatgata tatttttatc
     3061 ttgtgcaatg taacatcaga gattttgaga cacaacgtgg ctttcccccg ccgctctaga
     3121 actagtggat ccaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     3181 gttgtttgtc gcattatacg agacgtccag gttgggatac ctgaaacaaa acccatcgta
     3241 cggccaagga agtctccaat aactgtgatc caccacaagc gccagggttt tcccagtcac
     3301 gacgttgtaa aacgacggcc agtcatgcat aatccgcacg catctggaat aaggaagtgc
     3361 cattccgcct gacctcaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa
     3421 cagattgatg agcaatgctt ttttataatg ccaactttgt acaaaaaagc aggct
//
