LOCUS       sequence-437202-       12725 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TALEcharm expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12725)
  AUTHORS   Neumann EN, Bertozzi TM, Wu E, Serack F, Harvey JW, Brauer PP, 
            Pirtle CP, Coffey A, Howard M, Kamath N, Lenz K, Guzman K, Raymond 
            MH, Khalil AS, Deverman BE, Minikel EV, Vallabh SM, Weissman JS
  TITLE     Brainwide silencing of prion protein by AAV-mediated delivery of an 
            engineered compact epigenetic editor.
  JOURNAL   Science. 2024 Jun 28;384(6703):ado7082. doi: 
            10.1126/science.ado7082. Epub 2024 Jun 28.
  PUBMED    38935715
REFERENCE   2  (bases 1 to 12725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-06-28"; volume: "384(6703):ado7082. doi"; pages: " 
            10.1126/science.ado7082. Epub 2024 Jun 28"
COMMENT     Sequence Label: pENN456_TALEcharm_Kv2_MmT8x
FEATURES             Location/Qualifiers
     source          1..12725
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(114..133)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      927..1515
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1416..1435
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1669..1686
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1803..1824
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1839..1844,1845..1862,1863..1869)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1877..1893
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1882..1904
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1901..1917
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1901..1917
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1936..1956
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1938..1956
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1984..2210
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2211..2391
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2438..2563
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3056..3289
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3474..3518
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        3749..3959
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        3771..3967
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3818..3953
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3999..4601
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(3999..4018)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4495..4515
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4644..4761
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4899..6077
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5130..6068
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6025..6045
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      6085..6094
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6280..6465
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     CDS             9772..9792
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9793..9849
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    10561..11149
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10614..10634)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11032..11043)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             11221..11454
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11532..11653
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11569..11588)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11623..11642
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11693..11828
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11848..11867)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11849..11867)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11877..11894)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11877..11893)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11886..11908)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      12035..12490
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12122..12141)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12332..12353
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12516..12620
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12621..12689,12690..12725,1..756)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
       61 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      121 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      181 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      241 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      301 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      361 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      421 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
      481 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
      541 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
      601 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
      661 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
      721 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
      781 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
      841 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
      901 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
      961 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1021 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1081 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1141 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1201 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1261 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1321 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1381 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1441 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1501 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1561 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     1621 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     1681 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     1741 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     1801 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     1861 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     1921 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
     1981 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
     2041 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
     2101 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
     2161 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
     2221 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     2281 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     2341 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     2401 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     2461 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     2521 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     2581 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     2641 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     2701 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     2761 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     2821 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     2881 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     2941 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     3001 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     3061 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     3121 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     3181 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     3241 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     3301 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     3361 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     3421 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     3481 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     3541 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     3601 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     3661 ttcagaccca cctcccaacc ccgaggggac ccatgcattg catctcaatt agtcagcaac
     3721 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcgtctcaa
     3781 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     3841 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     3901 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     3961 ttgcaaaaag ctttctagag gtaccaccgg agcttaccat gaccgagtac aagcccacgg
     4021 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     4081 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     4141 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     4201 acgacggcgc cgccgtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     4261 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     4321 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     4381 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     4441 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     4501 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     4561 cgcgcacctg gtgcatgacc cgcaagcccg gtgccagtta acgaattcta gatcttgaga
     4621 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     4681 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     4741 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc
     4801 cggctcgagg cctgcaggtg caaagatgga taaagtttta aacagagagg aatctttgca
     4861 gctaatggac cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg
     4921 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     4981 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     5041 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     5101 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     5161 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     5221 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     5281 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     5341 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     5401 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     5461 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     5521 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     5581 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg
     5641 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     5701 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     5761 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     5821 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     5881 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     5941 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     6001 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     6061 catttcaggt gtcgtgaggg atccgccacc atggccagga ccaaacaaac tgccagaaag
     6121 agcaccgggg ggaaagcgcc ccgaaaacag ctggcgacca aagcagcccg caaaagcgca
     6181 ccgggaggtg caagcagtgg ggcaggaagt tcctcaggag gaagtgcggc gggatccgga
     6241 agcggtggcg catcaagtgg cgccgcaagt agcagtgcca gaaccctggt gaccttcaag
     6301 gacgtgttcg tggacttcac cagagaagaa tggaagctgc tggataccgc tcagcagatc
     6361 gtgtacagaa acgtgatgct ggaaaactac aagaatctgg tgtccctggg ctaccagctg
     6421 accaagcctg acgtgatcct gagactggaa aagggagagg agccttccgg ctctgaaacc
     6481 cctggaacct ccgagagcgc cacacctgaa agctcccctg ttgagatcta caagaccgtg
     6541 tccgcctgga agcggcagcc aatcagagtg ctgagcctgt tcggcaatat tgacaaggaa
     6601 ctgaagtctc tgggcttcct ggaaagcggc agccacagcg agggcggcac actgaagtac
     6661 gtggaagatg tgaccaacgt ggtgcggaga gatgtggaaa agtggggacc tttcgacctg
     6721 ctgtacggca gcacccagcc tctgggcgga agctgcgaca ggtgtcctgg ctggtacatg
     6781 ttccaatttc atagaatcct gcagtacgcc agacctagac aggagtctca gcccttcttc
     6841 tggatcttca tggataacct gctgctcaca gaggacgacc agatgaccac cgtgcggttc
     6901 ctgcaaacag aggccgtcac cctgcgcgac gtgagatcta gagtgctgca gaacgccgtc
     6961 cgggtgtgga gcaacatccc cggtcttaaa agcaagcacg aggctctgtc cccaaaagag
     7021 gaagagagcc tgcagggcca cgtgcggacc agatctaagc tggccgctcc taaagtggac
     7081 cccctggtga agaactgcct gctgcctctg agagagtact ttaagtattt cagccagagc
     7141 agcctgcccc tgggcggccc ttctagcggt gctccacctc caagcggcgg ctcgccagcc
     7201 ggcagcccta ccagcaccga ggagggcaca agcgagagcg caacacctga gagcggcccc
     7261 ggcacaagca ccgagccttc tgaaggaagc gcccctggga gccctgccgg cagccccacc
     7321 agcacagaag agggcacctc taccgagccc agcgagggta gcgcccctgg cacaagcaca
     7381 gaaccctctg agaagcggac tgccgatgga tctgagtttg agagccctaa gaaaaaaaaa
     7441 aaggttggca ttcaccgcgg ggtacctatg gtggacttga ggacactcgg ttattcgcaa
     7501 cagcaacagg agaaaatcaa gcctaaggtc aggagcaccg tcgcgcaaca ccacgaggcg
     7561 cttgtggggc atggcttcac tcatgcgcat attgtcgcgc tttcacagca ccctgcggcg
     7621 cttgggacgg tggctgtcaa ataccaagat atgattgcgg ccctgcccga agccacgcac
     7681 gaggcaattg taggggtcgg taaacagtgg tcgggagcgc gagcacttga ggcgctgctg
     7741 actgtggcgg gtgagcttag ggggcctccg ctccagctcg acaccgggca gctgctgaag
     7801 atcgcgaaga gagggggagt aacagcggta gaggcagtgc acgcctggcg caatgcgctc
     7861 accggggccc ccttgaacct tacgccagac caggtcgttg ccatcgcaag ccacgacgga
     7921 ggaaaacagg ctctggagac agtccagaga cttcttccgg tgttgtgcca ggaccacggt
     7981 ttttctcagg ccgacatcgt gaaaatcgcc ggtcatgatg gtggcgcaca ggccctacag
     8041 gcggtactgg aattggaacc caccctgcgc gaaaggggac tcacaccaca acaggtggtc
     8101 gccatagcta atggcggagg aaagcaggct cttgagactg tgcagcgcct gctcccagtt
     8161 ctgtgtcagg ctcatggcct caccccagat caggtggttg ccatcgcttc taaccacggg
     8221 ggcaaacaag ccctggagac tgtccaacgg ctgctcccgg ttttgtgcca ggatcacggc
     8281 cttaccccag cacaggtcgt tgccattgca tccaatcatg gcggtaaaca ggctctggaa
     8341 actgtccaag cgttgctgcc tgtactctgt caggaccacg gattgacccc tgaccaagtc
     8401 gttgcaatcg caaatcacga cggcgggaag caggctctgg aaactgttca aagactcctt
     8461 cccgttctct gtcaggcaca cggactgacc ccagaacagg tcgtggcaat cgcttcaaat
     8521 ggcggaggca aacaggctct cgaaacagtg cagaggctcc ttccggtcct gtgtcaggcc
     8581 cacggattct cacaggcaga catcgtgaag attgctggcc acgatggtgg cgcgcaagca
     8641 ctccaagcgg tgttggaatt ggagcccact ctccgggaaa gggggctcac gcccgatcaa
     8701 gtagtggcaa ttgcaagtca cgacggagga aaacaagctc ttgaaaccgt gcaaagactc
     8761 cttccggtgt tgtgtcaaga tcatggcctg acgccggccc aagttgtggc cattgcttct
     8821 catgacgggg gtaagcaagc ccttgaaact gtccaggcct tgctgcccgt gctgtgtcaa
     8881 gctcacggac tcacaccaga ccaagtggtt gccattgcaa atcacgatgg aggaaaacaa
     8941 gcgttggaga ctgttcaacg attgcttcca gtgctttgtc aagcccatgg tctgacacct
     9001 gatcaagttg ttgctatcgc aaatggcgga ggtaaacaag ctttagaaac agtacaacgg
     9061 ttgctacctg ttctttgcca agctcacggt ctgacgcccg agcaggtagt agccatagct
     9121 tctaatcacg gcggaaagca agcactggag accgtccagc gcctccttcc tgtactctgc
     9181 caagcgcacg ggttgacgcc tcaacaagta gtggcaatcg cgaacggggg aggcaaacag
     9241 gctctggaga cagttcaaag gcttctccca gtgttgtgtc aagcacacgg ttactccaga
     9301 gcggatattg tcaaaattgc cggaaatcac ggtggggcgc aggcccttaa agccgtagtg
     9361 atgcacggac ctacgctttg cgaacgcgga ctcacgccgg atcaggttgt tgccatagct
     9421 aataacggag gggggaaaca agctttggaa acagttcaaa gacttcttcc tgtgctctgt
     9481 caggaccacg ggcttacgcc acaacaggtg gtggcgattg catctaatgg aggcggacgc
     9541 cctgccttgg agtccatcgt agcccaattg tccaggcccg atcccgcgtt ggctgcgtta
     9601 acgaatgacc atctggtggc gttggcatgt cttggtggac gacccgcgct cgatgcagtc
     9661 aaaaagggtc tgcctcatgc tcccgcattg atcaaaagaa ccaaccggcg gattcccgag
     9721 agaacttccc atcgagtcgc gaagaggacc gccgacggca gcgagttcga gcccaagaaa
     9781 aagcggaagg tggctacaaa cttcagcctg ctgaaacagg ccggcgacgt cgaggaaaat
     9841 cctggcccca gcgagctgat taaggaaaac atgcacatga agctgtatat ggaaggcacc
     9901 gtggacaacc accatttcaa gtgcaccagc gagggcgaag gcaagcccta cgagggaaca
     9961 cagaccatgc ggatcaaggt ggtggaaggc ggccctctgc cattcgcctt cgacatcctc
    10021 gccacctctt tcctgtacgg cagtaagacc tttatcaacc acacccaggg catccccgat
    10081 ttcttcaagc agagcttccc cgagggattt acctgggaga gagtgaccac ctacgaggac
    10141 ggcggcgtgc tgacagccac ccaggacacc agcctgcagg acggctgtct gatctacaac
    10201 gtgaagatca gaggcgtgaa cttcaccagc aacggccctg tgatgcagaa aaaaaccctg
    10261 ggctgggagg cctttacaga gacactgtac cccgctgatg gaggcctgga aggtagaaac
    10321 gacatggccc tgaaactggt cggcggcagc cacctgattg ccaatatcaa gaccacatac
    10381 cggagcaaga aaccagctaa aaacctgaag atgcctggcg tttactacgt ggattacaga
    10441 ctggagcgga tcaaggaagc caacaacgag acatatgtgg aacagcacga ggtggccgta
    10501 gctagatact gcgacctgcc ttctaagctt ggccacaagc tgaactaagc tagcgtcgac
    10561 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
    10621 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
    10681 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
    10741 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
    10801 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
    10861 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
    10921 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
    10981 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
    11041 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
    11101 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
    11161 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
    11221 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
    11281 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
    11341 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
    11401 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
    11461 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
    11521 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
    11581 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    11641 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
    11701 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
    11761 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
    11821 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
    11881 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
    11941 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
    12001 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
    12061 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
    12121 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
    12181 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
    12241 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
    12301 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
    12361 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
    12421 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
    12481 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
    12541 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
    12601 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
    12661 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
    12721 ctgaa
//
